REL
2024-03-14
2025-10-15
2025-10-15
Cryo-electron tomography of mitotic chromosomes isolated from chicken DT-40 cells with annotated nucleosome positions
0000-0003-2298-8964
Mikhail
Eltsov
Department of Integrated Structural Biology, Institute of Genetics and Molecular and Cellular Biology (IGBMC)
1 rue Laurent Fries
Illkirch
France
67404
0000-0003-2298-8964
Mikhail
Eltsov
Department of Integrated Structural Biology, Institute of Genetics and Molecular and Cellular Biology (IGBMC)
1 rue Laurent Fries
Illkirch
France
67404
0000-0001-6263-0452
Charles
Kervrann
Centre INRIA de l’Université de Rennes
Campus Universitaire de Beaulieu
35042 Rennes
France
35042
Patra G
Harastani M
Eltsov M
Agence Nationale de la Recherche (ANR)
ANR-20-CE11-0020-02
France
Agence Nationale de la Recherche (ANR)
ANR-23-CE45-0012-01
France
6.4
10.6019/EMPIAR-11969
EMDB
molecule
EMD-19823
EMD-19825
Harastani M
Patra G
Kervrann C
Eltsov M
Template Learning: Deep learning with domain randomization for particle picking in cryo-electron tomography
Nature communications
Nat Commun
France
1
16
2025
English
10.1038/s41467-025-63895-0
41044063
Unaligned multiframe tilt images of mitotic chromosomes
/data/Frames
micrographs - multiframe
TIFF
TIFF
41
10
1
10
UNSIGNED BYTE
5760
2.075
4092
2.075
Raw unaligned multi-frame micrographs were aligned, sorted and combined into tilt series stacks using Motioncor2. The tilt series were reconstructed using etomo (IMOD).
Original metadata files (.mdoc) are provided in the mdoc folder and tomograms reconstructed using etomo (IMOD) (.mrc file in Tomogram folder). The alignment information (.xf file) is in the Alignment_info folder.
data/Frames/ts_160_00**.tif
Aligned tilt series of mitotic chromosome
/data/TiltSeries
tilt series
MRCS
MRCS
1
41
1
41
32 BIT FLOAT
4092
2.075
5760
2.075
Tilt series stack (.mrcs file) aligned with etomo (IMOD).
Reconstructed tomogram of mitotic chromosome
/data/Tomogram
reconstructed volumes
MRC
MRC
1
1
16 BIT FLOAT
1023
8.3
1440
8.3
Tomogram (4-times binned reconstructions, original pixel size 2.075 Å) from aligned tilt series (.mrcs file in TiltSeries folder). The coordinates of nucleosomes annotated by Template Learning and template matching are in the picks folder.