REL
2024-01-16
2024-05-20
2024-05-20
Structure of an ALYREF-exon junction complex hexamer
0000-0001-5975-5609
Matthias
Kopano
Vorländer
Forschungsinstitut für Molekulare Pathologie
Campus-Vienna-Biocenter 1
Wien
Austria
1030
0000-0002-6020-9514
Clemens
Plaschka
Forschungsinstitut für Molekulare Pathologie
Campus-Vienna-Biocenter 1
Wien
Austria
1030
Pacheco-Fiallos B
Vorländer MK
Plaschka C
European Research Council (ERC)
ERC-2020-STG 949081 RNApaxport
Belgium
Welch Foundation
203149
United Kingdom
European Molecular Biology Organization (EMBO)
ALTF 623-2020
Germany
European Research Council (ERC)
MCSA 101028744
Belgium
6.6
10.6019/EMPIAR-12036
EMDB
molecule
EMD-14803
Pacheco-Fiallos B
Vorländer MK
Riabov-Bassat D
Fin L
O'Reilly FJ
Ayala FI
Schellhaas U
Rappsilber J
Plaschka C
mRNA recognition and packaging by the human transcription-export complex
Nature
Nature
7958
616
828
835
2023
English
10.1038/s41586-023-05904-0
37020021
Unaligned multi-frame micrographs of the ALYREF-EJX hexamer in EER format
/data/ALYREF-EJC/frames
micrographs - multiframe
EER
EER
7941
1673
UNSIGNED BYTE
4096
0.945
4096
0.945
Raw EER files from Falcon 4 detector, acquired with EPU and a total dose of 40 e-/Å2
data/ALYREF-EJC/frames/*.eer
Motion-corrected and dose-weighted micrographs
/data/ALYREF-EJC/doseweighted_completed
micrographs - single frame
MRC
MRC
7941
1
32 BIT FLOAT
4096
0.945
4096
0.945
Motion-corrected and dose-weighted micrographs
data/ALYREF-EJC/doseweighted_completed/*doseweighted.mrc
Motion-corrected micrographs
/data/ALYREF-EJC/patch_aligned_completed
micrographs - single frame
MRC
MRC
7941
1
32 BIT FLOAT
4096
0.945
4096
0.945
Motion-corrected micrographs without dose-weighting, used for CTF estimation
data/ALYREF-EJC/patch_aligned_completed/*aligned.mrc
Final particles used for reconstruction
/data/ALYREF-EJC/particle_stack/restack
picked particles - multiframe - processed
MRC
MRC
1564602
10000
16 BIT FLOAT
320
0.945
320
0.945
Final particles used for reconstrution
data/ALYREF-EJC/particle_stack/restack/*mrc
data/ALYREF-EJC/particle_stack/J66_passthrough_particles.cs
CryoSPARC job of the final 3D reconstruction
/data/ALYREF-EJC/reconstruction_job
reconstructed volumes
MRC
MRC
1564602
1
16 BIT FLOAT
variable
variable
variable
variable
CryoSPARC job of the final 3D reconstruction
Gainref file for motion correction of compressed movies
/data/ALYREF-EJC/gainref
micrographs - single frame
MRC
MRC
1
1
32 BIT FLOAT
4096
0.945
4096
0.945
Gainref file for motion correction
data/ALYREF-EJC/gainref/gainref/20220315_162118_EER_GainReference.gain