CryoEM movies of human tau amyloid fibrils extracted from an AD patient brain (in association with in situ cryoET)

REL 2024-04-15 2024-09-03 2024-09-03 CryoEM movies of human tau amyloid fibrils extracted from an AD patient brain (in association with in situ cryoET) 0000-0001-9724-9547 René AW Frank University of Leeds Woodhouse Leeds Yorkshire United Kingdom LS2 9JT 0000-0001-9724-9547 René AW Frank University of Leeds Woodhouse Leeds United Kingdom LS2 9JT Gilbert MAG Fatima N Jenkins J O'Sullivan TJ Schertel A Halfon Y Wilkinson M Morrema THJ Geibel M Read RJ Ranson NA Radford SE Hoozemans JJM Frank RAW Wellcome Trust 204963 United Kingdom UK Research and Innovation (UKRI) MR/V022644/1 United Kingdom 1.4 10.6019/EMPIAR-12045 EMDB molecule EMD-18990 Gilbert MAG Fatima N Jenkins J O'Sullivan TJ Schertel A Halfon Y Wilkinson M Morrema THJ Geibel M Read RJ Ranson NA Radford SE Hoozemans JJM Frank RAW CryoET of β-amyloid and tau within postmortem Alzheimer's disease brain Nature Nature 8022 631 913 919 2024 English 10.1038/s41586-024-07680-x 38987603

10,861 unaligned, gain corrected tif movies are supplied, each containing 38 frames with a dose per frame of ~1.15 e-/Å2. The pixel size is 0.83 Å, data collected on Krios-Falcon4.

Unaligned multi-frame Tif movies of extracted amyloid fibrils from post-mortem human AD patient brain /data/Movies_tifs micrographs - multiframe TIFF TIFF 10860 38 UNSIGNED BYTE 4096 0.83 4096 0.83

Movie stacks are gain corrected but unaligned, 10,860 movies, each containing 38 tiff frames with a dose per frame of ~1.15 e-/Å2. The total cumulative dose for each movie is ~44 e-/Å2 over a 4 s exposure. Predominantly Tau fibrils present, also meshes of abeta fibrils and a small percentage of fibrils that resemble TMEM106B from 2D class averages.