REL 2023-05-10 2026-03-30 2026-03-30 Movies of PARP1-DNA Complexes 0000-0003-1405-925X Aleksandr Sverzhinsky Department of Biochemistry and Molecular Medicine, Universite de Montreal 2900 Edouard Montpetit Blvd Montreal Quebec Canada H3T 1J4 0000-0002-2714-4317 John M Pascal Department of Biochemistry and Molecular Medicine, Universite de Montreal 2900 Edouard Montpetit Blvd Montreal Quebec Canada H3T 1J4 Sverzhinsky A Pascal JM 27.2 10.6019/EMPIAR-12081 EMDB molecule EMD-48285 EMD-48313 Sverzhinsky A Xue H Langelier MF Muniz Corrêa MV Del Mundo J Classen S Hammel M Rothenberg E Pascal JM PARP1-HPF1 structure and dynamics on nicked DNA suggest a mechanism for acute and localized ADP-ribosylation Nature communications Nat Commun 1 17 2026 English 10.1038/s41467-026-69375-3 41698892 Unaligned multi-frame micrographs of PARP1-DNA-EB47 on graphene by vitrobot /data/frames_PARP1-DNA-HPF1-Tmls-EB47_550ms micrographs - multiframe TIFF TIFF 24130 40 UNSIGNED BYTE 5760 1.069 4092 1.069
The movies were imaged (Titan Krios) at a nominal pixel resolution of 1.069 A/pix using a Gatan K3 direct electron detector in counting mode. Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 26.38 e-/Å^2/s with a total electron dose of 52.77 e-/Å^2.
 Unaligned multi-frame micrographs of PARP1-DNA-EB47-HPF1-Timeless on graphene by vitrobot /data/frames_PARP1-DNA-HPF1-Tmls-EB47_550ms micrographs - multiframe EER EER 9643 60 UNSIGNED BYTE 4096 0.751 4096 0.751
The movies were imaged (Titan Krios) at a nominal pixel resolution of 0.7511 A/pix using a Falcon 4EC direct electron detector (Thermo Fisher Scientific) in counting mode. Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 7.18 e-/Å^2/s with a total electron dose of 43.08 e-/Å^2.
 Unaligned multi-frame micrographs of PARP1-DNA-EB47-HPF1-Timeless (crosslinked) on graphene by vitrobot /data/frames_PARP1-DNA-HPF1-Tmls-EB47_550ms micrographs - multiframe TIFF TIFF 8600 40 UNSIGNED BYTE 5760 1.0691 4092 1.0691
The movies were imaged (Titan Krios) at a nominal pixel resolution of 1.069 A/pix using a Gatan K3 direct electron detector in counting mode. Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 26.38 e-/Å^2/s with a total electron dose of 52.77 e-/Å^2.
 Unaligned multi-frame micrographs of PARP1-DNA-EB47-HPF1-Timeless (crosslinked) on graphene by vitrobot; tilted collection /data/frames_PARP1-DNA-HPF1-Tmls-EB47_550ms micrographs - multiframe TIFF TIFF 15299 40 UNSIGNED BYTE 5760 1.069 4092 1.069
The movies were imaged (Titan Krios) at a nominal pixel resolution of 1.069 A/pix using a Gatan K3 direct electron detector (Thermo Fisher Scientific) in counting mode at a tilt of 30 degrees. Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 26.2 e-/Å^2/s with a total electron dose of 83.82 e-/Å^2.
 Unaligned multi-frame micrographs of PARP1-DNA-EB47-HPF1-Timeless on graphene by Chameleon; grid1 /data/frames_PARP1-DNA-HPF1-Tmls-EB47_550ms micrographs - multiframe EER EER 9643 60 UNSIGNED BYTE 4096 0.7511 4096 0.7511
The movies were imaged (Titan Krios) at a nominal pixel resolution of 0.7511 A/pix using a Falcon 4EC direct electron detector (Thermo Fisher Scientific) in counting mode. Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 7.47 e-/Å^2/s with a total electron dose of 44.81 e-/Å^2.
 Unaligned multi-frame micrographs of PARP1-DNA-EB47-HPF1-Timeless on graphene by Chameleon; grid2 /data/frames_PARP1-DNA-HPF1-Tmls-EB47_550ms micrographs - multiframe EER EER 7445 70 UNSIGNED BYTE 4096 0.7511 4096 0.7511
The movies were imaged (Titan Krios) at a nominal pixel resolution of 0.7511 A/pix using a Falcon 4EC direct electron detector (Thermo Fisher Scientific) in counting mode. Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 7.90 e-/Å^2/s with a total electron dose of 55.27 e-/Å^2.
 Unaligned multi-frame micrographs of PARP1-DNA-EB47-HPF1-Timeless in open-hole by Chameleon /data/frames_PARP1-DNA-HPF1-Tmls-EB47_550ms micrographs - multiframe TIFF TIFF 4246 40 UNSIGNED BYTE 11520 0.548 8184 0.548
The movies were imaged (Talos Arctica) at a nominal pixel resolution of 0.548 A/pix using a K2 Summit direct electron detector (Gatan) in counting mode. Movies were collected using Leginon (Suloway et al., 2005) with a total electron dose of 50.44 e-/Å^2.