REL 2024-03-24 2024-11-06 2024-11-06 0000-0002-8547-7523 Taco W.A. Kooij Department of Medical Microbiology, Radboudumc, Nijmegen Nijmegen Netherlands 6500 HB 0000-0002-8547-7523 Taco W.A. Kooij Department of Medical Microbiology, Radboudumc, Nijmegen Nijmegen Netherlands 6500 HB Evers F Roverts R Boshoven C Lindert MK Sinden RE Akiva A Kooij TWA Verhoef JMJ Sommerdijk N Netherlands Organisation for Scientific Research (NWO) NWO-VIDI 864.13.009 Netherlands 239.1 10.6019/EMPIAR-12160 FIB-SEM cell Evers F Roverts R Boshoven C Kea-te Lindert M Verhoef JM Sinden RE Akiva A Kooij TW bioRxiv 2023 English 10.1101/2023.03.10.531920

Deposited are the raw micrographs underlying the findings presented in 10.1101/2023.03.10.531920. Samples are derived from P. falciparum gametocytes. Crops of individual cells that were rendered or used for analysis in the manuscript are also provided.

Pf_Gametocyte_#3 /data/EMPIAR_Submission/IndividualCells/GAM3 micrographs - multiframe TIFF TIFF 499 1 1 499 UNSIGNED BYTE 1833 50.0 893 50.0

Micrograph series of Plasmodium falciparum gametocyte. Representative cell depicted in Fig. 1A. Voxel Size 5nm x 5nm x 15nm

Raw Image Stack Gametocyte Sample 1 /data/EMPIAR_Submission/Stacks/Sample1 micrographs - multiframe TIFF TIFF 2627 1 1 2627 UNSIGNED BYTE 4268 50.0 2784 50.0

Raw micrograph series for gametocyte sample #1. Voxel Size 5nm x 5nm x 15nm

Raw Image Stack Gametocyte Sample 2 /data/EMPIAR_Submission/Stacks/Sample2 micrographs - multiframe TIFF TIFF 952 1 1 952 UNSIGNED BYTE 4428 50.0 3804 50.0

Raw micrograph series for gametocyte sample #2. Voxel Size 5nm x 5nm x 15nm

Raw Image Stack Gametocyte Sample 3 /data/EMPIAR_Submission/Stacks/Sample3 micrographs - multiframe TIFF TIFF 901 1 1 901 UNSIGNED BYTE 4405 50.0 2880 50.0

Raw micrograph series for gametocyte sample #3. Voxel Size 5nm x 5nm x 15nm

Pf_Gametocyte_#16 /data/EMPIAR_Submission/IndividualCells/GAM16 micrographs - multiframe TIFF TIFF 804 1 1 804 UNSIGNED BYTE 1296 50.0 1392 50.0

Micrograph series of Plasmodium falciparum gametocyte. Representative cell depicted in Fig. 1B. Voxel Size 5nm x 5nm x 15nm

Pf_Schizont_#1 /data/EMPIAR_Submission/IndividualCells/ABS1 micrographs - multiframe TIFF TIFF 410 1 1 410 UNSIGNED BYTE 1230 50.0 1386 50.0

Micrograph series of Plasmodium falciparum schizont. Representative cell depicted in Fig. 1C. Voxel Size 5nm x 5nm x 15nm

Pf_Schizont_#12 /data/EMPIAR_Submission/IndividualCells/ABS12 micrographs - multiframe TIFF TIFF 421 1 1 421 UNSIGNED BYTE 1476 50.0 1314 50.0

Micrograph series of Plasmodium falciparum schizont. Representative cell depicted in Fig. 1D. Voxel Size 5nm x 5nm x 15nm

Pf_Gametocyte_#9 /data/EMPIAR_Submission/IndividualCells/GAM9 micrographs - multiframe TIFF TIFF 594 1 1 594 UNSIGNED BYTE 1416 50.0 1686 50.0

Micrograph series of Plasmodium falciparum Gametocyte. Representative cell depicted in Fig. 4C / Supp. Fig. 2 Row 2. Voxel Size 5nm x 5nm x 15nm

Pf_Gametocyte_#4 /data/EMPIAR_Submission/IndividualCells/GAM4 micrographs - multiframe TIFF TIFF 624 1 1 624 UNSIGNED BYTE 1984 50.0 1060 50.0

Micrograph series of Plasmodium falciparum Gametocyte. Representative cell depicted in Supp Fig.1A Upper Cell. Voxel Size 5nm x 5nm x 15nm

Pf_Gametocyte_#13 /data/EMPIAR_Submission/IndividualCells/GAM13 micrographs - multiframe TIFF TIFF 405 1 1 405 UNSIGNED BYTE 1290 50.0 2010 50.0

Micrograph series of Plasmodium falciparum Gametocyte. Representative cell depicted in Supp Fig.1A Lower Cell. Voxel Size 5nm x 5nm x 15nm

Pf_Gametocyte_#1 /data/EMPIAR_Submission/IndividualCells/GAM1 micrographs - multiframe TIFF TIFF 568 1 1 568 UNSIGNED BYTE 2596 50.0 1028 50.0

Micrograph series of Plasmodium falciparum Gametocyte. Representative cell depicted in Supp Fig.2 First Row. Voxel Size 5nm x 5nm x 15nm

Pf_Gametocyte_#2 /data/EMPIAR_Submission/IndividualCells/GAM2 micrographs - multiframe TIFF TIFF 520 1 1 520 UNSIGNED BYTE 1536 50.0 1128 50.0

Micrograph series of Plasmodium falciparum Gametocyte. Representative cell depicted in Supp Fig.2 Third Row. Voxel Size 5nm x 5nm x 15nm

Pf_Gametocyte_#7 /data/EMPIAR_Submission/IndividualCells/GAM7 micrographs - multiframe TIFF TIFF 643 1 1 643 UNSIGNED BYTE 1368 50.0 990 50.0

Micrograph series of Plasmodium falciparum Gametocyte. Representative cell depicted in Supp Fig.2 Fourth Row. Voxel Size 5nm x 5nm x 15nm

Pf_Gametocyte_#10 /data/EMPIAR_Submission/IndividualCells/GAM10 micrographs - multiframe TIFF TIFF 677 1 1 677 UNSIGNED BYTE 2568 50.0 1146 50.0

Micrograph series of Plasmodium falciparum Gametocyte. Representative cell depicted in Supp Fig.2 Fifth Row. Voxel Size 5nm x 5nm x 15nm

Pf_Gametocyte_#15 /data/EMPIAR_Submission/IndividualCells/GAM15 micrographs - multiframe TIFF TIFF 590 1 1 590 UNSIGNED BYTE 1314 50.0 1596 50.0

Micrograph series of Plasmodium falciparum Gametocyte. Representative cell depicted in Supp Fig.6. Voxel Size 5nm x 5nm x 15nm

Pf_Gametocyte_#18 /data/EMPIAR_Submission/IndividualCells/GAM18 micrographs - multiframe TIFF TIFF 566 1 1 566 UNSIGNED BYTE 2616 50.0 1128 50.0

Micrograph series of Plasmodium falciparum Gametocyte. Representative cell depicted in Supp Fig.11B. Voxel Size 5nm x 5nm x 15nm

Pf_Gametocyte_#5 /data/EMPIAR_Submission/IndividualCells/GAM5 micrographs - multiframe TIFF TIFF 614 1 1 614 UNSIGNED BYTE 1996 50.0 752 50.0

Micrograph series of Plasmodium falciparum Gametocyte. Representative cell depicted in Supp Fig.11C. Voxel Size 5nm x 5nm x 15nm

Pf_Gametocyte_#6 /data/EMPIAR_Submission/IndividualCells/GAM6 micrographs - multiframe TIFF TIFF 414 1 1 414 UNSIGNED BYTE 2028 50.0 1028 50.0

Micrograph series of Plasmodium falciparum Gametocyte. Cell was used for nuclear rendering in Fig. 8. Voxel Size 5nm x 5nm x 15nm

Pf_Gametocyte_#8 /data/EMPIAR_Submission/IndividualCells/GAM8 micrographs - multiframe TIFF TIFF 973 1 1 973 UNSIGNED BYTE 2178 50.0 1356 50.0

Micrograph series of Plasmodium falciparum Gametocyte. Cell was used for nuclear rendering in Fig. 8. Voxel Size 5nm x 5nm x 15nm

Pf_Gametocyte_#11 /data/EMPIAR_Submission/IndividualCells/GAM11 micrographs - multiframe TIFF TIFF 505 1 1 505 UNSIGNED BYTE 2016 50.0 846 50.0

Micrograph series of Plasmodium falciparum Gametocyte. Cell was used for nuclear rendering in Fig. 8. Voxel Size 5nm x 5nm x 15nm

Pf_Gametocyte_#19 /data/EMPIAR_Submission/IndividualCells/GAM19 micrographs - multiframe TIFF TIFF 511 1 1 511 UNSIGNED BYTE 2064 50.0 918 50.0

Micrograph series of Plasmodium falciparum Gametocyte. Cell was used for nuclear rendering in Fig. 8. Voxel Size 5nm x 5nm x 15nm

Pf_Gametocyte_#14 /data/EMPIAR_Submission/IndividualCells/GAM14 micrographs - multiframe TIFF TIFF 267 1 1 267 UNSIGNED BYTE 1620 50.0 1794 50.0

Micrograph series of Plasmodium falciparum Gametocyte. Cell was used for nuclear rendering in Fig. 8. Voxel Size 5nm x 5nm x 15nm

Sample6_UnprocessedStack_Inlens_P1 /data/EMPIAR_Submission/Stacks/Sample6 micrographs - multiframe TIFF TIFF 348 1 1 348 UNSIGNED BYTE 13044 50.0 4019 50.0

Raw micrograph series for Sample #6. Part 1/3. Voxel Size 5nm x 5nm x 15nm

Sample6_UnprocessedStack_InLens_P2 /data/EMPIAR_Submission/Stacks/Sample6 micrographs - multiframe TIFF TIFF 1423 1 1 1423 UNSIGNED BYTE 12893 50.0 3728 50.0

Raw micrograph series for Sample #6. Part 2/3. Voxel Size 5nm x 5nm x 15nm

Sample6_UnprocessedStack_InLens_P3 /data/EMPIAR_Submission/Stacks/Sample6 micrographs - multiframe TIFF TIFF 1234 1 1 1234 UNSIGNED BYTE 10487 50.0 4683 50.0

Raw micrograph series for Sample #6. Part 3/3. Voxel Size 5nm x 5nm x 15nm

Gametocytes #20-#50 /data/EMPIAR_Submission/IndividualCells/AdditionalGAM micrographs - multiframe TIFF TIFF 31 1 UNSIGNED BYTE variable 50.0 variable 50.0

Image Stacks corresponding to additional gametocyte stacks that were used for quantification but no rendered in their entirety in any figures. Voxel Size 5nm x 5nm x 15nm

Aligned Serial Sectioning Image Stack Gametocytes /data/EMPIAR_Submission/Stacks/SerialSectioning micrographs - multiframe TIFF TIFF 47 1 1 47 UNSIGNED BYTE 9077 50.0 14470 50.0

Aligned serial sectioning micrograph series for Sample #1. Voxel Size 5nm x 5nm x 80nm