<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-12165" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2024-06-26</depositionDate>
            <releaseDate>2025-08-13</releaseDate>
            <updateDate>2025-08-13</updateDate>
        </keyDates>
        <title>Cryo-EM structures of AceCas9</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0003-2046-9861</authorORCID>
            <firstName>Hong</firstName>
            <lastName>Li</lastName>
            <organization type="academic">Florida State University</organization>
            <street>91 Chieftan Way</street>
            <townOrCity>Tallahassee</townOrCity>
            <stateOrProvince>FL</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>32306</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0003-2046-9861</authorORCID>
            <firstName>Hong</firstName>
            <lastName>Li</lastName>
            <organization type="academic">Florida State University</organization>
            <street>91 Chieftan Way</street>
            <townOrCity>Tallahassee</townOrCity>
            <stateOrProvince>FL</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>32306</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author>Das A</author>
            <author>Rai J</author>
            <author>Roth MO</author>
            <author authorORCID="0009-0000-8986-0725">Shu Y</author>
            <author>Medina ML</author>
            <author>Barakat MR</author>
            <author authorORCID="0000-0003-2046-9861">Li H</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>National Institutes of Health (NIH)</fundingBody>
                <code>R01GM101343</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health (NIH)</fundingBody>
                <code>S10RR025080</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health (NIH)</fundingBody>
                <code>U24GM116788</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health (NIH)</fundingBody>
                <code>S10RR024564</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health (NIH)</fundingBody>
                <code>S10 RR024564</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>Department of Energy (DOE, United States)</fundingBody>
                <code>KP160711</code>
                <country>United States</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">6.3</datasetSize>
        <entryDOI>10.6019/EMPIAR-12165</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-27143</emdbEntry>
            <emdbEntry>EMD-27142</emdbEntry>
            <emdbEntry>EMD-27141</emdbEntry>
            <emdbEntry>EMD-27146</emdbEntry>
            <emdbEntry>EMD-27144</emdbEntry>
            <emdbEntry>EMD-27145</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author order="1">Das A</author>
                    <author order="2">Rai J</author>
                    <author order="3">Roth MO</author>
                    <author authorORCID="0009-0000-8986-0725" order="4">Shu Y</author>
                    <author order="5">Medina ML</author>
                    <author order="6">Barakat MR</author>
                    <author authorORCID="0000-0003-2046-9861" order="7">Li H</author>
                    <title>Coupled catalytic states and the role of metal coordination in Cas9</title>
                    <journal>Nature catalysis</journal>
                    <journalAbbreviation>Nat Catal</journalAbbreviation>
                    <country></country>
                    <issue>10</issue>
                    <volume>6</volume>
                    <firstPage>969</firstPage>
                    <lastPage>977</lastPage>
                    <year>2023</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41929-023-01031-1</externalReferences>
                    <externalReferences type="pubmed">38348449</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unalinged multi-frame micrographs of AceCas9 in super-resolution, dataset 1</name>
        <directory>/data/Data_20210818_309306_slot2</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>5919</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.412</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.412</pixelHeight>
        </dimensions>
        <details>Gain file included as k3-gain-20210818.mrc.
No rotation, Flip upsidedown for motion import in RELION; no rotation, no flip in CryoSPARC.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unalinged multi-frame micrographs of AceCas9 in super-resolution, dataset 2</name>
        <directory>/data/Data_20211029_309306_AD2</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>7734</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.412</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.412</pixelHeight>
        </dimensions>
        <details>Gain file saved in dataset 1 folder as k3-gain-20210818.mrc.
No rotation, Flip upsidedown for motion import in RELION; no rotation, no flip in CryoSPARC</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
