<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-12175" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2024-06-10</depositionDate>
            <releaseDate>2025-05-22</releaseDate>
            <updateDate>2025-05-22</updateDate>
        </keyDates>
        <title>Cryo-ET dataset of dormant microsporidian spores from Encephalitozoon hellem</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-9479-3800</authorORCID>
            <firstName>Mahrukh</firstName>
            <lastName>Usmani</lastName>
            <organization type="academic">New York University</organization>
            <townOrCity>New York</townOrCity>
            <country>United States</country>
            <postOrZipCode>10016</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-0624-5020</authorORCID>
            <firstName>Bridget</firstName>
            <lastName>Carragher</lastName>
            <organization type="academic">Chan Zuckerberg Imaging Institute</organization>
            <townOrCity>Redwood City</townOrCity>
            <stateOrProvince>CA</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94065</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator private="true">
            <firstName>Clinton</firstName>
            <lastName>Potter</lastName>
            <organization type="academic">Chan Zuckerberg Imaging Institute</organization>
            <townOrCity>Redwood City</townOrCity>
            <stateOrProvince>CA</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94065</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-2570-0404</authorORCID>
            <firstName>Damian</firstName>
            <middleName>C</middleName>
            <lastName>Ekiert</lastName>
            <organization type="academic">Department of Biology, Johns Hopkins University</organization>
            <townOrCity>Baltimore</townOrCity>
            <stateOrProvince>MD</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>21218</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator private="true">
            <authorORCID>0000-0003-0624-6178</authorORCID>
            <firstName>Gira</firstName>
            <lastName>Bhabha</lastName>
            <organization type="academic">Department of Biology, Johns Hopkins University</organization>
            <townOrCity>Baltimore</townOrCity>
            <stateOrProvince>MD</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>21218</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author>Kelley K</author>
            <author authorORCID="0000-0003-0624-6178">Bhabha G</author>
            <author>Potter CS</author>
            <author authorORCID="0000-0002-0624-5020">Carragher B</author>
            <author authorORCID="0000-0001-8634-2279">Noble AJ</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</fundingBody>
                <code>F32GM128303</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>American Heart Association</fundingBody>
                <code>19POST34430065</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>Pew Biomedical Scholars</fundingBody>
                <code>PEW-00033055</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>Searle Scholars Program</fundingBody>
                <code>SSP-2018-2737</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)</fundingBody>
                <code>R01AI147131</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>Simons Foundation</fundingBody>
                <code>SF349247</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</fundingBody>
                <code>GM103310</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health (NIH)</fundingBody>
                <code>U24GM139171</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody></fundingBody>
                <code></code>
                <country></country>
            </grantReference>
        </grantSupport>
        <datasetSize units="GB">252.1</datasetSize>
        <entryDOI>10.6019/EMPIAR-12175</entryDOI>
        <experimentType>Exception</experimentType>
        <scale>cell</scale>
    </admin>
    <crossReferences>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author order="1">Kelley K</author>
                    <author authorORCID="0000-0002-8572-5857" order="2">Raczkowski AM</author>
                    <author authorORCID="0000-0003-4401-9400" order="3">Klykov O</author>
                    <author authorORCID="0000-0002-2036-232X" order="4">Jaroenlak P</author>
                    <author authorORCID="0000-0002-7388-8907" order="5">Bobe D</author>
                    <author authorORCID="0000-0003-0188-9799" order="6">Kopylov M</author>
                    <author authorORCID="0000-0002-8014-7269" order="7">Eng ET</author>
                    <author authorORCID="0000-0003-0624-6178" order="8">Bhabha G</author>
                    <author order="9">Potter CS</author>
                    <author authorORCID="0000-0002-0624-5020" order="10">Carragher B</author>
                    <author authorORCID="0000-0001-8634-2279" order="11">Noble AJ</author>
                    <author authorORCID="0000-0002-9479-3800" order="12">Usmani M</author>
                    <author order="13">Coudray N</author>
                    <author order="14">Riggi M</author>
                    <author authorORCID="0009-0006-6651-142X" order="15">Raghu R</author>
                    <author authorORCID="0009-0007-9517-3658" order="16">Ramchandani H</author>
                    <author order="17">Bobe D</author>
                    <author order="18">Kopylov M</author>
                    <author order="19">Zhong ED</author>
                    <author order="20">Iwasa JH</author>
                    <author order="21">Ekiert DC</author>
                    <title>Cryo-ET reveals the in situ architecture of the polar tube invasion apparatus from microsporidian parasites</title>
                    <journal>Proceedings of the National Academy of Sciences of the United States of America</journal>
                    <journalAbbreviation>Proc Natl Acad Sci U S A</journalAbbreviation>
                    <country></country>
                    <issue>11</issue>
                    <volume>122</volume>
                    <year>2025</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1073/pnas.2415233122</externalReferences>
                    <externalReferences type="pubmed">40067903</externalReferences>
                    <details>Currently cryo-ET data without a corresponding EMDB entry is not a supported entry data type in EMPIAR. To circumvent this issue we have referenced EMD-45674. However, this EMDB entry comes from a different dataset that we are depositing simultaneously.</details>
                </journalCitation>
            </universalCitation>
            <universalCitation>
                <journalCitation published="false" preprint="true">
                    <author authorORCID="0000-0002-9479-3800" order="1">Usmani M</author>
                    <author authorORCID="0000-0002-6050-2219" order="2">Coudray N</author>
                    <author authorORCID="0000-0002-8930-5331" order="3">Riggi M</author>
                    <author authorORCID="0009-0006-6651-142X" order="4">Raghu R</author>
                    <author order="5">Ramchandani H</author>
                    <author authorORCID="0000-0002-7388-8907" order="6">Bobe D</author>
                    <author authorORCID="0000-0003-0188-9799" order="7">Kopylov M</author>
                    <author authorORCID="0000-0001-6345-1907" order="8">Zhong ED</author>
                    <author authorORCID="0000-0002-4949-7607" order="9">Iwasa JH</author>
                    <author authorORCID="0000-0002-2570-0404" order="10">Ekiert DC</author>
                    <author authorORCID="0000-0003-0624-6178" order="11">Bhabha G</author>
                    <title>Cryo-ET reveals the in situ architecture of the polar tube invasion apparatus from microsporidian parasites</title>
                    <journal></journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country>United States</country>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Aligned tilt series from microsporidian Encephalitozoon hellem</name>
        <directory>/data/Data_Set_4</directory>
        <category>tilt series</category>
        <headerFormat>MRCS</headerFormat>
        <dataFormat>MRCS</dataFormat>
        <numImagesOrTiltSeries>12</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>SIGNED 32 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>4200</imageWidth>
            <pixelWidth>3.298</pixelWidth>
            <imageHeight>4200</imageHeight>
            <pixelHeight>3.298</pixelHeight>
        </dimensions>
        <details>Each tilt series in this dataset exists in a unique directory with an identifiable ID. Within each directory is a folder called tilt_series where the corresponding aligned tilt series and tilt file can be found.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Reconstructed tomograms from microsporidian Encephalitozoon hellem</name>
        <directory>/data/Data_Set_4</directory>
        <category>reconstructed volumes</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>12</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>variable</imageWidth>
            <pixelWidth>3.298</pixelWidth>
            <imageHeight>variable</imageHeight>
            <pixelHeight>3.298</pixelHeight>
        </dimensions>
        <details>Each reconstructed tomogram in this dataset exists in a unique directory with an identifiable ID. Within each directory is a folder called tomo_rec where the 2X binned reconstruction produced using protomo can be found.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
