<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-12257" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2024-04-23</depositionDate>
            <releaseDate>2025-04-20</releaseDate>
            <updateDate>2025-04-20</updateDate>
        </keyDates>
        <title>Cryo-EM micrographs of Vipp1-F197K/L200K helical filaments (Vipp1-F197K/L200K)</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0003-2896-1340</authorORCID>
            <firstName>Souvik</firstName>
            <lastName>Naskar</lastName>
            <organization type="academic">Imperial College London</organization>
            <street>Sir Alexander Fleming Building, Imperial College Road, South Kensington Campus</street>
            <townOrCity>London</townOrCity>
            <stateOrProvince>Greater London</stateOrProvince>
            <country>United Kingdom</country>
            <postOrZipCode>SW7 2AZ</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-1226-3217</authorORCID>
            <firstName>Harry</firstName>
            <middleName>Hamilton</middleName>
            <lastName>Low</lastName>
            <organization type="academic">Imperial College London</organization>
            <street>Sir Alexander Fleming Building, Imperial College Road, South Kensington Campus</street>
            <townOrCity>London</townOrCity>
            <stateOrProvince>Greater London</stateOrProvince>
            <country>United Kingdom</country>
            <postOrZipCode>SW7 2AZ</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-2896-1340">Naskar S</author>
            <author authorORCID="0000-0002-1226-3217">Low HH</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>Wellcome Trust</fundingBody>
                <code>215553/Z/19/Z</code>
                <country>United Kingdom</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">14.1</datasetSize>
        <entryDOI>10.6019/EMPIAR-12257</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-18319</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="false" preprint="false">
                    <author authorORCID="0000-0003-2896-1340" order="1">Naskar S</author>
                    <author order="2">Merino A</author>
                    <author order="3">Espadas J</author>
                    <author authorORCID="0000-0003-3970-7938" order="4">Singh J</author>
                    <author order="5">Roux A</author>
                    <author order="6">Colom A</author>
                    <author authorORCID="0000-0002-1226-3217" order="7">Low HH</author>
                    <title>Mechanism for Vipp1 spiral formation, ring biogenesis and membrane repair</title>
                    <journal>Nature Structure and Molecular Biology</journal>
                    <journalAbbreviation>Nat. Struct. Mol. Biol.</journalAbbreviation>
                    <country>United Kingdom</country>
                    <externalReferences type="doi">10.1038/s41594/024-01401-8</externalReferences>
                    <details>The micrographs contain 44 raw frames that accumulated 50 electrons per squared Angstrom in 4.6 seconds of exposure, without any realignment.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Raw cryo-EM micrographs of Vipp1-F197K/L200K helical filaments (Vipp1-F197K/L200K)</name>
        <directory>/data</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>30314</numImagesOrTiltSeries>
        <framesPerImage>44</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.55</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.55</pixelHeight>
        </dimensions>
        <details>The micrographs contain 44 raw frames that accumulated 50 electrons per squared Angstrom in 4.6 seconds of exposure, without any realignment. The original pixel size (0.55 Å/pix) reported above is from the super-resolution mode. However, the processing was performed after motion correction at 1.1Å/pix.</details>
        <segmentationList/>
        <micrographsFilePattern>data/GridSquare_*/Data/*_fractions.tiff</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
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