REL 2024-09-05 2024-11-20 2024-11-20 0000-0003-0608-6530 Aygul Ishemgulova Centre for Structural Biology – Central European Institute of Technology Kamenice 5 Brno Czech Republic 62500 0000-0003-4215-3315 Pavel Plevka Centre for Structural Biology – Central European Institute of Technology Kamenice 5 Brno Czech Republic 62500 Ishemgulova A Mukhamedova L Trebichalská Z Füzik T Czech Science Foundation GX19-25982X Czech Republic National Institute of Virology and Bacteriology LX22NPO5103 Czech Republic 4.2 10.6019/EMPIAR-12270 EMDB cell EMD-15710 EMD-15711 Ishemgulova A Mukhamedova L Trebichalská Z Rájecká V Payne P Šmerdová L Moravcová J Hrebík D Buchta D Škubník K Füzik T Vaňáčová Š Nováček J Plevka P Communications Biology Commun. Biol. Cryo electron tomography of Rhinovirus 2-infected cells, segmentation /data reconstructed volumes MRC MRC 1 1 32 BIT FLOAT variable variable variable variable

Tilt series: - pixel width and height: 5.532 Å - Number of tilts: 41 - number of columns, rows: 3838, 3710 - dose-symmetric tilt scheme, ± 50°, 2.5° tilt increment - rawtlt file available Tomograms: - pixel width and height: 16.60 Å (tomo7_full_rec.mrc), 22.13 Å (tomo7_full_rec_crop.mrc), tomo7_full_rec_crop.mrc used for segmentation

data/210929_HRV2_60mpi_tomo7_segmentation/210929_tomo7_Segmentation Cryo electron tomography of Rhinovirus 2-infected cos-7 cell, vitrified 60 min. post-infection. The positions of virions, empty particles, ribosomes, and microfilaments were identified using template matching as implemented in emClarity (Himes et al., 2018, Nat Methods 15). Microfilaments and membranes were segmented manually. MRC