<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-12312" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2024-03-28</depositionDate>
            <releaseDate>2025-04-24</releaseDate>
            <updateDate>2025-04-24</updateDate>
        </keyDates>
        <title>Cryo-EM micrographs for a recombinant human PNMA2 capsid</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0003-4738-9503</authorORCID>
            <firstName>Max</firstName>
            <lastName>Wilkinson</lastName>
            <organization type="academic">Broad Institute of MIT and Harvard</organization>
            <street>75 Ames St</street>
            <townOrCity>Cambridge</townOrCity>
            <stateOrProvince>Massachusetts</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>02142</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0003-0178-7995</authorORCID>
            <firstName>Feng</firstName>
            <lastName>Zhang</lastName>
            <organization type="academic">Broad Institute of MIT and Harvard</organization>
            <townOrCity>Cambridge</townOrCity>
            <country>United States</country>
            <postOrZipCode>02142</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author>Madigan V</author>
            <author authorORCID="0000-0002-1141-5821">Zhang Y</author>
            <author>Raghavan R</author>
            <author authorORCID="0000-0003-4738-9503">Wilkinson ME</author>
            <author authorORCID="0000-0001-9537-2277">Faure G</author>
            <author>Puccio E</author>
            <author>Segel M</author>
            <author>Lash B</author>
            <author authorORCID="0000-0002-7850-9056">Macrae RK</author>
            <author authorORCID="0000-0002-7444-1103">Zhang F</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>Howard Hughes Medical Institute (HHMI)</fundingBody>
                <code>NA</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)</fundingBody>
                <code>R01HG009761</code>
                <country>United States</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">1.8</datasetSize>
        <entryDOI>10.6019/EMPIAR-12312</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-42812</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author order="1">Madigan V</author>
                    <author authorORCID="0000-0002-1141-5821" order="2">Zhang Y</author>
                    <author order="3">Raghavan R</author>
                    <author authorORCID="0000-0003-4738-9503" order="4">Wilkinson ME</author>
                    <author authorORCID="0000-0001-9537-2277" order="5">Faure G</author>
                    <author order="6">Puccio E</author>
                    <author order="7">Segel M</author>
                    <author order="8">Lash B</author>
                    <author authorORCID="0000-0002-7850-9056" order="9">Macrae RK</author>
                    <author authorORCID="0000-0002-7444-1103" order="10">Zhang F</author>
                    <title>Human paraneoplastic antigen Ma2 (PNMA2) forms icosahedral capsids that can be engineered for mRNA delivery</title>
                    <journal>Proceedings of the National Academy of Sciences of the United States of America</journal>
                    <journalAbbreviation>Proc Natl Acad Sci U S A</journalAbbreviation>
                    <country></country>
                    <issue>11</issue>
                    <volume>121</volume>
                    <firstPage>e2307812120</firstPage>
                    <lastPage>308</lastPage>
                    <year>2024</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1073/pnas.2307812120</externalReferences>
                    <externalReferences type="pubmed">38437549</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame movies of recombinant PNMA2</name>
        <directory>/data/Images-Disc1</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>17600</numImagesOrTiltSeries>
        <framesPerImage>24</framesPerImage>
        <voxelType>UNSIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.6788</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.6788</pixelHeight>
        </dimensions>
        <details>Unaligned, non-gain-normalised, 24-frame movies from a Bioquantum-K3 camera recorded in super-resolution mode with twofold binning at 130,000x magnification with EPU on the MIT.nano Titan Krios G3i at 300keV. Please apply "gainref.mrc" during motion correction. This can be used as is for cryosparc, and with "Flip upside down (1)" in RELION. "particles.star" contains the final picked particles in EMD-42812</details>
        <segmentationList/>
        <micrographsFilePattern>data/Images-Disc1/GridSquare_*/Data/FoilHole_*_Data_*_*_*_*_fractions.tiff</micrographsFilePattern>
        <pickedParticlesFilePattern>data/particles.star</pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
