REL 2024-09-04 2025-01-09 2025-01-09 0000-0001-5642-3991 Jun Yu Departmemt of molecular and cellular biology, facuty of science, University of Geneva, Switzerland 30, quai Ernest-Ansermet Geneva Geneva Switzerland 1211 0000-0003-1218-6714 Andreas Boland Department of molecular and cellular biology, Faculty of Science, University of Geneva, Switzerland 30, quai Ernest-Ansermet Geneva Geneva Switzerland 1211 0000-0002-5210-2864 Miriam Stoeber Department of Cell Physiology and Metabolism, University of Geneva, Geneva, Switzerland 1 rue Michel-Servet Geneva Geneva Switzerland 1211 Yu J Kumar A Zhang X Martin C Holsbeeck KV Raia P Koehl A Laeremans T Steyaert J Manglik A Ballet S Boland A Stoeber M Swiss National Science Foundation TMSGI3_211581 Switzerland Swiss National Science Foundation 310030185235 Switzerland Swiss National Science Foundation PCEFP3_181282 Switzerland 8.0 10.6019/EMPIAR-12323 EMDB molecule EMD-18541 Yu J Kumar A Zhang X Martin C Van Holsbeeck K Raia P Koehl A Laeremans T Steyaert J Manglik A Ballet S Boland A Stoeber M Nature communications Nat Commun 1 15 2024 English 10.1038/s41467-024-52947-6 39384768 Yu J Kumar A Zhang X Martin C Raia P Koehl A Laeremans T Steyaert J Manglik A Ballet S Boland A Stoeber M bioRxiv 2023 10.1101/2023.12.06.570395 Unaligned multi-frame micrographs of the µ-opioid receptor bound to a nanobody antagonist NbE /data micrographs - multiframe MRC MRC 5939 44 1 44 UNSIGNED 16 BIT INTEGER 4096 0.9759 4096 0.9759

1. All the images are raw data. 2. All the images are multi-frame micrographs composed of 44 frames each in MRC format. 3. All the images are acquired on a Thermo Scientific Talos Arctica Cryo-TEM at an accelerating voltage of 200 kV using a Falcon III direct electron detector at a nominal magnification of 150,000x, resulting in a pixel size of 0.9759 Å. 4. Data were collected using EPU with a defocus range of −0.6 to −2.0 μm and a total electron dose of 40 e−/Å2

data/12248/data/FoilHole_*_Data_*_*_*_*_Fractions.mrc