REL 2024-10-04 2024-12-05 2024-12-05 CryoEM of E. coli RNA polymerase on lambda PR -5G to -5C promoter DNA 0000-0002-8241-3153 Seth A Darst The Rockefeller University 1230 York Avenue New York NY United States 10065 0000-0002-8241-3153 Seth A Darst The Rockefeller University 1230 York Avenue New York NY United States 10065 Saecker RM Chen J Chiu CE Malone B Sotiris J Ebrahim M Yen LY Eng ET Darst SA 1.8 10.6019/EMPIAR-12337 EMDB molecule EMD-23895 Saecker RM Chen J Chiu CE Malone B Sotiris J Ebrahim M Yen LY Eng ET Darst SA Structural origins of <i>Escherichia coli</i> RNA polymerase open promoter complex stability Proceedings of the National Academy of Sciences of the United States of America Proc Natl Acad Sci U S A 40 118 2021 English 10.1073/pnas.2112877118 34599106 Unaligned raw movies /data/tiffs2 micrographs - multiframe TIFF TIFF 4437 50 UNSIGNED BYTE 7420 1.03 7676 1.03
FEI Titan Krios + Gatan K2, super-resolution, energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 7.5 e-/ A^2/s; frame time 0.2 s (50 frames) with total exposure of 10 s; total dose 75 e-/A^2; pixel size 1.03 A/px(detector); pixel size 0.515 A/px(image); defocus range -1.1 to -2.4 um; gain reference is reference_fliprot90.mrc located in references folder.