REL
2024-06-13
2024-12-09
2024-12-09
Gelsolin-severed and capped F-actin raw data from 'Mechanism of actin filament severing and capping by gelsolin'
0000-0003-3186-5229
Roberto
Dominguez
Department of Physiology, Perelman School of Medicine, University of Pennsylvania
415 Curie Blvd.
Philadelphia
PA
United States
19104
0000-0003-3186-5229
Roberto
Dominguez
Department of Physiology, Perelman School of Medicine, University of Pennsylvania
415 Curie Blvd.
Philadelphia
PA
United States
19104
Barrie KR
Rebowski G
Dominguez R
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM073791 and GM152412
United States
13.3
10.6019/EMPIAR-12405
EMDB
molecule
EMD-43262
EMD-43263
EMD-43264
EMD-43268
EMD-43274
EMD-43316
Barrie KR
Rebowski G
Dominguez R
Mechanism of actin filament severing and capping by gelsolin
Nature structural & molecular biology
Nat Struct Mol Biol
2024
English
10.1038/s41594-024-01412-5
39448849
Dataset 1
/data/Beckman_02222023
micrographs - multiframe
TIFF
TIFF
2945
40
UNSIGNED BYTE
8184
0.54
11520
0.54
Dataset 1. Super-resolution movies (binned 2x during motion correction). Total dose = 45.6 electrons/Å^2. The gain reference is also provided as a .mrc file in data/Beckman_02222023/gainref.
data/Beckman_02222023/movies/*tiff
Dataset 2
/data/Beckman_03092023
micrographs - multiframe
TIFF
TIFF
6806
40
UNSIGNED BYTE
8184
0.54
11520
0.54
Dataset 2. Super-resolution movies (binned 2x during motion correction). Total dose = 43.7 electrons/Å^2. The gain reference is also provided as a .mrc file in data/Beckman_03092023/gainref.
data/Beckman_03092023/movies/*tiff
Dataset 3
/data/Beckman_04112023
micrographs - multiframe
TIFF
TIFF
3682
40
UNSIGNED BYTE
8184
0.54
11520
0.54
Dataset 3. Super-resolution movies (binned 2x during motion correction). Total dose = 43.7 electrons/Å^2. The gain reference is also provided as a .mrc file in data/Beckman_04112023/gainref.
data/Beckman_04112023/movies/*tiff
Dataset 4
/data/Beckman_04122023
micrographs - multiframe
TIFF
TIFF
5963
40
UNSIGNED BYTE
8184
0.54
11520
0.54
Dataset 4. Super-resolution movies (binned 2x during motion correction). Total dose = 43.7 electrons/Å^2. The gain reference is also provided as a .mrc file in data/Beckman_04122023/gainref.
data/Beckman_04122023/movies*/*tiff
Dataset 5
/data/Beckman_07022023
micrographs - multiframe
TIFF
TIFF
7814
40
UNSIGNED BYTE
8184
0.54
11520
0.54
Dataset 5. Super-resolution movies (binned 2x during motion correction). Total dose = 44 electrons/Å^2. The gain reference is also provided as a .mrc file in data/Beckman_07022023/gainref.
data/Beckman_07022023/movies*/*tiff
Mg-F-actin Severed Dataset
/data/Beckman_04052024
micrographs - multiframe
TIFF
TIFF
4594
37
UNSIGNED BYTE
8184
0.54
11520
0.54
PLEASE NOTE: This dataset was not used to generate the associated EMDs and PDBs. This dataset was collected under different sample preparations conditions (Mg-F-actin instead of Ca-G-actin) to demonstrate F-actin severing by gelsolin. The grids used for this dataset were also different (2nm ultra-thin carbon), resulting in a minor orientation bias. Please see methods and Extended Data Fig. 4 in the publication (doi.org/10.1038/s41594-024-01412-5) for more details.
Super-resolution movies (binned 2x during motion correction). Total dose = 45.3 electrons/Å^2. The gain reference is also provided as a .mrc file in data/Beckman_04052024/gainref.
data/Beckman_04052024/movies*/*tiff