Single particle reconstruction of E. coli TnsB in complex with right end fragment of Tn7 transposon

REL 2024-11-02 2025-02-03 2025-02-27 Single particle reconstruction of E. coli TnsB in complex with right end fragment of Tn7 transposon 0000-0001-5582-2851 Mariusz Czarnocki-Cieciura International Institute of Molecular and Cell Biology in Warsaw 4 Ks. Trojdena Street Warsaw Poland 02-109 0000-0001-8632-0977 Marcin Nowotny International Institute of Molecular and Cell Biology in Warsaw 4 Ks. Trojdena Street Warsaw Poland 02-109 Kaczmarska Z Czarnocki-Cieciura M Rawski M Nowotny M Foundation for Polish Science POIR.04.04.00-00-20E7/16-00 Poland National Institutes of Health (NIH) R01GM129118 United States European Union (EU) POIG.02.02.00-14-024/08-00 Poland Other government 1/SOL/2021/2 Poland 3.7 10.6019/EMPIAR-12444 EMDB molecule EMD-13439 EMD-13440 Kaczmarska Z Czarnocki-Cieciura M Górecka-Minakowska KM Wingo RJ Jackiewicz J Zajko W Poznański JT Rawski M Grant T Peters JE Nowotny M Structural basis of transposon end recognition explains central features of Tn7 transposition systems Molecular cell Mol Cell 14 82 2618 2632.e7 2022 English 10.1016/j.molcel.2022.05.005 35654042 Unaligned multi-frame movies of E. coli TnsB in complex with right end fragment of Tn7 transposon /data/Movies micrographs - multiframe TIFF TIFF 6692 40 1 40 UNSIGNED BYTE 5760 0.86 4092 0.86

Gain-corrected, unaligned multi-frame movies collected with Krios G3i microscope + K3 detector (superresolution mode, binned 2x with EPU). Voltage: 300 kV; spherical aberration: 2.7; dose per frame: 1 e/A^2 (total dose: 40.16 e/A^2 fractionated over 40 frames). Defocus range (nominal): -2.0, -1.7, -1.4, -1.1, -0.8, -0.5 µm. Objective aperture retracted, C2 aperture: 50 µm.

data/Movies/*.tiff data/TnsB_EMD-13440/EMD-13440_particlestack.star data/TnsB_EMD-13440 Aligned particles of E. coli TnsB in complex with right end fragment of Tn7 transposon (helical reconstruction) /data/TnsB_EMD-13440 picked particles - single frame - processed MRCS MRCS 450748 1 32 BIT FLOAT 512 0.86 512 0.86

Aligned particles with corresponding Relion-3 STAR file for helical reconstruction. Coordinates correspond to micrographs binned 2x during Motion correction.

data/Movies/*.tiff data/TnsB_EMD-13440/EMD-13440_particlestack.star data/TnsB_EMD-13440 Aligned particles of E. coli TnsB in complex with right end fragment of Tn7 transposon (single particle reconstruction) /data/TnsB_EMD-13439 picked particles - single frame - processed MRCS MRCS 901496 1 32 BIT FLOAT 512 0.86 512 0.86

Aligned particles with corresponding Relion-3 STAR file for single particle reconstruction. Particles were signal-subtracted; coordinates correspond to micrographs binned 2x during Motion correction.

data/Movies/*.tiff data/TnsB_EMD-13439/EMD-13439_particlestack.star data/TnsB_EMD-13439