REL 2024-09-17 2024-12-03 2024-12-03 0000-0001-5136-5331 Karolin Luger Department of Biochemistry, University of Colorado Boulder 3415 Colorado Avenue Boulder Colorado United States 80303 0000-0001-5136-5331 Karolin Luger Department of Biochemistry, University of Colorado Boulder 3415 Colorado Avenue Boulder Colorado United States 80303 Toner CM Hoitsma NM Weerawarana S Luger K 6.6 10.6019/EMPIAR-12445 EMDB molecule EMD-42053 EMD-45981 Toner CM Hoitsma NM Weerawarana S Luger K Nature communications Nat Commun 1 15 2024 English 10.1038/s41467-024-53364-5 39443461 Unaligned multi-frame micrographs of the medusavirus core particle in EER format /data/medusavirus_EMPIAR_submission/raw_micrographs_eer micrographs - multiframe EER EER 8534 1323 32 BIT FLOAT variable 0.97 variable 0.97

Raw EER files from Falcon 4 detector, acquired with EPU and a total dose of 50 e-/Å2

data/medusavirus_EMPIAR_submission/raw_micrographs_eer/*.eer Motion-corrected and dose-weighted micrographs /data/medusavirus_EMPIAR_submission/motion_corrected_micrographs micrographs - single frame MRC MRC 8534 1 32 BIT FLOAT variable 0.97 variable 0.97

Motion-corrected and dose-weighted micrographs

data/medusavirus_EMPIAR_submission/motion_corrected_micrographs/*.mrc Final particles used for reconstruction /data/medusavirus_EMPIAR_submission/final_particles picked particles - multiframe - processed MRC MRC 159734 1 16 BIT FLOAT variable 0.97 variable 0.97

Final particles used for reconstruction

data/medusavirus_EMPIAR_submission/final_particles/*.mrc CryoSPARC job of the final 3D reconstruction /data/medusavirus_EMPIAR_submission/final_reconstruction_job/J444 reconstructed volumes MRC MRC 159734 1 16 BIT FLOAT variable variable variable variable

CryoSPARC job of the final 3D reconstruction

Gain ref file /data/medusavirus_EMPIAR_submission/gain_ref micrographs - single frame MRC MRC 1 1 32 BIT FLOAT variable 0.97 variable 0.97

Gain ref file for motion correction