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        <keyDates>
            <depositionDate>2024-12-03</depositionDate>
            <releaseDate>2025-02-04</releaseDate>
            <updateDate>2025-02-04</updateDate>
        </keyDates>
        <title>single-particle cryoEM data of AsCas12f-sgRNA-DNA complex</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0001-5832-6060</authorORCID>
            <firstName>Minglei</firstName>
            <lastName>Zhao</lastName>
            <organization type="academic">Department of Biochemistry and Molecular Biology, The University of Chicago</organization>
            <street>929 E 57th Street, W201</street>
            <townOrCity>Chicago</townOrCity>
            <stateOrProvince>IL</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>60637</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-5832-6060</authorORCID>
            <firstName>Minglei</firstName>
            <lastName>Zhao</lastName>
            <organization type="academic">Department of Biochemistry and Molecular Biology, The University of Chicago</organization>
            <street>929 E 57th Street, W201</street>
            <townOrCity>Chicago</townOrCity>
            <stateOrProvince>IL</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>60637</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0001-5832-6060">Zhao M</author>
            <author authorORCID="0000-0001-9373-8458">Liu C</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</fundingBody>
                <code>R35GM143052</code>
                <country>United States</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">3.9</datasetSize>
        <entryDOI>10.6019/EMPIAR-12476</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
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    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-27801</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0001-9968-9909" order="1">Wu T</author>
                    <author authorORCID="0000-0001-9373-8458" order="2">Liu C</author>
                    <author authorORCID="0009-0005-7771-3644" order="3">Zou S</author>
                    <author order="4">Lyu R</author>
                    <author order="5">Yang B</author>
                    <author order="6">Yan H</author>
                    <author authorORCID="0000-0001-5832-6060" order="7">Zhao M</author>
                    <author authorORCID="0000-0002-5739-5416" order="8">Tang W</author>
                    <title>An engineered hypercompact CRISPR-Cas12f system with boosted gene-editing activity</title>
                    <journal>Nature chemical biology</journal>
                    <journalAbbreviation>Nat Chem Biol</journalAbbreviation>
                    <country></country>
                    <issue>11</issue>
                    <volume>19</volume>
                    <firstPage>1384</firstPage>
                    <lastPage>1393</lastPage>
                    <year>2023</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41589-023-01380-9</externalReferences>
                    <externalReferences type="pubmed">37400536</externalReferences>
                    <details>Raw micrographs collected at the Advanced Electron Microscopey Center at the University of Chicago using a Titan Krios equipped with a K3 detector</details>
                </journalCitation>
            </universalCitation>
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    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame data of AsCas12f-sgRNA-DNA complex</name>
        <directory>/data</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>7591</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.5325</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.5325</pixelHeight>
        </dimensions>
        <details>Total dose: 50 electrons per square angstrom. Gain image included as a mrc file.</details>
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        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
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