REL
2024-12-15
2026-01-05
2026-01-05
Cryo-electron tomography of neuronal synapses, Munc13-SNAP25 project
0000-0003-3698-7436
Vladan
Lucic
Max Planck Institute of Biochemistry
Am Klopferspitz 18
Martinsried
Germany
82152
0000-0003-3698-7436
Vladan
Lucic
Max Planck Institute of Biochemistry
Am Kloperspitz 18
Martinsried
Germany
82152
Papantoniou C
Lučić V
Orozco-Borunda DH
Human Frontier Science Program (HFSP)
RGP0020/2019
France
German Research Foundation (DFG)
DFG LU 1819/2-1
Germany
263.4
10.6019/EMPIAR-12512
EMDB
cell
EMD-52342
EMD-52343
Papantoniou C
Laugks U
Betzin J
Capitanio C
Ferrero JJ
Sánchez-Prieto J
Schoch S
Brose N
Baumeister W
Cooper BH
Imig C
Lučić V
Munc13- and SNAP25-dependent molecular bridges play a key role in synaptic vesicle priming
Science advances
Sci Adv
25
9
2023
English
10.1126/sciadv.adf6222
37343100
Aligned series set 1, bin-1
/data/munc13_snap25/aligned_series_bin-1
tilt series
MRC
MRCS
40
1
32 BIT FLOAT
3712
4.39
3712
4.39
The first set of aligned series, containing 40 out of 80 series. Frames were aligned using MotionCor2 and aligned based on fiducial markers using IMOD.
Aligned series set 2, bin-2
/data/munc13_snap25/aligned_series_bin-2
tilt series
MRC
MRCS
40
1
32 BIT FLOAT
1856
8.78
1856
8.78
The second set of aligned series, containing the other 40 out of 80 series. Frames were aligned using MotionCor2 and aligned based on fiducial markers using IMOD.
Tomograms bin-4
/data/munc13_snap25/tomos_bin-4
reconstructed volumes
MRC
MRC
82
1
32 BIT FLOAT
928
17.56
928
17.56
Tomograms reconstructed from the two sets of aligned series. There are 82 tomograms because there are 80 tilt series and two tilt series contain two synapses each (other series contain one synapse each). Tomograms were reconstructed by WBP (ramp filter) in IMOD. No further processing was performed (such as filtering, or denoising).