<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-12529" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2025-01-16</depositionDate>
            <releaseDate>2025-12-17</releaseDate>
            <updateDate>2025-12-17</updateDate>
        </keyDates>
        <title>Mouse RNF213 double ATPase mutant (Walker B3, Walker B4) in presence of ATP</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0003-0293-1063</authorORCID>
            <firstName>Juraj</firstName>
            <lastName>Ahel</lastName>
            <organization type="academic">IMP Vienna</organization>
            <townOrCity>Vienna</townOrCity>
            <country>Austria</country>
            <postOrZipCode>1030</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0003-1582-6924</authorORCID>
            <firstName>Tim</firstName>
            <lastName>Clausen</lastName>
            <organization type="academic">IMP Vienna</organization>
            <townOrCity>Vienna</townOrCity>
            <country>Austria</country>
            <postOrZipCode>1030</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-1582-6924">Clausen T</author>
            <author authorORCID="0000-0003-0293-1063">Ahel J</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>UK Research and Innovation (UKRI)</fundingBody>
                <code>MR/T043482/1</code>
                <country>United Kingdom</country>
            </grantReference>
            <grantReference>
                <fundingBody>European Research Council (ERC)</fundingBody>
                <code>AdG 694978</code>
                <country>Austria</country>
            </grantReference>
            <grantReference>
                <fundingBody>Biotechnology and Biological Sciences Research Council (BBSRC)</fundingBody>
                <code>BB/ P003982/1</code>
                <country>United Kingdom</country>
            </grantReference>
            <grantReference>
                <fundingBody>Austrian Research Promotion Agency</fundingBody>
                <code>852936</code>
                <country>Austria</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">1.9</datasetSize>
        <entryDOI>10.6019/EMPIAR-12529</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-52570</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="false" preprint="false">
                    <author authorORCID="0000-0003-1582-6924" order="1">Clausen T</author>
                    <author authorORCID="0000-0003-1742-6588" order="2">Fletcher AJ</author>
                    <author authorORCID="0000-0002-7052-9802" order="3">Virdee S</author>
                    <author authorORCID="0000-0003-0216-7085" order="4">Grabarczyk D</author>
                    <author authorORCID="0000-0003-0293-1063" order="5">Ahel J</author>
                    <title>ATP functions as a pathogen-associated molecular pattern to activate the E3 ubiquitin ligase RNF213</title>
                    <journal>Nature Communications</journal>
                    <journalAbbreviation>Nat. Comm.</journalAbbreviation>
                    <country>Austria</country>
                    <year>2025</year>
                    <language>English</language>
                    <details>1) aligned &amp; averaged micrographs from a data collection on a Titan Krios / Gatan K3. -1.0 to -2.2 um defocus, 31 e/A2 total exposure over 50 frames.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
        <relatedEmpiarEntries>
            <empiarEntry>EMPIAR-10712</empiarEntry>
        </relatedEmpiarEntries>
    </crossReferences>
    <imageSet>
        <name>unaligned multi-frame micrographs</name>
        <directory>/data/raw_micrographs</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>4608</numImagesOrTiltSeries>
        <framesPerImage>50</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>50</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.53</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.53</pixelHeight>
        </dimensions>
        <details>data/raw_micrographs/ == unaligned multi-frame micrographs, not gain-corrected
data/extra/201019_EM_A_GainRef.mrc == gain reference for the dataset</details>
        <segmentationList/>
        <micrographsFilePattern>data/raw_micrographs/*tiff</micrographsFilePattern>
        <pickedParticlesFilePattern>data/extra/particles.star</pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>averaged micrographs</name>
        <directory>/data/motioncorr</directory>
        <category>micrographs - single frame</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>4608</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>1.06</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>1.06</pixelHeight>
        </dimensions>
        <details>data/motioncorr/ == aligned corrected dose weighted averages via relion
data/extra/particles.star == relion 3.1+ star file describing the particles used in reconstruction of EMD-52571</details>
        <segmentationList/>
        <micrographsFilePattern>data/motioncorr/*mrc</micrographsFilePattern>
        <pickedParticlesFilePattern>data/extra/particles.star</pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
