REL
2024-11-21
2025-05-09
2025-05-09
Molecular architecture of thylakoid membranes within intact spinach chloroplasts
0000-0001-8898-2392
Wojciech
Wietrzynski
Biozentrum, University of Basel
Basel
Switzerland
4051
0000-0002-0941-4387
Benjamin
Engel
Biozentrum, University of Basel
Basel
Switzerland
4053
0000-0002-1663-0205
Matthew
Johnson
University of Sheffield, Sheffield, United Kingdom
Sheffield
United Kingdom
S10 2TN
Wietrzynski W
Johnson MP
Engel BD
Human Frontier Science Program (HFSP)
RGP0005/2021
Switzerland
303.9
10.6019/EMPIAR-12612
EMDB
cell
EMD-52542
EMD-52543
EMD-52544
EMD-52545
EMD-52546
EMD-52547
EMD-52548
Wietrzynski W
Lamm L
Wood WHJ
Loukeri MJ
Malone L
Peng T
Johnson MP
Engel BD
Molecular architecture of thylakoid membranes within intact spinach chloroplasts
bioRxiv
Switzerland
2024
10.1101/2024.11.24.625035
Spinacia oleracea, isolated chloroplast; Cryo-ET sessions; Unaligned frames and .mdocs
/data/raw_data
micrographs - multiframe
TIFF
TIFF
2028
10
SIGNED 16 BIT INTEGER
3838
3.52
3710
3.52
Directory contains raw frames and .mdoc files corresponding to 30+ tomograms acquired over multiple sessions and selected for quality. Collected on Titan G1 with Bioquantum energy filter and K2 Summit detector.
Spinacia oleracea, isolated chloroplasts; Denoised tomograms
/data/denoised_tomos
reconstructed volumes
MRC
MRC
18
1
32 BIT FLOAT
928
14.08
928
14.08
Tomograms of isolated Spinach chloroplasts denoised using CryoCARE from odd- and even-frames.
Spinacia oleracea, isolated chloroplasts; Membrane segmentations
/data/segmentations
reconstructed volumes
MRC
MRC
9
1
SIGNED BYTE
928
14.08
928
14.08
Selected segmentations of tomograms of isolated chloroplasts from Spinach. Membranes present in the volumes were detected and segmented using MemBrain-seg program.