<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-12615" schemaVersion="0.65" public="true">
    <admin>
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        <keyDates>
            <depositionDate>2025-03-04</depositionDate>
            <releaseDate>2025-06-19</releaseDate>
            <updateDate>2025-07-03</updateDate>
        </keyDates>
        <title>Cryo-EM structure of human GLUT9 apo state</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0001-7463-8398</authorORCID>
            <firstName>Tomohiro</firstName>
            <lastName>Nishizawa</lastName>
            <organization type="academic">Graduate School of Medical Life Science, Yokohama City University</organization>
            <street>1-7-29 Suehiro-cho, Tsurumi-ku</street>
            <townOrCity>Yokohama city</townOrCity>
            <stateOrProvince>Kanagawa</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>230-0045</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-7463-8398</authorORCID>
            <firstName>Tomohiro</firstName>
            <lastName>Nishizawa</lastName>
            <organization type="academic">Graduate School of Medical Life Science, Yokohama City University</organization>
            <street>1-7-29 Suehiro-cho, Tsurumi-ku</street>
            <townOrCity>Yokohama city</townOrCity>
            <stateOrProvince>Kanagawa</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>230-0045</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0009-0008-2024-2767">Matsushita D</author>
            <author authorORCID="0000-0002-1507-805X">Lee Y</author>
            <author authorORCID="0000-0001-7463-8398">Nishizawa T</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody></fundingBody>
                <code></code>
                <country></country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">4.3</datasetSize>
        <entryDOI>10.6019/EMPIAR-12615</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-60545</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author order="1">Matsushita D</author>
                    <author order="2">Toyoda Y</author>
                    <author order="3">Lee Y</author>
                    <author order="4">Aoi M</author>
                    <author order="5">Matsuo H</author>
                    <author order="6">Takada T</author>
                    <author order="7">Nishizawa T</author>
                    <title>Structural basis of urate transport by glucose transporter 9</title>
                    <journal>Cell reports</journal>
                    <journalAbbreviation>Cell Rep</journalAbbreviation>
                    <country></country>
                    <issue>4</issue>
                    <volume>44</volume>
                    <year>2025</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1016/j.celrep.2025.115514</externalReferences>
                    <externalReferences type="pubmed">40186864</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>GLUT9-apo Imageset_1 (2022/11/03)</name>
        <directory>/data/imageset_1</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>4967</numImagesOrTiltSeries>
        <framesPerImage>49</framesPerImage>
        <frameRange>
            <frameRangeMin>2</frameRangeMin>
            <frameRangeMax>49</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.83</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.83</pixelHeight>
        </dimensions>
        <details>- Microscope: FEI TITAN Krios
- Acceleration voltage (kV): 300
- Pixel size (A/pixel): 0.83
- Spherical aberration (Cs) : 2.7
- Defocus (nm): -800 to -1600
- Magnification:105000
- Detector: Gatan K3 BIOQUANTUM
- Average electron dose per image (e-/A2): 50
- Number of frames per image: 49
- Number of images: 4967
- Please use 'gain.mrc' as the gain reference with the flip along Y-axis for CryoSPARC (without flipping/rotation for RELION).
- Please omit the first frame, as it contains noise caused by an Digital Micrograph bug.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>GLUT9-apo Imageset_2 (2022/12/31)</name>
        <directory>/data/imageset_2</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>10255</numImagesOrTiltSeries>
        <framesPerImage>49</framesPerImage>
        <frameRange>
            <frameRangeMin>2</frameRangeMin>
            <frameRangeMax>49</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.83</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.83</pixelHeight>
        </dimensions>
        <details>- Microscope: FEI TITAN Krios
- Acceleration voltage (kV): 300
- Pixel size (A/pixel): 0.83
- Spherical aberration (Cs) : 2.7
- Defocus (nm): -800 to -1600
- Magnification:105000
- Detector: Gatan K3 BIOQUANTUM
- Average electron dose per image (e-/A2): 51
- Number of frames per image: 49
- Number of images: 10255
- Please use 'gain.tiff' as the gain reference without flipping/rotation.
- Please omit the first frame, as it contains noise caused by an Digital Micrograph bug.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>GLUT9-apo Imageset_3 (2023/01/04)</name>
        <directory>/data/imageset_3</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>2687</numImagesOrTiltSeries>
        <framesPerImage>48</framesPerImage>
        <frameRange>
            <frameRangeMin>2</frameRangeMin>
            <frameRangeMax>48</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.83</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.83</pixelHeight>
        </dimensions>
        <details>- Microscope: FEI TITAN Krios
- Acceleration voltage (kV): 300
- Pixel size (A/pixel): 0.83
- Spherical aberration (Cs) : 2.7
- Defocus (nm): -800 to -1600
- Magnification:105000
- Detector: Gatan K3 BIOQUANTUM
- Average electron dose per image (e-/A2): 51
- Number of frames per image: 48
- Number of images: 2687
- Please use 'gain.tiff' as the gain reference without flipping/rotation.
- Please omit the first frame, as it contains noise caused by an Digital Micrograph bug.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
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