REL
2025-04-28
2025-06-30
2025-06-30
CryoEM movies of human hemoglobin (Hb) in the presence of 5 mM sodium dithionite under Al's oil
0000-0001-6653-6682
Mark
A
Herzik, Jr
Department of Chemistry and Biochemistry, University of California, San Diego
9500 Gilman Drive
La Jolla
California
United States
92093
0000-0001-6653-6682
Mark
A
Herzik, Jr
Department of Chemistry and Biochemistry, University of California, San Diego
9500 Gilman Drive
La Jolla
California
United States
92093
Cook BD
Herzik Jr MA
National Institutes of Health (NIH)
R35-GM138206
United States
National Institutes of Health (NIH)
R01-GM148607
United States
National Institutes of Health (NIH)
1S10OD032471
United States
National Institutes of Health (NIH)
T32-EB009380
United States
1.2
10.6019/EMPIAR-12744
EMDB
molecule
EMD-45821
EMD-45822
Cook BD
Narehood SM
McGuire KL
Li Y
Akif Tezcan F
Herzik Jr MA
Preparation of oxygen-sensitive proteins for high-resolution cryoEM structure determination using blot-free vitrification
Nature communications
Nat Commun
1
16
3528
3528
2025
English
10.1038/s41467-025-58243-1
40229244
CryoEM movies of human hemoglobin (Hb) in the presence of 5 mM sodium dithionite under Al's oil. Data were collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2 with a defocus range of − 1 to − 2.5 µm. Data were collected automatically using EPU with aberration free image shift.
Unaligned multi-frame micrographs of human hemoglobin (Hb) in the presence of 5 mM sodium dithionite under Al's oil
/data/Movies
micrographs - multiframe
EER
EER
1538
1939
32 BIT FLOAT
4096
0.735
4096
0.735
Unaligned multi-frame micrographs of human hemoglobin (Hb) in the presence of 5 mM sodium dithionite under Al's oil collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2.
Aligned micrographs of human hemoglobin (Hb) in the presence of 5 mM sodium dithionite under Al's oil
/data/Micrographs
micrographs - single frame
MRC
MRC
1538
1
32 BIT FLOAT
4096
0.735
4096
0.735
Aligned micrographs of human hemoglobin (Hb) in the presence of 5 mM sodium dithionite under Al's oil collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2.
Gain file for motion correction of unaligned micrographs of human hemoglobin (Hb) in the presence of 5 mM sodium dithionite under Al's oil
/data/Gains
micrographs - single frame
MRC
MRC
1
1
32 BIT FLOAT
4096
0.735
4096
0.735
Gain file required for motion correction of unaligned micrographs of human hemoglobin (Hb) in the presence of 5 mM sodium dithionite under Al's oil.