Cryo Electron Microscopy of hIAPP and rIAPP fibrils in different aggregation solutions

REL 2025-05-15 2025-11-21 2025-11-21 Cryo Electron Microscopy of hIAPP and rIAPP fibrils in different aggregation solutions 0000-0003-1567-9514 Michal Maj Department of Chemistry - Angstrom, Uppsala University Uppsala Sweden 752 37 0000-0003-1567-9514 Michal Maj Department of Chemistry - Angstrom, Uppsala University Uppsala Sweden 752 37 Valli DV Maj MM Swedish Research Council VR 2020-05403 Sweden 737.2 10.6019/EMPIAR-12808 Exception molecule Valli DV Maj MM Cryo-Electron Microscopy Provides Mechanistic Insights into Solution-Dependent Polymorphism and Cross-Aggregation Phenomena of the Human and Rat Islet Amyloid Polypeptides Biochemistry Biochemistry 2025 English 10.1021/acs.biochem.5c00042

Subsets of particles used for 2D classification of human and rat amyloid polypeptides fibrils in different conditions. 100,000 particles from each dataset were randomly selected.

Extracted particle stacks of co-aggregates formed by human and rat IAPP (hIAPP and rIAPP) at varying ratios and in different buffer conditions. /data picked particles - multiframe - processed MRCS MRCS 1200 40 32 BIT FLOAT 4096 variable 4096 variable

This dataset contains a randomized subset of RELION-extracted particle stacks of amyloid fibrils formed by co-aggregation of human and rat islet amyloid polypeptide (hIAPP and rIAPP) under various conditions. The dataset is organized into four top-level directories based on the aggregation buffer used, and each is further subdivided into four directories corresponding to different hIAPP:rIAPP ratios (1:0, 1:1, 1:4, and 1:20).