REL
2025-06-09
2025-08-27
2025-08-27
In-situ high-resolution cryo-EM reconstructions from CEMOVIS
0000-0002-9911-706X
Johannes
Elferich
RTI, UMass Chan Medical School, Worcester, MA
368 Plantation Street
Worcester
Massachusetts
United States
01655
0000-0002-1506-909X
Nikolaus
Grigorieff
University of Massachusetts Chan Medical School
368 Plantation Street, AS4-2049
Worcester
Massachusetts
United States
01605
Elferich J
Kaminek M
Kong L
Odriozola A
Kukulski W
Zuber B
Grigorieff N
933 micrographs of CEMOVIS sections of Saccharomyces cerevisiae cells that were searched using 2D template matching, using the mature Saccharomyces cerevisiae 60S ribosomal subunit as templates. The 60S template contained omitted regions of density that was present in the reconstruction from the detected targets (EMD-71068).
Elferich J
Kaminek M
Kong L
Odriozola A
Kukulski W
Zuber B
Grigorieff N
In-situ high-resolution cryo-EM reconstructions from CEMOVIS
bioRxiv
2025
10.1101/2025.03.29.646093
307 micrographs of CEMOVIS sections of Saccharomyces cerevisiae cells that were searched using 2D template matching, using the mature Saccharomyces cerevisiae 60S ribosomal subunit as templates. The 60S template contained omitted regions of density that was present in the reconstruction from the detected targets (EMD-71068).
Unaligned multi-frame microgaphs of yeast cells sectioned using CEMOVIS
/data/micrographs
micrographs - multiframe
EER
EER
933
1863
SIGNED BYTE
16384
0.2925
16384
0.2925
Particle positions and orientations derived from 2DTM are stored in cisTEM star format.
data/2dtm_results/*.star
data/2dtm_results
Template used for 2DTM with omitted regions
/data/template
reconstructed volumes
MRC
MRC
1
1
32 BIT FLOAT
512
variable
512
variable