REL 2024-09-26 2025-10-29 2025-10-29 Electron Cryomicroscopy of alpha-Latrotoxin 0000-0002-4922-4545 Christos Gatsogiannis Center for Soft Nanoscience and Institute of Medical Physics and Biophysics Busso-Peus-Str. 10 Münster NRW Germany 48149 0000-0002-4922-4545 Christos Gatsogiannis Center for Soft Nanoscience and Institute of Medical Physics and Biophysics Busso-Peus-Str. 10 Münster NRW Germany 48149 Klink B Kalyankumar KS Gatsogiannis C German Research Foundation (DFG) SFB1348 (Project A15( to C.G.) Germany 27.8 10.6019/EMPIAR-12875 EMDB molecule EMD-51494 EMD-51465 EMD-51467 EMD-51468 EMD-51469 EMD-51472 EMD-51473 EMD-51474 EMD-51475 EMD-51492 EMD-51495 EMD-51476 EMD-51479 EMD-51485 EMD-51484 EMD-51488 EMD-51490 Klink B Alavizargar A Kalyankumar KS Chen M Heuer A Gatsogiannis C Molecular mechanism of α-latrotoxin action bioRxiv bioRxiv Germany 2024 English 10.1101/2024.03.06.583760
The EER movies are the unmodified raw movies with a total electron dose of 50 electrons per squared Angstrom.
 Klink BU Alavizargar A Kalyankumar KS Chen M Heuer A Gatsogiannis C Structural basis of α-latrotoxin transition to a cation-selective pore Nature communications Nat Commun 1 15 2024 English 10.1038/s41467-024-52635-5 39362850 EMPIAR-12308 Motion corrected micrographs of alpha-LTX /data/aLTX_mrcs_4_EMPIAR micrographs - single frame MRC MRC 87895 1 16 BIT FLOAT 4096 0.58 4096 0.58
Motion corrected micrographs of alpha-LTX. The micrographs are the motion corrected average of 49-51 movie fractions, which were themself created from 918-1225 EER movie frames. The accumulated electron dose was ~50 electrons per square Angstroem. The coordinates for the aLTX Pore and Prepore state are located in the respective folders data/aLTX_Coordinates_Pore_4_EMPIAR and data/aLTX_Coordinates_Prepore_4_EMPIAR, Picked particle positions are in corresponding .box text files (with X and Y coordinates in 1st and second column respectively). The movies in Falcon 4i Electron Event Representation (EER) are in the folder data/aLTX_EERs_4_EMPIAR.
data/aLTX_mrcs_4_EMPIAR/job*/*_EER.mrc data/aLTX_Coordinates_*_4_EMPIAR/Coordinates_*/*_EER.box data/aLTX_Coordinates_Prepore_4_EMPIAR Unaligned Falcon 4i movies in Electron Event Representation (EER) /data/aLTX_EERs_4_EMPIAR micrographs - multiframe EER EER 87915 0 BIT 16384 0.145 16384 0.145
Movies in Falcon 4i Electron Event Representation (EER).
 Particle Coordinates of the aLTX prepore state in Sphire .box format /data/aLTX_Coordinates_Prepore_4_EMPIAR picked particles - single frame - unprocessed SPIDER SPIDER 442105 1 UNSIGNED BYTE 4096 0.58 4096 0.58
Particle positions of aLTX Prepore in corresponding .box text files (with X and Y coordinates in 1st and second column respectively, as used by the software package SPHIRE).
data/aLTX_mrcs_4_EMPIAR/job*/FoilHole_* data/aLTX_Coordinates_Prepore_*/Coordinates_Prepore_*/*.box data/aLTX_Coordinates_Prepore_* Particle Coordinates of the aLTX Pore state in Sphire .box format /data/aLTX_Coordinates_Pore_4_EMPIAR picked particles - single frame - unprocessed SPIDER SPIDER 70971 1 UNSIGNED BYTE 4096 0.58 4096 0.58
Particle positions of aLTX Pore in corresponding .box text files (with X and Y coordinates in 1st and second column respectively, as used by the software package SPHIRE).
data/aLTX_mrcs_4_EMPIAR/job*/FoilHole_* data/aLTX_Coordinates_Pore_*/Coordinates_Pore_*/*.box data/aLTX_Coordinates_Pore_*