The CryoEM structure of N49-P9.6-FR3 and PGT121 Fabs in complex with BG505 SOSIP.664

REL 2025-06-09 2025-07-18 2025-07-18 The CryoEM structure of N49-P9.6-FR3 and PGT121 Fabs in complex with BG505 SOSIP.664 0000-0001-7549-9596 William David Tolbert Uniformed Services University of the Health Sciences 4301 Jones Bridge Road Bethesda Maryland United States 20814-4712 0000-0003-0594-5057 Marzena Pazgier Uniformed Services University of the Health Sciences 4301 Jones Bridge Road Bethesda Maryland United States 20814-4712 Nguyen DN Tolbert WD Pazgier M National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) R01 AI174908 United States National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) P01 AI162242 United States 702.0 10.6019/EMPIAR-12876 EMDB molecule EMD-26648 Sajadi MM Abbasi A Tehrani ZR Siska C Clark R Chi W Seaman MS Mielke D Wagh K Liu Q Jumpa T Ketchem RR Nguyen DN Tolbert WD Pierce BG Atkinson B Deming D Sprague M Asakawa A Ferrer D Dunn Y Calvillo S Yin R Guest JD Korber B Mayer BT Sato AH Ouyang X Foulke S Habibzadeh P Karimi M Aslanabadi A Hojabri M Saadat S Zareidoodeji R Kędzior M Pozharski E Heredia A Chen H Montefiori D Ferrari G Pazgier M Lewis GK Jardine JG Lusso P DeVico A A comprehensive engineering strategy improves potency and manufacturability of a near pan-neutralizing antibody against HIV Structure (London, England : 1993) Structure United States 2025 English 10.1016/j.str.2025.04.016 40373766 Grid R374_S1512 set 1 in bzip2 compressed TIFF format /data/R374_S1512-1 micrographs - multiframe TIFF TIFF 99 42 1 42 UNSIGNED BYTE variable 0.8893 variable 0.8893

Files are compressed with bzip2 Voltage = 200 GainReference = CountRef_R374_S1512_127-1_000.mrc ExposureDose = 54.9633 DoseRate = 11.4175 PixelSpacing = 0.8893 ExposureTime = 3.99867 NumSubFrames = 42 FrameDosesAndNumber = 1.3086 42

Grid R374_S1512 set 2 in bzip2 compressed TIFF format /data/R374_S1512-2 micrographs - multiframe TIFF TIFF 10 42 1 42 UNSIGNED BYTE variable 0.8893 variable 0.8893

Files are compressed with bzip2. Voltage = 200 GainReference = CountRef_R374_S1512_143-1_000.mrc ExposureDose = 54.9633 DoseRate = 9.49503 PixelSpacing = 0.8893 ExposureTime = 3.99867 NumSubFrames = 42 FrameDosesAndNumber = 1.3086 42

Grid R374_S1512 set 3 in bzip2 compressed TIFF format /data/R374_S1512-3 micrographs - multiframe TIFF TIFF 168 42 1 42 UNSIGNED BYTE variable 0.8893 variable 0.8893

Files are compressed bzip2. Voltage = 200 GainReference = CountRef_R374_S1512_153-1_000.mrc ExposureDose = 54.9633 DoseRate = 11.1836 PixelSpacing = 0.8893 ExposureTime = 3.99867 NumSubFrames = 42 FrameDosesAndNumber = 1.3086 42

Grid R374_S1512 set 4 in bzip2 compressed TIFF format /data/R374_S1512-4 micrographs - multiframe TIFF TIFF 76 42 1 42 UNSIGNED BYTE variable 0.8893 variable 0.8893

Files are compressed with bzip2. Voltage = 200 GainReference = CountRef_R374_S1512_171-1_000.mrc ExposureDose = 54.9633 DoseRate = 11.387 PixelSpacing = 0.8893 ExposureTime = 3.99867 NumSubFrames = 42 FrameDosesAndNumber = 1.3086 42

Grid R374_S1512 set 5 in bzip2 compressed TIFF format /data/R374_S1512-5 micrographs - multiframe TIFF TIFF 635 42 1 42 UNSIGNED BYTE variable 0.8893 variable 0.8893

Files are compressed with bzip2. Voltage = 200 GainReference = CountRef_R374_S1512_180-1_000.mrc ExposureDose = 54.9633 DoseRate = 11.1621 PixelSpacing = 0.8893 ExposureTime = 3.99867 NumSubFrames = 42 FrameDosesAndNumber = 1.3086 42

Grid R375_S1514 set 1 in bzip2 compressed TIFF format /data/R375_S1514-1 micrographs - multiframe TIFF TIFF 474 45 1 45 UNSIGNED BYTE variable 0.8893 variable 0.8893

Files are compressed with bzip2. Voltage = 200 GainReference = CountRef_R375_S1714_163-1_000_X-1Y-1-0.mrc ExposureDose = 58.8084 DoseRate = 11.3862 PixelSpacing = 0.8893 NumSubFrames = 45 FrameDosesAndNumber = 1.3069 45

Grid R375_S1514 set 2 in bzip2 compressed TIFF format /data/R375_S1514-2 micrographs - multiframe TIFF TIFF 1111 45 1 45 UNSIGNED BYTE variable 0.8893 variable 0.8893

Files are compressed with bzip2. Voltage = 200 GainReference = CountRef_R375_S1714_182-2_000_X-1Y-1-0.mrc ExposureDose = 58.8084 DoseRate = 11.2212 PixelSpacing = 0.8893 ExposureTime = 4.28429 NumSubFrames = 45 FrameDosesAndNumber = 1.3069 45