REL
2025-07-25
2025-12-07
2025-12-07
Single particle Cryo-EM structure of the Pdr5 drug resistance transporter
0000-0003-1144-9877
Bonaventure
Luisi
Department of Biochemistry, Sanger Building, University of Cambridge
80 Tennis Court Roa
Cambridge
Cambridgeshire
United Kingdom
CB2 1GA
0000-0003-1144-9877
Bonaventura
Luisi
Department of Biochemistry, Sanger Building, University of Cambridge
80 Tennis Court Road
Cambridge
Cambridgeshire
United Kingdom
CB2 1GA
Harris A
Wagner M
Du D
Raschka S
Nentwig LM
Gohlke H
Smits SHJ
Luisi BF
Schmitt L
Projekt DEAL
none
United Kingdom
3.8
10.6019/EMPIAR-12913
EMDB
molecule
EMD-13142
EMD-13143
EMD-13144
EMD-13145
Harris A
Wagner M
Du D
Raschka S
Nentwig LM
Gohlke H
Smits SHJ
Luisi BF
Schmitt L
Structure and efflux mechanism of the yeast pleiotropic drug resistance transporter Pdr5
Nature communications
Nat Commun
United Kingdom
1
12
2021
English
10.1038/s41467-021-25574-8
34489436
Unaligned micrographs of the Pdr5 transporter complex, separated into three separate data collections.
Unaligned multi frame micrographs of Pdr5 bound to ADP.
/data/Pdr5_03aug20_Micrographs
micrographs - multiframe
TIFF
TIFF
3415
49
UNSIGNED BYTE
variable
variable
variable
variable
Pdr5 sample with added ATP yielded a map containing the hydrolysed nucleotide ADP in the canonical and ATP in the inactive site, referred to in the paper as ADP-Pdr5.
data/Pdr5_03aug20_Micrographs
Unaligned multi frame micrographs of Pdr5 bound to R6G.
/data/Pdr5_R6G_11aug20_Micrographs
micrographs - multiframe
TIFF
TIFF
3604
49
UNSIGNED BYTE
variable
variable
variable
variable
Pdr5 with added ATP and transport substrate rhodamine 6 G (R6G).
data/Pdr5_R6G_11aug20_Micrographs
Unaligned multi frame micrographs of Pdr5 bound to ADP.
/data/Pdr5_Vi_14aug20_Micrographs
micrographs - multiframe
TIFF
TIFF
8370
49
UNSIGNED BYTE
variable
variable
variable
variable
Pdr5 with added ATP and sodium orthovanadate (Vi).
data/Pdr5_Vi_14aug20_Micrographs