REL 2025-09-08 2025-11-30 2025-11-30 Cryo-EM micrographs of R2Tg retrotransposon initiating target-primed reverse transcription 0000-0003-4738-9503 Max Wilkinson Massachusetts Institute of Technology 75 Ames Street Cambridge Massachusetts United States 02142 0000-0003-0178-7995 Feng Zhang Broad Institute of Harvard and MIT 75 Ames Street Cambridge Massachusetts United States 02142 Wilkinson ME Zhang F 1.7 10.6019/EMPIAR-12960 EMDB molecule EMD-47091 Edmonds KK Wilkinson ME Strebinger D Chen H Lash B Schaefer CC Zhu S Liu D Zilberzwige-Tal S Ladha A Walsh ML Frangieh CJ Vaz Reay NA Macrae RK Wang X Zhang F Structure and biochemistry-guided engineering of an all-RNA system for DNA insertion with R2 retrotransposons Nature communications Nat Commun United States 1 16 2025 English 10.1038/s41467-025-61321-z 40603868 Unaligned multi-frame movies of Taeniopygia guttata R2 retrotransposon initiating target-primed reverse transcription /data/Krios_20240308_TgD4 micrographs - multiframe TIFF TIFF 10628 40 UNSIGNED BYTE 5760 0.663 4092 0.663
RAW MICROGRAPHS AND REFINEMENT STAR FILE FOR R2Tg TPRT COMPLEX (EMD-47091; PDB 9DOU) Data collected at MIT.nano on Thermo Scientific Titan Krios G3i cryo-TEM Voltage 300 keV Camera Gatan K3, super-resolution mode with 2-fold binning by EPU Energy filter GIF, 20eV slit width Magnification 130000 Pixel size 0.663 A/pix Dose 59.6 e/A2 Exposure rate 25.7 e/pix/s Exposure time 1.02 s Fractions 40 Total mics 10628 GainReference.mrc was binned using 'rebin by two' in Digital Micrograph. It needs to be flipped for use in RELION motion correction (Gain flip: Flip upside down (1) ). It can probably be used as is for cryoSPARC. particles.star contains metadata for the particle selected for the final reconstruction.
data/Krios_20240308_TgD4/Images-Disc1/GridSquare_*/Data/FoilHole_*_fractions.tiff data/particles.star