REL
2025-08-27
2025-12-07
2025-12-07
Cryo electron tomography of wild-type Drosophila nephrocytes
0000-0002-8067-6611
Achilleas
S.
Frangakis
Buchmann Institute for Molecular Life Sciences and Institute of Biophysics, Goethe University Frankfurt
Max-von-Laue Str. 15
Frankfurt am Main
Hessen
Germany
60438
0000-0002-8067-6611
Achilleas
S.
Frangakis
Buchmann Institute for Molecular Life Sciences and Institute of Biophysics, Goethe University Frankfurt
Frankfurt am Main
Germany
60438
0000-0002-0441-7749
Tobias
Hermle
Renal Division, Department of Medicine, Faculty of Medicine and Medical Center - University of Freiburg
Breisacher Str. 113
Freiburg
Baden-Württemberg
Germany
79106
Moser D
Lang K
Birtasu AN
Grahammer F
Helmstaedter M
Scheffer MP
Hermle T
Frangakis AF
German Research Foundation (DFG)
GRK 2566/1, CRC 1192, GR3933/1-1, TRR- 422, FR 1653/14-1, HE 7456/7-1, SFB 1453/431984000, HE 7456/6-1
Germany
1.5
10.6019/EMPIAR-13009
EMDB
cell
EMD-53557
Moser D
Lang K
Birtasu AN
Grahammer F
Helmstaedter M
Scheffer MP
Hermle T
Frangakis AF
The slit diaphragm in Drosophila exhibits a bilayered, fishnet architecture
10.1038/s41467-025-64347-5
Unaligned multi-frame micrographs of wild-type nephrocytes displaying slit diaphragms
/data/frames
micrographs - multiframe
MRC
MRC
52
17
16
19
SIGNED BYTE
11520
1.339
8184
1.339
Unaligned multi-frame micrographs acquired in super-resolution mode (pixel size 1.34A), with 41-58 micrographs per tilt-series. The corresponding .mdoc files containing the metadata for each tilt-series are in the subdirecory "mdoc", following the naming tomoXX.mrc.mdoc, with XX corresponding to the tilt-series ID.
Unaligned tilt-series and alignment metadata
/data/tilt_series_alignment
tilt series
MRC
MRC
17
1
32 BIT FLOAT
5760
2.679
4092
2.679
Unaligned tilt-series (tomoXX.st) and IMOD's etomo alignment metadata for each tilt-series (tomoXX.xf, tomoXX.tlt). XX corresponds to the tilt-series ID.
Reconstructed tomograms of wild-type nephrocytes displaying slit diaphragms
/data/tomograms
reconstructed volumes
MRC
MRC
17
1
32 BIT FLOAT
1024
10.72
1440
10.72
Reconstructed volumes of wild-type nephrocytes displaying slit diaphragms (rec_tomoXX.mrc, XX corresponds to tilt-series IDs).