REL
2025-10-06
2025-10-29
2025-10-29
Systematic membrane thickness variation across cellular organelles revealed by cryo-ET
0000-0002-7397-1321
Martin
Beck
Max Planck Institute of Biophysics
Max-von-Laue-Straße 3
Frankfurt am Main
Hessen
Germany
60438
0000-0002-7397-1321
Martin
Beck
Max Planck Institute of Biophysics
Max-von-Laue-Straße 3
Frankfurt am Main
Hessen
Germany
60438
Glushkova D
Boehm S
Beck M
Max Planck Society
NA
Germany
97.9
10.6019/EMPIAR-13031
EMDB
cell
EMD-55308
EMD-55310
Glushkova D
Boehm S
Beck M
Systematic membrane thickness variation across cellular organelles revealed by cryo-ET
Journal of Cell Biology
JCB
cryoCARE-denoised bin4 tomograms of HEK293 cells
/data/cryoCARE
reconstructed volumes
MRC
MRC
15
1
32 BIT FLOAT
1024
7.884
1024
7.884
CryoCARE denoised bin4 tomograms of HEK293 cells. Denoising was performed on even and odd frames. Tomograms were reconstructed using weighted back-projection (WBP) implemented in AreTomo2.
Processed tilt series with alignment files for HEK293 cells
/data/TiltSeries
tilt series
MRC
MRC
15
1
SIGNED 16 BIT INTEGER
4096
1.971
4096
1.971
Aligned, sorted, and cleaned tilt series (prior to and after dose-filtering), organized in separate folders. Each tilt series folder includes acquisition metadata (.mrc.mdoc), tilt angles (.rawtlt), and GCTF CTF estimation output (.star) files. An AreTomo subfolder within each tilt series contains AreTomo2 alignment (.aln) and IMOD transform (.xf) files used for weighted back-projection reconstruction.