REL 2025-09-18 2025-11-16 2025-11-16 0000-0002-9373-4737 Sebastian Klinge Laboratory of Protein and Nucleic Acid Chemistry, Rockefeller University 1230 York Avenue New York New York United States 10065 0000-0002-9373-4737 Sebastian Klinge Laboratory of Protein and Nucleic Acid Chemistry, Rockefeller University New York United States 10065 Buzovetsky OB National Institutes of Health (NIH) R01GM145950 United States National Institutes of Health (NIH) R35GM156426 United States Chan Zuckerberg Initiative Exploratory Cell Network United States 187.0 10.6019/EMPIAR-13047 EMDB molecule EMD-49075 EMD-49076 EMD-49077 EMD-49078 EMD-49079 EMD-49080 EMD-49082 EMD-49083 EMD-49084 EMD-49085 EMD-49086 EMD-49087 EMD-49088 EMD-49089 EMD-49090 EMD-49091 Buzovetsky OB Klinge SK Nature Nature english

Raw micrographs

Unaligned multi-frame micrographs of SSU processome assembly intermediates - Dataset1 Kre33 pulldown /data/Dataset1_Kre33pulldown_tiffs micrographs - multiframe TIFF TIFF 44272 40 UNSIGNED BYTE 11520 0.54 8184 0.54

The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -1 to -2.5 µm, and a total dose of 60.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.

Unaligned multi-frame micrographs of yeast SSU processome assembly intermediates - Dataset2 part1 Dhr1 pulldown /data/Dataset2_part1_Dhr1pulldown_tiffs micrographs - multiframe TIFF TIFF 36229 40 UNSIGNED BYTE 11520 0.54 8184 0.54

The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -1 to -2.5 µm, and a total dose of 61.6 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.

Unaligned multi-frame micrographs of yeast SSU processome assembly intermediates - Dataset2 part2 Dhr1 pulldown /data/Dataset2_part2_Dhr1pulldown_tiffs micrographs - multiframe TIFF TIFF 48234 40 UNSIGNED BYTE 11520 0.54 8184 0.54

The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -1 to -2.5 µm, and a total dose of 61.6 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.

Unaligned multi-frame micrographs of yeast SSU processome assembly intermediates - Dataset3 - Upt14 pulldown /data/Dataset3_Upt14pulldown_tiffs micrographs - multiframe TIFF TIFF 85031 40 UNSIGNED BYTE 11520 0.54 8184 0.54

The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -1 to -2.5 µm, and a total dose of 61.6 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.

Polished particle stacks of yeast SSU processome assembly intermediates- State A to State O /data/Particle_stacks micrographs - single frame MRCS MRCS 801888 40 32 BIT FLOAT 480 1.063 480 1.063

Particles stacks from the four datasets for each state (State A to State O). Particles are polished, magnification anisotropy corrected and per-particle defocus corrected. A Relion .star format file is provided and contains the final Euler angles, shifts and also the micrograph coordinates.