REL 2025-10-10 2025-12-12 2025-12-12 0000-0002-0634-538X Peter Cherepanov The Francis Crick Institute 1 Midland Road London London United Kingdom NW1 1AT 0000-0002-0634-538X Peter Cherepanov The Francis Crick Institute 1 Midland Road London London United Kingdom NW1 1AT Nans A Chenavier F Hope J Zhang P Cherepanov P National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) U54AI170791 United Kingdom Wellcome Trust CC2058 United Kingdom Medical Research Council (MRC, United Kingdom) CC2058 United Kingdom Cancer Research UK CC2058 United Kingdom 11.0 10.6019/EMPIAR-13078 EMDB virus EMD-55409 Singer RM Zhen L Rey SJ Hope J Chenavier F Cook JN Punch E Smith J Zhou Z Maslen S Masino L Nans A Skehel M Taylor AI Zanetti G Zhang P Perilla RJ Engelman NA Cherepanov P Nans A English

The data represent 14 tilt series, each from −60° to +60° with 3° increments, recorded using Krios G2 microscope with Falcon 4i detector and a Selectris energy filter. The data was acquired using the dose-symmetric scheme, a total exposure of 104 e/Å2 (2.5 e/Å2 per tilt), a calibrated magnification resulting in a pixel size of 1.56 Å, and a defocus range of -2.5 to -5.5 µm. Each tilt micrograph movie contains 6 frames in TIFF format. The calibrated tilt axis is 95 degrees.

Unaligned multi-frame micrographs from tilt series of HIV-1 cores, associated mdoc files, and gain reference /data tilt series TIFF TIFF 14 6 UNSIGNED BYTE 4096 1.56 4096 1.56

Each tilt image is comprised of 6 unaligned raw movie frames in TIFF format without gain correction. Gain reference is supplied as a separate file. Dose per tilt image is 2.54 e/A2 and total dose for each tilt series is 104 e/A2. Tilt azimuth is is 95 degrees.