REL
2025-10-14
2025-12-05
2025-12-05
CryoEM of mycobacterial open-gate proteasome complexed with engineered bacterial enhancer binding protein (bEBP)
0000-0002-8241-3153
Seth
Darst
The Rockefeller University
1230 York Avenue
New York
New York
United States
10065
0000-0002-8241-3153
Seth
Darst
The Rockefeller University
1230 York Avenue
New York
New York
United States
10065
Mueller AU
Molina N
Nixon BT
Darst SA
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R35 GM118130
United States
479.4
10.6019/EMPIAR-13093
EMDB
molecule
EMD-48574
Mueller AU
Molina N
Nixon BT
Darst SA
Real-time capture of σN transcription initiation intermediates reveals mechanism of ATPase-driven activation by limited unfolding
Nature communications
Nat Commun
1
16
2025
English
10.1038/s41467-025-61837-4
40759887
EMPIAR-13091
Unaligned multi-frame micrographs of an engineered bEBP-proteasome complex (0 degree tilt)
/data/rawdata_0deg
micrographs - multiframe
TIFF
TIFF
503
40
UNSIGNED BYTE
3710
1.5
3838
1.5
Raw micrographs were collected on a Talos Arctica at 200kV equipped with a Gatan K2 camera. Recordings were performed in counting mode at a pixel size of 1.5 A/px with a dose rate of 8.4 electrons/px/s for an exposure time of 12 seconds in dose-fraction mode of 0.3 seconds per-frame resulting in a total of 40 frames and 1.12 electrons/A^2/frame (total dose of 44.8 electrons/A^2). Defocus range for the collection was -0.8 to -3.0 microns. The tilt angle of the specimen was 0 degrees.
data/rawdata_0deg/AM51-4_*.tif
data/particle_stacks/*.star
data/particle_images_0deg_J71
Unaligned multi-frame micrographs of an engineered bEBP-proteasome complex (20 degree tilt)
/data/rawdata_20deg
micrographs - multiframe
TIFF
TIFF
466
40
UNSIGNED BYTE
3710
1.5
3838
1.5
Raw micrographs were collected on a Talos Arctica at 200kV equipped with a Gatan K2 camera. Recordings were performed in counting mode at a pixel size of 1.5 A/px with a dose rate of 9.2 electrons/px/s for an exposure time of 12 seconds in dose-fraction mode of 0.3 seconds per-frame resulting in a total of 40 frames and 1.227 electrons/A^2/frame (total dose of 49.1 electrons/A^2). Defocus range for the collection was -1 to -2.5 microns. The tilt angle of the specimen was 20 degrees.
data/rawdata_20deg/AM52-2_*.tif
data/particle_stacks/*.star
data/particle_images_20deg_J66
Aligned single-frame micrographs of an engineered bEBP-proteasome complex (0 degree tilt)
/data/motioncor_0deg/job002/tiffs
micrographs - single frame
MRC
MRC
503
1
UNSIGNED BYTE
3710
1.5
3838
1.5
0 degree tilt data. Raw movies of were aligned using MotionCor2 (through Relion) with a binning factor of 1 and a dose of 1.12 electrons/A^2/frame. Full job output is provided in the upstream directory.
data/motioncor_0deg/job002/tiffs/AM51-4_*.mrc
data/particle_stacks/*.star
data/particle_images_0deg_J71
Aligned single-frame micrographs of an engineered bEBP-proteasome complex (20 degree tilt)
/data/motioncor_20deg/job002/tiffs
micrographs - single frame
MRC
MRC
466
1
UNSIGNED BYTE
3710
1.5
3838
1.5
20 degree tilt data. Raw movies of were aligned using MotionCor2 (through Relion) with a binning factor of 1 and a dose of 1.227 electrons/A^2/frame. Full job output is provided in the upstream directory.
data/motioncor_20deg/job002/tiffs/AM52-2_*.mrc
data/particle_stacks/*.star
data/particle_images_20deg_J66
Particle images of an engineered bEBP-proteasome complex (0 degree tilt)
/data/particle_images_0deg_J71
picked particles - single frame - processed
MRC
MRC
308092
0
UNSIGNED BYTE
288
1.5
288
1.5
0 degree tilt data. Number of frames per file is variable (0 was chosen as placeholder). Number of images indicates the total number of particle images across all files.
data/particle_images_0deg_J71/*_AM51-4_*_particles.mrc
data/particle_stacks/*.star
data/particle_images_0deg_J71
Particle images of an engineered bEBP-proteasome complex (20 degree tilt)
/data/particle_images_20deg_J66
picked particles - single frame - processed
MRC
MRC
283067
0
UNSIGNED BYTE
288
1.5
288
1.5
20 degree tilt data. Number of frames per file is variable (0 was chosen as placeholder). Number of images indicates the total number of particle images across all files.
data/particle_images_20deg_J66/*_AM52-2_*_particles.mrc
data/particle_stacks/*.star
data/particle_images_20deg_J66