REL
2025-09-01
2026-03-20
2026-03-20
Single particle cryo-EM dataset of as-prepared Bos taurus mitochondrial complex I in MSP2N2 nanodiscs treated with NADH
0000-0001-8667-6797
Judy
Hirst
MRC MBU, University of Cambridge
Hills Road
Cambridge
Cambridgeshire
United Kingdom
CB2 0XY
0000-0001-8667-6797
Judy
Hirst
MRC MBU, University of Cambridge
Hills Road
Cambridge
United Kingdom
CB2 0XY
Chung I
Hirst J
Medical Research Council (MRC, United Kingdom)
MC_UU_00015/2
United Kingdom
Medical Research Council (MRC, United Kingdom)
MC_UU_00028/1
United Kingdom
Sao Paulo Research Foundation (FAPESP)
2023/00934-5
Brazil
Sao Paulo Research Foundation (FAPESP)
2020/14542-3
Brazil
5.2
10.6019/EMPIAR-13115
EMDB
molecule
EMD-55030
EMD-55031
EMD-55032
EMD-55033
EMD-55034
Chung I
Pereira CS
Wright JJ
Arantes GM
Hirst J
Post-catalysis structures of mitochondrial complex I with ubiquinol-10 bound in the active site
Nature communications
Nat Commun
2026
English
10.1038/s41467-026-70030-0
41786699
Unaligned multi-frame micrographs of Bos taurus mitochondrial complex I from an as-prepared sample solubilised in DDM and reconstituted in to MSP2N2 nanodiscs, with 10 micromolar NADH added.
/data
micrographs - multiframe
TIFF
TIFF
13056
40
1
40
UNSIGNED BYTE
11520
variable
8184
variable
Unaligned multi-frame micrographs of Bos taurus mitochondrial complex I from an as-prepared sample solubilised in DDM and reconstituted in to MSP2N2 nanodiscs. Images (TIFF) were collected with a 300 keV FEI Titan Krios on a Falcon K3 detector, using counting mode with super-resolution mode and a Gatan energy filter (20 eV). The C2 and objective lens apertures were set to 70 and 100 µm, respectively. 2.14 s exposures were captured at a dose rate of 18.8 electrons Å−2 s−1 in 40 frames with a total dose of 40.29 electrons Å−2. A pixel size of 0.536 Å/pixel (super resolution; nominal pixel size was 1.072 Å/pixel) was used.
data/Raw_data/GridSquare*/Data/*tiff