<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-13163" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2025-12-11</depositionDate>
            <releaseDate>2026-04-16</releaseDate>
            <updateDate>2026-04-16</updateDate>
        </keyDates>
        <title>PglZ in complex with BrxB-BrxC fusion from the Acinetobacter BREX system</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-0008-473X</authorORCID>
            <firstName>Lindsey</firstName>
            <lastName>Doyle</lastName>
            <organization type="academic">Fred Hutch Cancer Center</organization>
            <street>1100 Fairview Ave N</street>
            <townOrCity>Seattle</townOrCity>
            <stateOrProvince>Washington</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>98109</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0003-0339-0035</authorORCID>
            <firstName>Brett</firstName>
            <middleName>K</middleName>
            <lastName>Kaiser</lastName>
            <organization type="academic">Department of Biology, Seattle University</organization>
            <street>901 12th Ave</street>
            <townOrCity>Seattle</townOrCity>
            <stateOrProvince>WA</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>98122</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-5174-7858</authorORCID>
            <firstName>Barry</firstName>
            <middleName>L</middleName>
            <lastName>Stoddard</lastName>
            <organization type="academic">Division of Basic Sciences, Fred Hutchinson Cancer Center</organization>
            <street>1100 Fairview Ave N</street>
            <townOrCity>Seattle</townOrCity>
            <stateOrProvince>WA</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>98109</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-0008-473X">Doyle LA</author>
            <author authorORCID="0000-0001-5174-7858">Stoddard BL</author>
            <author authorORCID="0000-0003-0339-0035">Kaiser BK</author>
            <author authorORCID="0009-0001-3643-1721">Kaiser AJ</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</fundingBody>
                <code>R35 GM148166</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</fundingBody>
                <code>R15 GM140375</code>
                <country>United States</country>
            </grantReference>
            <grantReference>
                <fundingBody>Other private</fundingBody>
                <code>N/A</code>
                <country>United States</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">4.2</datasetSize>
        <entryDOI>10.6019/EMPIAR-13163</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-74400</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="false" preprint="false">
                    <author authorORCID="0000-0002-0008-473X" order="1">Doyle LD</author>
                    <title>Competing forms of protein-protein association and DNA binding exhibited by BrxC from the BREX phage restriction system</title>
                    <journal></journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country>United States</country>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Motion Corrected micrographs for the BrxC-BrxB/PglZ complex</name>
        <directory>/data/Averaged</directory>
        <category>micrographs - single frame</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>3964</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>1.122</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>1.122</pixelHeight>
        </dimensions>
        <details>Micrographs are averages motion corrected by WARP, binning pixels from 0.561 to 1.122 Å/px. Voltage 200 kV, Cs 2.7, Total exposure dose 50. Prior to motion correction, the gain was corrected.</details>
        <segmentationList/>
        <micrographsFilePattern>data/Averaged/20240530_LD02-1_*.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned multi-frame micrographs of the BrxC-BrxB/PglZ complex</name>
        <directory>/data/RawData</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>3964</numImagesOrTiltSeries>
        <framesPerImage>100</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>100</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.561</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.561</pixelHeight>
        </dimensions>
        <details>The data is raw, multi-frame movies without any correction applied. Data was collected in SerialEM as LZW compressed TIFFs.</details>
        <segmentationList/>
        <micrographsFilePattern>data/RawData/20240530_LD02-1_*.tif</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
