<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-13259" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2025-12-30</depositionDate>
            <releaseDate>2026-07-01</releaseDate>
            <updateDate>2026-07-01</updateDate>
        </keyDates>
        <title>Echovirus 18 genome release in situ, SPA</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-7294-6714</authorORCID>
            <firstName>Liya</firstName>
            <middleName>Nailevna</middleName>
            <lastName>Mukhamedova</lastName>
            <organization type="academic">CEITEC Masaryk University</organization>
            <street>Kamenice 5</street>
            <townOrCity>Brno</townOrCity>
            <stateOrProvince>South Moravia</stateOrProvince>
            <country>Czech Republic</country>
            <postOrZipCode>625 00</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0003-4215-3315</authorORCID>
            <firstName>Pavel</firstName>
            <lastName>Plevka</lastName>
            <organization type="academic">CEITEC, Masaryk University</organization>
            <street>Kamenice, 5</street>
            <townOrCity>Brno</townOrCity>
            <stateOrProvince>South Moravia</stateOrProvince>
            <country>Czech Republic</country>
            <postOrZipCode>62500</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-7294-6714">Mukhamedova LN</author>
            <author authorORCID="0000-0003-4215-3315">Plevka P</author>
            <author authorORCID="0000-0003-1710-1628">Andersen TJ</author>
            <author authorORCID="0000-0002-8534-4915">Trebichalska Z</author>
            <author authorORCID="0000-0003-4013-3898">Novacek J</author>
            <author authorORCID="0000-0001-6787-6355">Buchta D</author>
            <author authorORCID="0000-0003-0708-0771">Levdansky Y</author>
            <author authorORCID="0000-0002-9146-6505">Moravcova J</author>
            <author authorORCID="0000-0003-4568-0196">Hrebik D</author>
            <author authorORCID="0000-0002-1190-0210">Fuzik T</author>
            <author authorORCID="0000-0002-1719-7677">Tollefsrud TG</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>European Research Council (ERC)</fundingBody>
                <code>101043452</code>
                <country>Czech Republic</country>
            </grantReference>
            <grantReference>
                <fundingBody>European Union (EU)</fundingBody>
                <code>CZ.02.01.01/00/22_008/0004607</code>
                <country>Czech Republic</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="GB">540.0</datasetSize>
        <entryDOI>10.6019/EMPIAR-13259</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>virus</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-54484</emdbEntry>
            <emdbEntry>EMD-54622</emdbEntry>
            <emdbEntry>EMD-54764</emdbEntry>
            <emdbEntry>EMD-55096</emdbEntry>
            <emdbEntry>EMD-54510</emdbEntry>
            <emdbEntry>EMD-54506</emdbEntry>
            <emdbEntry>EMD-54765</emdbEntry>
            <emdbEntry>EMD-54763</emdbEntry>
            <emdbEntry>EMD-55863</emdbEntry>
            <emdbEntry>EMD-55864</emdbEntry>
            <emdbEntry>EMD-55865</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="false" preprint="false">
                    <author authorORCID="0000-0002-7294-6714" order="1">Mukhamedova LN</author>
                    <author authorORCID="0000-0003-4215-3315" order="2">Plevka P</author>
                    <title>Particles of echovirus 18 open to release their genomes in vivo</title>
                    <journal></journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country>Czech Republic</country>
                    <year>2026</year>
                    <language>English</language>
                    <details>Total dose: 23.6 e/px/s
Number of frames: 5
Exposure time: 8 s</details>
                </journalCitation>
            </universalCitation>
        </citationList>
        <relatedEmpiarEntries>
            <empiarEntry>EMPIAR-47487237</empiarEntry>
        </relatedEmpiarEntries>
    </crossReferences>
    <imageSet>
        <name>E18 particles releasing genome in vivo micrographs</name>
        <directory>/data/Micrographs</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>1290</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>40</frameRangeMax>
        </frameRange>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>2.08047</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>2.08047</pixelHeight>
        </dimensions>
        <details>Micrographs are motion-corrected (MotionCor2) and ctf-estimated (Ctffind 3). Particles coordinates are extracted from Cryosparc in .cs format. Dose: 4.19 e/A^2/s, exposure: 8s.</details>
        <segmentationList/>
        <micrographsFilePattern>data/Micrographs/mic_*.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern>data/Coordinates/particles_selected.cs</pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>E18 particles releasing genome in vivo movies</name>
        <directory>/data/Movies</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>1290</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>40</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>2.08047</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>2.08047</pixelHeight>
        </dimensions>
        <details>Raw data, not preprocessed. Dose: 4.19 e/A^2/s, exposure: 8s.</details>
        <segmentationList/>
        <micrographsFilePattern>data/Movies</micrographsFilePattern>
        <pickedParticlesFilePattern>data/Coordinates/particles_selected.cs</pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
