REL 2026-02-08 2026-03-23 2026-03-23 Cryo electron tomography of yeast FIB-milled lamella after sublimation 0000-0002-1890-5524 Genís Valentín Gesé Karolinska Institutet Solnavagen 9 Solna Sweden 17165 0000-0002-1890-5524 B. Martin Hallberg Karolinska Institutet Solnavagen 9 Solna Sweden 17165 Bondy AL Valentin Gese G Thersleff T Hallberg BM 857.7 10.6019/EMPIAR-13298 FIB-SEM cell Bondy AL Valentin Gese G Thersleff T Hallberg BM In-Chamber Sublimation: A Hands-On Hack to Beat Ice and Curtaining in Cryo-Electron Tomography Lamella Prep Unaligned multi-frame micrographs of FIB-milled yeast lamella /data/movies micrographs - multiframe MRC MRC 657 6 UNSIGNED BYTE 11520 1.34 8184 1.34
MDOC files for each tilt-series are in the folder "MDOC". Movie averages after WARP 1.0.9 processing (bin 1, pixel size 2.68) are in the folder "average". Aretomo-reconstructed tomograms (10.72 A/px), tilt-series alignments (*.xf) and other aretomo output files are in the folder "aretomo". WARP-reconstructed tomograms (16.08 A/px), as well as denoised tomograms (WARP noise2map) are in the folder reconstruction. Subtomograms exported by M, as well as CTF files, are in the folder "subtomo_EMPIAR". The corresponding particle coordinates and poses after RELION 3.1 refinement are in the file run_data.star.