REL 2025-12-09 2026-05-31 2026-05-31 0000-0001-9470-0347 Katja Petzold Department of Medical Biochemistry and Microbiology, Uppsala University Husargatan 3 Uppsala Sweden 752 37 0000-0001-9470-0347 Katja Petzold Department of Medical Biochemistry and Microbiology, Uppsala University Husargatan 3 Uppsala Sweden 752 37 Steinmetzger C Riad M Petzold K European Molecular Biology Organization (EMBO) ALTF 1011-2020 European Union H2020 Marie Curie Actions of the European Commission 747446 European Union The Swedish Foundation for Strategic Research ICA14-0023 Sweden Swedish Research Council 2014-04303 Sweden Swedish Research Council 2018-00250 Sweden Ake Wiberg Foundation 467080968 Sweden Ake Wiberg Foundation M14-0109 Sweden Other private JS20140009 Sweden Carl Trygger Foundation CTS14-383 Sweden Carl Trygger Foundation CTS15-383 Sweden Other private M91/14 Sweden 16.6 10.6019/EMPIAR-13345 EMDB molecule EMD-56070 EMD-56091 EMD-56128 EMD-56827 EMD-56828 EMD-56829 EMD-56830 EMD-56831 EMD-56832 EMD-56833 EMD-56834 EMD-56835 EMD-56836 EMD-56837 EMD-56839 EMD-56840 EMD-56841 EMD-56842 EMD-56843 EMD-56844 EMD-56845 EMD-56846 EMD-56847 EMD-56848 EMD-56849 EMD-56850 EMD-56851 EMD-56852 EMD-56853 EMD-56854 Steinmetzger C Karlsson H Fontana C Galindo Riera N Riad M Kosek D Schlagnitweit J Steiner E Sandoz SF Andersson ER Petzold K bioRxiv 2026 10.64898/2026.05.08.718354 Unaligned multi-frame micrographs of E. coli 70S ribosome /data/wt/movies micrographs - multiframe TIFF TIFF 15518 50 UNSIGNED BYTE 5760 0.505 4092 0.505

Movies were collected using a Titan Krios G3i microscope (Thermo Fisher Scientific) operating at 300 kV equipped with a K3 BioQuantum detector (Gatan) at the Karolinska Institutet 3D-EM facility in Solna, Sweden. The data was acquired using EPU software (Thermo Fisher Scientific) with a pixel size of 0.505 Å/px (nominal magnification 165000x), electron dose of ~50 e−/Å2, 50 frames per movie, and a defocus range of −0.3 to −2.0 µm. The movies are gain-corrected.

data/wt/movies/*.tiff PDB 9TMI / EMD-56070: E. coli 70S ribosome with A- and P-site tRNA /data/wt/unrotated/trna_a-p_PDB-9TMI_EMD-56070 picked particles - single frame - processed MRC MRC 73907 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7585

data/wt/unrotated/trna_a-p_PDB-9TMI_EMD-56070/wt.unrotated.trna_a-p.star data/wt/unrotated/trna_a-p_PDB-9TMI_EMD-56070/J229/restack/ EMD-56834: E. coli 70S ribosome with P-site tRNA /data/wt/unrotated/trna_p_EMD-56834 picked particles - single frame - processed MRC MRC 97445 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7585

data/wt/unrotated/trna_p_EMD-56834/wt.unrotated.trna_p.star data/wt/unrotated/trna_p_EMD-56834/J231/restack EMD-56835: E. coli 70S ribosome without tRNA /data/wt/unrotated/trna_vacant_EMD-56835 picked particles - single frame - processed MRC MRC 166130 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7585

data/wt/unrotated/trna_vacant_EMD-56835/wt.unrotated.trna_vacant.star data/wt/unrotated/trna_vacant_EMD-56835/J230/restack PDB 9TNL / EMD-56091: E. coli 70S ribosome with A/P- and P/E-site tRNA /data/wt/rotated/trna_ap-pe_PDB-9TNL_EMD-56091 picked particles - single frame - processed MRC MRC 69415 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7585

data/wt/rotated/trna_ap-pe_PDB-9TNL_EMD-56091/wt.rotated.trna_ap-pe.star data/wt/rotated/trna_ap-pe_PDB-9TNL_EMD-56091/J232/restack EMD-56836: E. coli 70S ribosome with A- and P/E-site tRNA /data/wt/rotated/trna_a-pe_EMD-56836 picked particles - single frame - processed MRC MRC 49259 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7585

data/wt/rotated/trna_a-pe_EMD-56836/wt.rotated.trna_a-pe.star data/wt/rotated/trna_a-pe_EMD-56836/J233/restack EMD-56837: E. coli 70S ribosome with P/E-site tRNA /data/wt/rotated/trna_pe_EMD-56837 picked particles - single frame - processed MRC MRC 34872 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7585

data/wt/rotated/trna_pe_EMD-56837/wt.rotated.trna_pe.star data/wt/rotated/trna_pe_EMD-56837/J234/restack Unaligned multi-frame micrographs of E. coli 70S ribosome, trapped conformational ground state of SSU-h44 apical loop /data/h44-top_gs/movies micrographs - multiframe TIFF TIFF 18448 50 UNSIGNED BYTE 5760 0.505 4092 0.505

Movies were collected using a Titan Krios G3i microscope (Thermo Fisher Scientific) operating at 300 kV equipped with a K3 BioQuantum detector (Gatan) at the Karolinska Institutet 3D-EM facility in Solna, Sweden. The data was acquired using EPU software (Thermo Fisher Scientific) with a pixel size of 0.505 Å/px (nominal magnification 165000x), electron dose of ~50 e−/Å2, 50 frames per movie, and a defocus range of −0.3 to −2.0 µm. The movies are gain-corrected.

data/h44-top_gs/movies/*.tiff PDB 9TQA / EMD-56128: E. coli 70S ribosome, trapped conformational ground state of SSU-h44 apical loop, with A- and P-site tRNA /data/h44-top_gs/unrotated/trna_a-p_PDB-9TQA_EMD-56128 picked particles - single frame - processed MRC MRC 81491 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7585

data/h44-top_gs/unrotated/trna_a-p_PDB-9TQA_EMD-56128/h44-top_gs.unrotated.trna_a-p.star data/h44-top_gs/unrotated/trna_a-p_PDB-9TQA_EMD-56128/J110/restack EMD-56839: E. coli 70S ribosome, trapped conformational ground state of SSU-h44 apical loop, with P-site tRNA /data/h44-top_gs/unrotated/trna_p_EMD-56839 picked particles - single frame - processed MRC MRC 86813 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7585

data/h44-top_gs/unrotated/trna_p_EMD-56839/h44-top_gs.unrotated.trna_p.star data/h44-top_gs/unrotated/trna_p_EMD-56839/J112/restack EMD-56840: E. coli 70S ribosome, trapped conformational ground state of SSU-h44 apical loop, without tRNA /data/h44-top_gs/unrotated/trna_vacant_EMD-56840 picked particles - single frame - processed MRC MRC 72339 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7585

data/h44-top_gs/unrotated/trna_vacant_EMD-56840/h44-top_gs.unrotated.trna_vacant.star data/h44-top_gs/unrotated/trna_vacant_EMD-56840/J111/restack PDB 28UH / EMD-56827: E. coli 70S ribosome, trapped conformational ground state of SSU-h44 apical loop, with A/P- and P/E-site tRNA /data/h44-top_gs/rotated/trna_ap-pe_PDB-28UH_EMD-56827 picked particles - single frame - processed MRC MRC 75660 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7585

data/h44-top_gs/rotated/trna_ap-pe_PDB-28UH_EMD-56827/h44-top_gs.rotated.trna_ap-pe.star data/h44-top_gs/rotated/trna_ap-pe_PDB-28UH_EMD-56827/J113/restack EMD-56841: E. coli 70S ribosome, trapped conformational ground state of SSU-h44 apical loop, with A- and P/E-site tRNA /data/h44-top_gs/rotated/trna_a-pe_EMD-56841 picked particles - single frame - processed MRC MRC 53199 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7585

data/h44-top_gs/rotated/trna_a-pe_EMD-56841/h44-top_gs.rotated.trna_a-pe.star data/h44-top_gs/rotated/trna_a-pe_EMD-56841/J114/restack EMD-56842: E. coli 70S ribosome, trapped conformational ground state of SSU-h44 apical loop, with P/E-site tRNA /data/h44-top_gs/rotated/trna_pe_EMD-56842 picked particles - single frame - processed MRC MRC 38095 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7585

data/h44-top_gs/rotated/trna_pe_EMD-56842/h44-top_gs.rotated.trna_pe.star data/h44-top_gs/rotated/trna_pe_EMD-56842/J115/restack Unaligned multi-frame micrographs of E. coli 70S ribosome, trapped conformational excited state of SSU-h44 apical loop /data/h44-top_es/movies micrographs - multiframe TIFF TIFF 19249 50 UNSIGNED BYTE 5760 0.505 4092 0.505

Movies were collected using a Titan Krios G3i microscope (Thermo Fisher Scientific) operating at 300 kV equipped with a K3 BioQuantum detector (Gatan) at the Karolinska Institutet 3D-EM facility in Solna, Sweden. The data was acquired using EPU software (Thermo Fisher Scientific) with a pixel size of 0.505 Å/px (nominal magnification 165000x), electron dose of ~50 e−/Å2, 50 frames per movie, and a defocus range of −0.3 to −2.0 µm. The movies are gain-corrected.

data/h44-top_es/movies/*.tiff PDB 28UI / EMD-56828: E. coli 70S ribosome, trapped conformational excited state of SSU-h44 apical loop, with A- and P-site tRNA /data/h44-top_es/unrotated/trna_a-p_PDB-28UI_EMD-56828 picked particles - single frame - processed MRC MRC 80169 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7585

data/h44-top_es/unrotated/trna_a-p_PDB-28UI_EMD-56828/h44-top_es.unrotated.trna_a-p.star data/h44-top_es/unrotated/trna_a-p_PDB-28UI_EMD-56828/J106/restack EMD-56843: E. coli 70S ribosome, trapped conformational excited state of SSU-h44 apical loop, with P-site tRNA /data/h44-top_es/unrotated/trna_p_EMD-56843 picked particles - single frame - processed MRC MRC 85044 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7585

data/h44-top_es/unrotated/trna_p_EMD-56843/h44-top_es.unrotated.trna_p.star data/h44-top_es/unrotated/trna_p_EMD-56843/J107/restack EMD-56844: E. coli 70S ribosome, trapped conformational excited state of SSU-h44 apical loop, without tRNA /data/h44-top_es/unrotated/trna_vacant_EMD-56844 picked particles - single frame - processed MRC MRC 84558 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7585

data/h44-top_es/unrotated/trna_vacant_EMD-56844/h44-top_es.unrotated.trna_vacant.star data/h44-top_es/unrotated/trna_vacant_EMD-56844/J108/restack PDB 28UJ / EMD-56829: E. coli 70S ribosome, trapped conformational excited state of SSU-h44 apical loop, with A/P- and P/E-site tRNA /data/h44-top_es/rotated/trna_ap-pe_PDB-28UJ_EMD-56829 picked particles - single frame - processed MRC MRC 62030 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7585

data/h44-top_es/rotated/trna_ap-pe_PDB-28UJ_EMD-56829/h44-top_es.rotated.trna_ap-pe.star data/h44-top_es/rotated/trna_ap-pe_PDB-28UJ_EMD-56829/J109/restack EMD-56845: E. coli 70S ribosome, trapped conformational excited state of SSU-h44 apical loop, with A- and P/E-site tRNA /data/h44-top_es/rotated/trna_a-pe_EMD-56845 picked particles - single frame - processed MRC MRC 39381 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7585

data/h44-top_es/rotated/trna_a-pe_EMD-56845/h44-top_es.rotated.trna_a-pe.star data/h44-top_es/rotated/trna_a-pe_EMD-56845/J110/restack EMD-56846: E. coli 70S ribosome, trapped conformational excited state of SSU-h44 apical loop, with P/E-site tRNA /data/h44-top_es/rotated/trna_pe_EMD-56846 picked particles - single frame - processed MRC MRC 36302 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7585

data/h44-top_es/rotated/trna_pe_EMD-56846/h44-top_es.rotated.trna_pe.star data/h44-top_es/rotated/trna_pe_EMD-56846/J111/restack Unaligned multi-frame micrographs of E. coli 70S ribosome, conformational ground state mutation in SSU-h41 /data/h41_gs/movies micrographs - multiframe TIFF TIFF 5412 50 UNSIGNED BYTE 5760 0.505 4092 0.505

Movies were collected using a Titan Krios G3i microscope (Thermo Fisher Scientific) operating at 300 kV equipped with a K3 BioQuantum detector (Gatan) at the Karolinska Institutet 3D-EM facility in Solna, Sweden. The data was acquired using EPU software (Thermo Fisher Scientific) with a pixel size of 0.505 Å/px (nominal magnification 165000x), electron dose of ~50 e−/Å2, 50 frames per movie, and a defocus range of −0.3 to −2.0 µm. The movies are gain-corrected.

data/h41_gs/movies/*.tiff PDB 28UK / EMD-56830: E. coli 70S ribosome, conformational ground state mutation in SSU-h41, with A- and P-site tRNA /data/h41_gs/unrotated/trna_a-p_PDB-28UK_EMD-56830 picked particles - single frame - processed MRC MRC 23232 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7575

data/h41_gs/unrotated/trna_a-p_PDB-28UK_EMD-56830/h41_gs.unrotated.trna_a-p.star data/h41_gs/unrotated/trna_a-p_PDB-28UK_EMD-56830/J109/restack EMD-56847: E. coli 70S ribosome, conformational ground state mutation in SSU-h41, with P-site tRNA /data/h41_gs/unrotated/trna_p_EMD-56847 picked particles - single frame - processed MRC MRC 36584 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7575

data/h41_gs/unrotated/trna_p_EMD-56847/h41_gs.unrotated.trna_p.star data/h41_gs/unrotated/trna_p_EMD-56847/J111/restack EMD-56848: E. coli 70S ribosome, conformational ground state mutation in SSU-h41, without tRNA /data/h41_gs/unrotated/trna_vacant_EMD-56848 picked particles - single frame - processed MRC MRC 31773 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7575

data/h41_gs/unrotated/trna_vacant_EMD-56848/h41_gs.unrotated.trna_vacant.star data/h41_gs/unrotated/trna_vacant_EMD-56848/J110/restack PDB 28UL / EMD-56831: E. coli 70S ribosome, conformational ground state mutation in SSU-h41, with A/P- and P/E-site tRNA /data/h41_gs/rotated/trna_ap-pe_PDB-28UL_EMD-56831 picked particles - single frame - processed MRC MRC 13469 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7575

data/h41_gs/rotated/trna_ap-pe_PDB-28UL_EMD-56831/h41_gs.rotated.trna_ap-pe.star data/h41_gs/rotated/trna_ap-pe_PDB-28UL_EMD-56831/J112/restack EMD-56849: E. coli 70S ribosome, conformational ground state mutation in SSU-h41, with A- and P/E-site tRNA /data/h41_gs/rotated/trna_a-pe_EMD-56849 picked particles - single frame - processed MRC MRC 3152 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7575

data/h41_gs/rotated/trna_a-pe_EMD-56849/h41_gs.rotated.trna_a-pe.star data/h41_gs/rotated/trna_a-pe_EMD-56849/J114/restack EMD-56850: E. coli 70S ribosome, conformational ground state mutation in SSU-h41, with P/E-site tRNA /data/h41_gs/rotated/trna_pe_EMD-56850 picked particles - single frame - processed MRC MRC 10172 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7575

data/h41_gs/rotated/trna_pe_EMD-56850/h41_gs.rotated.trna_pe.star data/h41_gs/rotated/trna_pe_EMD-56850/J113/restack Unaligned multi-frame micrographs of E. coli 70S ribosome, conformational excited state mutation in SSU-h41 /data/h41_es/movies micrographs - multiframe TIFF TIFF 6193 50 UNSIGNED BYTE 5760 0.505 4092 0.505

Movies were collected using a Titan Krios G3i microscope (Thermo Fisher Scientific) operating at 300 kV equipped with a K3 BioQuantum detector (Gatan) at the Karolinska Institutet 3D-EM facility in Solna, Sweden. The data was acquired using EPU software (Thermo Fisher Scientific) with a pixel size of 0.505 Å/px (nominal magnification 165000x), electron dose of ~50 e−/Å2, 50 frames per movie, and a defocus range of −0.3 to −2.0 µm. The movies are gain-corrected.

data/h41_es/movies/*.tiff PDB 28UM / EMD-56832: E. coli 70S ribosome, conformational excited state mutation in SSU-h41, with A- and P-site tRNA /data/h41_es/unrotated/trna_a-p_PDB-28UM_EMD-56832 picked particles - single frame - processed MRC MRC 34748 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7575

data/h41_es/unrotated/trna_a-p_PDB-28UM_EMD-56832/h41_es.unrotated.trna_a-p.star data/h41_es/unrotated/trna_a-p_PDB-28UM_EMD-56832/J261/restack EMD-56851: E. coli 70S ribosome, conformational excited state mutation in SSU-h41, with P-site tRNA /data/h41_es/unrotated/trna_p_EMD-56851 picked particles - single frame - processed MRC MRC 34669 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7575

data/h41_es/unrotated/trna_p_EMD-56851/h41_es.unrotated.trna_p.star data/h41_es/unrotated/trna_p_EMD-56851/J262/restack EMD-56852: E. coli 70S ribosome, conformational excited state mutation in SSU-h41, without tRNA /data/h41_es/unrotated/trna_vacant_EMD-56852 picked particles - single frame - processed MRC MRC 58515 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7575

data/h41_es/unrotated/trna_vacant_EMD-56852/h41_es.unrotated.trna_vacant.star data/h41_es/unrotated/trna_vacant_EMD-56852/J263/restack PDB 28UN / EMD-56833: E. coli 70S ribosome, conformational excited state mutation in SSU-h41, with A/P- and P/E-site tRNA /data/h41_es/rotated/trna_ap-pe_PDB-28UN_EMD-56833 picked particles - single frame - processed MRC MRC 14786 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7575

data/h41_es/rotated/trna_ap-pe_PDB-28UN_EMD-56833/h41_es.rotated.trna_ap-pe.star data/h41_es/rotated/trna_ap-pe_PDB-28UN_EMD-56833/J264/restack EMD-56853: E. coli 70S ribosome, conformational excited state mutation in SSU-h41, with A- and P/E-site tRNA /data/h41_es/rotated/trna_a-pe_EMD-56853 picked particles - single frame - processed MRC MRC 4860 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7575

data/h41_es/rotated/trna_a-pe_EMD-56853/h41_es.rotated.trna_a-pe.star data/h41_es/rotated/trna_a-pe_EMD-56853/J266/restack EMD-56854: E. coli 70S ribosome, conformational excited state mutation in SSU-h41, with P/E-site tRNA /data/h41_es/rotated/trna_pe_EMD-56854 picked particles - single frame - processed MRC MRC 9271 1 16 BIT FLOAT 576 0.7575 576 0.7575

Particle stack after 3D classification, reference-based motion correction, 3D variability analysis. With local + global CTF refinement and negative EWS correction. Pixel size after calibration against PDB 4YBB is 0.7575

data/h41_es/rotated/trna_pe_EMD-56854/h41_es.rotated.trna_pe.star data/h41_es/rotated/trna_pe_EMD-56854/J265/restack