REL
2026-03-18
2026-05-29
2026-05-31
Membrane insertion of mitochondrial-encoded proteins regulates ribosome decoding speed
0000-0002-4609-4052
Niels
Fischer
Depatrment of Physical Biochemistry, MPI-NAT
Am Fassberg 11
Goettingen
Lower Saxony
Germany
37077
0000-0002-4609-4052
Niels
Fischer
Depatrment of Physical Biochemistry, MPI-NAT
Am Fassberg 11
Goettingen
Germany
37077
0000-0001-5661-5272
Peter
Rehling
Department of Cellular Biochemistry, University Medical Center Goettingen
Humboldtallee 23
Goettingen
Lower Saxony
Germany
37073
Schöndorf T
Petrychenko V
Kotan I
Dahal D
Napieraj N
Cruz-Zaragoza LD
Wang C
Urbach O
Gall T
Dennerlein S
Kramer G
Fischer N
Rehling P
European Research Council (ERC)
ERCAdG No. 101054637
Germany
German Research Foundation (DFG)
SFB1565
Germany
Max Planck Society
PR, NF
Germany
German Research Foundation (DFG)
Germany’s Excellence Strategy - EXC 1199 2067/1- 390729940
Germany
3.5
10.6019/EMPIAR-13386
EMDB
molecule
EMD-55836
EMD-55837
EMD-54637
EMD-54638
EMD-55838
EMD-54639
EMD-54640
Schöndorf T
Petrychenko V
Kotan I
Dahal D
Napieraj N
Cruz-Zaragoza LD
Wang C
Urbach O
Gall T
Dennerlein S
Kramer G
Fischer N
Rehling P
Membrane insertion of mitochondrial-encoded proteins regulates ribosome decoding speed
Nature structural & molecular biology
Nat Struct Mol Biol
5
33
853
867
2026
English
10.1038/s41594-026-01803-w
42098403
Motion-corrected, dose-weighted micrographs
/data/data/Micrographs
micrographs - single frame
MRC
MRC
51805
1
32 BIT FLOAT
4096
1.16
4096
1.16
Note: Micrographs were obtained from movie-stacks of 30-40 frames à ~1 e/A^2 by global motion-correction and dose-weighting using MotionCor2 in Relion.
- Micrographs .star-file: "micrographs.star"
data/data/Micrographs/FoilHole_*_Fractions.mrc
Shiny ribosome particles, obtained by Bayesian polishing in Relion
/data/data/Particles
picked particles - single frame - processed
MRCS
MRCS
533455
1
32 BIT FLOAT
400
1.16
400
1.16
Star-files of shiny particles:
- P (EMD-55836): "particles_P.star"
- AP (EMD-55837): "particles_AP.star"
- AP* (EMD-54637): "particles_APstar.star"
- H1 (EMD-54638): "particles_H1.star"
- H2 (EMD-55838): "particles_H2.star"
- open COX1-mtRNC-OXA1L/MITRAC (EMD-54639): "particles_COX1-mtRNC-OXA1LMITRAC_open.star"
- closed COX1-mtRNC-OXA1L/MITRAC (EMD-54640): "particles_COX1-mtRNC-OXA1LMITRAC_closed.star"
Masks used for the RELION focused classifications are located in data/data/Masks
data/data/Particles/FoilHole_*_Fractions_shiny.mrcs
data/data/Particles