REL 2026-04-10 2026-04-28 2026-04-28 0000-0002-3617-7145 Naotaka Tsutsumi Institute of Science Tokyo - Cellular and Structural Physiology Laboratory (CeSPL) Bldg. 8 South B1, 1-5-45, Yushima Bunkyo-ku Tokyo Japan 113-8510 0000-0002-3617-7145 Naotaka Tsutsumi Institute of Science Tokyo - Cellular and Structural Physiology Laboratory (CeSPL) Bldg. 8 South B1, 1-5-45, Yushima, Bunkyo-ku Tokyo Japan 113-8510 Tsutsumi N Nishikawa K Fujiyoshi Y 4.5 10.6019/EMPIAR-13473 EMDB molecule EMD-65918 Drzazga AK Suzuki S Wouters C Faas F Nishikawa K Kamegawa A Fujiyoshi Y Rosenkilde MM Tsutsumi N Sathe R Communications biology Commun Biol 1 9 2026 English 10.1038/s42003-026-09660-5 41680497

This entry contains two datasets of unaligned, dark-subtracted TIFF movies (with gain references) used for the reconstruction of the UL33-Gs-Nb35 complex (EMD-65918), acquired using SerialEM with a 5×5 image shift on a 300 kV JEOL JEM-Z320FHC microscope (20 eV omega filter, Gatan K3 in CDS mode) at 0.79 Å/pixel, a defocus range of −0.6 to −1.2 μm, and a total exposure of 50 e⁻/Ų over 50 frames (2.6 s).

Cryo-EM dataset of the human Gs complexed with viral GPCR UL33 /data micrographs - multiframe TIFF TIFF 21825 50 UNSIGNED BYTE 5760 0.79 4092 0.79

This dataset comprises a total of 21,825 cryo-EM movies (.tif) and their corresponding gain reference files (.mrc) stored in the "data" folder. The data were collected using a JEOL JEM-Z320FHC operated at 300 kV, equipped with an in-column Omega energy filter and a K3 camera. Images were acquired with a pixel size of 0.79 Å/pixel, a nominal defocus range of -0.6 to -1.2 μm, and a 5 × 5 image shift pattern. The dataset is divided into two subsets based on the data collection date. The first subset in the "data/20240801" folder contains 6,925 movies, and the second in the "data/20240918" folder contains 14,900 movies. For the movie filenames, the three characters preceding the .tif extension (e.g., 001.tif) correspond to the specific beam shift position.

data/2024*/*tif