<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-13614" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2026-05-29</depositionDate>
            <releaseDate>2026-06-30</releaseDate>
            <updateDate>2026-06-30</updateDate>
        </keyDates>
        <title>CryoEM raw movies of human nucleosome core particle in nuclear extract</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-6564-4056</authorORCID>
            <firstName>De-Sheng</firstName>
            <lastName>Ker</lastName>
            <organization type="academic">Department of Biochemistry, University of Cambridge</organization>
            <street>Tennis Court Road</street>
            <townOrCity>Cambridge</townOrCity>
            <stateOrProvince>Cambridgeshire</stateOrProvince>
            <country>United Kingdom</country>
            <postOrZipCode>CB2 1GA</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-9300-497X</authorORCID>
            <firstName>Luca</firstName>
            <lastName>Pellegrini</lastName>
            <organization type="academic">Department of Biochemistry, University of Cambridge</organization>
            <street>Tennis Court Road</street>
            <townOrCity>Cambridge</townOrCity>
            <stateOrProvince>Cambridgeshire</stateOrProvince>
            <country>United Kingdom</country>
            <postOrZipCode>CB2 1GA</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-6564-4056">Ker DS</author>
            <author authorORCID="0000-0002-9300-497X">Pellegrini L</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>Wellcome Trust</fundingBody>
                <code>221892/Z/20/Z</code>
                <country>United Kingdom</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">1.1</datasetSize>
        <entryDOI>10.6019/EMPIAR-13614</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-58008</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="false" preprint="true">
                    <author authorORCID="0000-0002-6564-4056" order="1">Ker DS</author>
                    <author authorORCID="0009-0005-7683-7725" order="2">Aboalnaga H</author>
                    <author authorORCID="0000-0002-9300-497X" order="3">Pellegrini L</author>
                    <title>High resolution cryoEM of nucleosomes in nuclear extracts of mammalian cells</title>
                    <journal></journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country></country>
                    <details>Raw micrograph movies imaged using Falcon 4i detector in TIFF format.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame micrograph of nuclear extract from HEK293T</name>
        <directory>/data/Images-Disc1</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>6305</numImagesOrTiltSeries>
        <framesPerImage>50</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>50</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>4096</imageWidth>
            <pixelWidth>0.929</pixelWidth>
            <imageHeight>4096</imageHeight>
            <pixelHeight>0.929</pixelHeight>
        </dimensions>
        <details>The dataset contain the 6305 raw micrograph movies in TIFF format and gain reference files. 
The total exposure dose is 47.36 (e/A^2).</details>
        <segmentationList/>
        <micrographsFilePattern>data/Images-Disc1/GridSquare_*/Data/FoilHole_*Fractions.tiff</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
