REL 2024-03-01 2026-06-25 2026-06-25 Cryo-EM single particle micrographs of hypomethylated 80S ribosomes 0000-0003-2046-9861 Hong Li Department of Structural Biology, Van Andel Institute Grand Rapids Michigan United States 49503 0000-0003-2046-9861 Hong Li Department of Structural Biology, Van Andel Institute Grand Rapids Michigan United States 49503 Zhao Y Li H 24.2 10.6019/EMPIAR-13642 EMDB molecule EMD-40990 EMD-40991 EMD-40992 EMD-40993 EMD-71745 EMD-40997 EMD-40998 EMD-40999 EMD-41000 EMD-41001 EMD-41002 Zhao Y Rai J Cohen LN Shu Y Xu C Goswami H Chen X Jin H Marchand V Motorin Y Ghalei H Li H 2'-O-Methylation maintains ribosome structural and translation integrity Molecular cell Mol Cell 8 86 1495 1510.e7 2026 English 10.1016/j.molcel.2026.03.008 41928510 unaligned multi-frame micrographs of yeast hypomethylated 80S ribosome treated with hygromycin B /data/HygromycinB-treated micrographs - multiframe EER EER 9063 1 BIT 4096 0.365 4096 0.365
This dataset was collected on Falcon 4 camera in EER mode using Leginon, with a super-resolution pixel size of 0.365 and a total dose of 55.20 e–/Å2. The authors used EER fractionation of 32 and dose per frame of 0.82 with a binning factor 2 for motion correction.
 unaligned multi-frame micrographs of yeast nutrient limiting hypomethylated 80S ribosome /data/Nutrient-limiting micrographs - multiframe TIFF TIFF 10148 60 BIT 11520 0.53 8184 0.53
This dataset was collected on K3 camera at 81k magnification using EPU, with a super-resolution pixel size of 0.53 and a total dose of 60 e–/Å2 spreading over 60 frames. Gain file is also provided. Gain orientation in Relion: no rotation, Flip upside-down; in cryosparc: no rotation, no flip.
 unaligned multi-frame micrographs of yeast hypomethylated polysomes at 81K magnification /data/Polysome-81K micrographs - multiframe TIFF TIFF 7659 60 BIT 11520 0.53 8184 0.53
This dataset was collected on K3 camera at 81k magnification using EPU, with a super-resolution pixel size of 0.53 and a total dose of 60 e–/Å2 spreading over 60 frames. Gain file is also provided. Gain orientation in Relion: no rotation, Flip upside-down; in cryosparc: no rotation, no flip.
 unaligned multi-frame micrographs of yeast hypomethylated polysomes at 105K magnification /data/Polysome-105K micrographs - multiframe TIFF TIFF 5585 60 BIT 11520 0.415 8184 0.415
This dataset was collected on K3 camera at 81k magnification using EPU, with a super-resolution pixel size of 0.415 and a total dose of 60 e–/Å2 spreading over 60 frames. Gain file is also provided. Gain orientation in Relion: no rotation, Flip upside-down; in cryosparc: no rotation, no flip.
 unaligned multi-frame micrographs of yeast hypomethylated 80S ribosome bound with TSV-IRES, eEF2, sodarin /data/TSV-bound micrographs - multiframe TIFF TIFF 7369 60 BIT 11520 0.53 8184 0.53
This dataset was collected on K3 camera at 81k magnification using serialEM, with a super-resolution pixel size of 0.53 and a total dose of 60 e–/Å2 spreading over 60 frames. Gain file is also provided.