REL
2026-06-11
2026-06-28
2026-06-28
Cryo-EM Structures of Methylation-dependent Cas9
0000-0003-2046-9861
Hong
Li
Department of Structural Biology, Van Andel Institute
Grand Rapids
Michigan
United States
49503
0000-0003-2046-9861
Hong
Li
Department of Structural Biology, Van Andel Institute
Grand Rapids
Michigan
United States
49503
Shu Y
Zhao Y
Li H
National Institutes of Health (NIH)
S10RR025080
United States
National Institutes of Health (NIH)
U24GM116788
United States
National Institutes of Health (NIH)
S10RR024564
United States
Department of Energy (DOE, United States)
KP160711
United States
11.1
10.6019/EMPIAR-13658
EMDB
molecule
EMD-44859
EMD-43769
EMD-43771
EMD-43772
EMD-43770
Roth MO
Shu Y
Zhao Y
Trasanidou D
Hoffman RD
Südfeld C
Bouzetos E
Trasanidis N
Zawrotny M
Gelasco MK
Medina ML
Das A
Rai J
Goswami HN
Wang B
van der Oost J
Li H
Molecular basis for methylation-sensitive editing by Cas9
Nature
Nature
8116
653
1229
1239
2026
English
10.1038/s41586-026-10384-z
41986708
unaligned multi-frame micrographs of ThermoCas9 incubated with methylated DNA target with NNNNCCA PAM
/data/CC-PAM-methylatedDNA
micrographs - multiframe
MRC
MRC
6603
50
BIT
11520
0.415
8184
0.415
This dataset was collected on K3 camera at 105k magnification using EPU, with a super-resolution pixel size of 0.415 and a total dose of 60 spreading over 60 frames. Gain file is also provided. Gain orientation in Relion: no rotation, Flip upside-down; in cryosparc: no rotation, no flip.
unaligned multi-frame micrographs of ThermoCas9 incubated with non-methylated DNA target with NNNNCCA PAM
/data/CC-PAM-nonmethylatedDNA
micrographs - multiframe
MRC
MRC
6080
50
BIT
11520
0.415
8184
0.415
This dataset was collected on K3 camera at 105k magnification using EPU, with a super-resolution pixel size of 0.415 and a total dose of 60 spreading over 61 frames. Gain file is also provided. Gain orientation in Relion: no rotation, Flip upside-down; in cryosparc: no rotation, no flip.
unaligned multi-frame micrographs of ThermoCas9 incubated with non-methylated DNA target with NNNNCGA PAM
/data/CG-PAM-nonmethylatedDNA
micrographs - multiframe
MRC
MRC
12596
50
BIT
11520
0.415
8184
0.415