<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-13747" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2026-04-28</depositionDate>
            <releaseDate>2026-07-24</releaseDate>
            <updateDate>2026-07-24</updateDate>
        </keyDates>
        <title>Cryo-EM structure of ISCro4-DBL-TBL-tDNA-dDNA synaptic complex</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-7601-210X</authorORCID>
            <firstName>Martin</firstName>
            <lastName>Jinek</lastName>
            <organization type="academic">Department of Biochemistry, University of Zurich</organization>
            <street>Winterthurerstrasse 190</street>
            <townOrCity>Zurich</townOrCity>
            <stateOrProvince>ZH</stateOrProvince>
            <country>Switzerland</country>
            <postOrZipCode>8057</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-7601-210X</authorORCID>
            <firstName>Martin</firstName>
            <lastName>Jinek</lastName>
            <organization type="academic">Department of Biochemistry, University of Zurich</organization>
            <street>Winterthurerstrasse 190</street>
            <townOrCity>Zurich</townOrCity>
            <stateOrProvince>ZH</stateOrProvince>
            <country>Switzerland</country>
            <postOrZipCode>8057</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-1968-9827">Pelea O.</author>
            <author authorORCID="0009-0008-7602-1517">Fernandez Carrera J.</author>
            <author authorORCID="0009-0004-6629-036X">Gerecke S. E.</author>
            <author authorORCID="0000-0002-3432-4375">Chanez C.</author>
            <author authorORCID="0000-0002-7601-210X">Jinek M.</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>European Research Council (ERC)</fundingBody>
                <code>820152</code>
                <country>European Union</country>
            </grantReference>
            <grantReference>
                <fundingBody>Swiss National Science Foundation</fundingBody>
                <code>320030-228089</code>
                <country>Switzerland</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">1.2</datasetSize>
        <entryDOI>10.6019/EMPIAR-13747</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-55572</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0002-1968-9827" order="1">Pelea O.</author>
                    <author authorORCID="0000-0003-0851-8333" order="2">Talas A.</author>
                    <author authorORCID="0009-0008-7602-1517" order="3">Fernandez Carrera J.</author>
                    <author authorORCID="0000-0002-4051-8344" order="4">Mathis N.</author>
                    <author authorORCID="0000-0003-0158-1225" order="5">van der Venn L.</author>
                    <author authorORCID="0000-0002-2707-6035" order="6">Yeh C. D.</author>
                    <author authorORCID="0000-0002-7783-5108" order="7">Kulcsár P. I.</author>
                    <author authorORCID="0000-0001-7393-4398" order="8">Marquart K. F.</author>
                    <author authorORCID="0000-0002-0914-8541" order="9">Weber Y.</author>
                    <author authorORCID="0009-0004-6629-036X" order="10">Gerecke S. E.</author>
                    <author authorORCID="0009-0004-3417-003X" order="11">Harvey-Seutcheu I. F.</author>
                    <author authorORCID="0009-0002-8033-4926" order="12">Mailänder D.</author>
                    <author authorORCID="0000-0002-2369-5824" order="13">Pfleiderer M. M.</author>
                    <author authorORCID="0000-0002-3432-4375" order="14">Chanez C.</author>
                    <author authorORCID="0000-0002-7798-5309" order="15">Corn J.</author>
                    <author authorORCID="0000-0003-0767-2953" order="16">Schwank G.</author>
                    <author authorORCID="0000-0002-7601-210X" order="17">Jinek M.</author>
                    <title>Programmable genome editing in human cells using RNA-guided bridge recombinases</title>
                    <journal>Science</journal>
                    <journalAbbreviation>Science</journalAbbreviation>
                    <country>United States</country>
                    <issue>6790</issue>
                    <volume>391</volume>
                    <firstPage>1122</firstPage>
                    <lastPage>1122</lastPage>
                    <year>2026</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1126/science.adz188</externalReferences>
                    <externalReferences type="pubmed">41642947</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multiframe micrographs of ISCro4-DBL-TBL-tDNA-dDNA synaptic complex</name>
        <directory>/data</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>7189</numImagesOrTiltSeries>
        <framesPerImage>37</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.65</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.65</pixelHeight>
        </dimensions>
        <details>Total electron dose: 66.802 e–/Å2</details>
        <segmentationList/>
        <micrographsFilePattern>data</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Gain reference</name>
        <directory>/data</directory>
        <category>micrographs - single frame</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>1</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.65</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.65</pixelHeight>
        </dimensions>
        <details></details>
        <segmentationList/>
        <micrographsFilePattern>data/250722_Bin2x_K3-20130003 Gain Ref. x1.m1.kv[300].mrc</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
