<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-13791" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2026-07-15</depositionDate>
            <releaseDate>2026-07-24</releaseDate>
            <updateDate>2026-07-24</updateDate>
        </keyDates>
        <title>Single-particle cryo-EM datasets of macroH2A-nucleosome with scFv antibodies bound</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-6796-6745</authorORCID>
            <firstName>Dongyan</firstName>
            <lastName>Tan</lastName>
            <organization type="academic">Department of Pharmacological Sciences, Stony Brook University</organization>
            <street>101 Nicolls Rd, Stony Brook, NY</street>
            <townOrCity>Stony Brook</townOrCity>
            <stateOrProvince>NY</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>11794</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-6796-6745</authorORCID>
            <firstName>Dongyan</firstName>
            <lastName>Tan</lastName>
            <organization type="academic">Department of Pharmacological Sciences, Stony Brook University</organization>
            <street>101 Nicolls Rd, Stony Brook, NY</street>
            <townOrCity>Stony Brook</townOrCity>
            <stateOrProvince>NY</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>11794</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-6796-6745">Tan DT</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody></fundingBody>
                <code></code>
                <country></country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">5.6</datasetSize>
        <entryDOI>10.6019/EMPIAR-13791</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-71726</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="false" preprint="true">
                    <author authorORCID="0000-0002-6796-6745" order="1">Tan DT</author>
                    <title>MacroH2A-Mediated Gene Repression through Nucleosome Compaction and Remodeling Inhibition</title>
                    <journal></journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country>United States</country>
                    <year>2026</year>
                    <details>The movies are unaligned raw movies from two data collection sessions of the same sample. It contains total 19,260 files.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame movies of macroH2A nucleosome with scFv bound</name>
        <directory>/data</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>19260</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.4125</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.4125</pixelHeight>
        </dimensions>
        <details>Gain ref file (Gain-20230919-105kx.mrc) is in the "20230919_mH2A161-scFv" folder.</details>
        <segmentationList/>
        <micrographsFilePattern>data/20230801_mH2A161-scFv/raw/FoilHole_992278_Data_990747_990749_20230802_124304_fractions.tiff</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned multi-frame movies of macroH2A nucleosome with scFv bound</name>
        <directory>/data/20230919_mH2A161-scFv</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>19260</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.4125</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.4125</pixelHeight>
        </dimensions>
        <details></details>
        <segmentationList/>
        <micrographsFilePattern>data/20230919_mH2A161-scFv/frames/FoilHole_19763909_Data_19764505_19764507_20230919_132844_fractions.tiff</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
