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In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":30,"end":30,"feature_id":"monomer_feature_40","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":30,"original_end":30},{"begin":38,"end":38,"feature_id":"monomer_feature_41","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":38,"original_end":38},{"begin":39,"end":39,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. 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In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":64,"end":64,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":64,"end":64,"feature_id":"monomer_feature_44","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":64,"original_end":64},{"begin":65,"end":65,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. 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In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":65,"end":65,"feature_id":"monomer_feature_45","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":65,"original_end":65},{"begin":65,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. 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In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":68,"end":68,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":68,"end":68,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":72,"end":72,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. 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In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. 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In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":65,"end":65,"feature_id":"monomer_feature_45","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":65,"original_end":65},{"begin":65,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. 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In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":68,"end":68,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":68,"end":68,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":72,"end":72,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":79,"end":79,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":80,"end":80,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":80,"end":80,"feature_id":"monomer_feature_46","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":80,"original_end":80},{"begin":99,"end":99,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":99,"end":99,"feature_id":"monomer_feature_47","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":99,"original_end":99},{"begin":103,"end":103,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":105,"end":105,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":105,"end":105,"feature_id":"monomer_feature_48","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":105,"original_end":105},{"begin":128,"end":128,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":"monomer_feature_49","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. 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In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":149,"end":149,"feature_id":"monomer_feature_50","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":149,"original_end":149},{"begin":150,"end":150,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":153,"end":153,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":153,"end":153,"feature_id":"monomer_feature_51","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":153,"original_end":153},{"begin":155,"end":155,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":156,"end":156,"feature_id":"monomer_feature_52","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":156,"original_end":156},{"begin":157,"end":157,"feature_id":"monomer_feature_53","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":157,"original_end":157},{"begin":162,"end":162,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":163,"end":163,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":163,"end":163,"feature_id":"monomer_feature_54","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":163,"original_end":163},{"begin":164,"end":164,"feature_id":"monomer_feature_55","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":164,"original_end":164},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and V-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":171,"end":171,"feature_id":"monomer_feature_56","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":171,"original_end":171},{"begin":175,"end":175,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPA; leads to enhanced fluorescence at 37 degrees Celsius; when associated with A-163. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":176,"end":176,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":193,"end":193,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":193,"end":193,"feature_id":"monomer_feature_57","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":193,"original_end":193},{"begin":198,"end":198,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":198,"end":198,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":198,"end":198,"feature_id":"monomer_feature_58","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":198,"original_end":198},{"begin":202,"end":202,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In H9; increases fluorescence at 395 nm excitation; when associated with I-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":202,"original_end":202},{"begin":203,"end":203,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to yellow-emission variants. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and A-72. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68 and A-72. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and R-79. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and G-175.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":203,"end":203,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Suppresses the 475 nm excitation peak, leaving only the shorter wavelength peak at 399 nm. In H9; increases fluorescence at 395 nm excitation; when associated with I-203. 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In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":65,"end":65,"feature_id":"monomer_feature_45","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":65,"original_end":65},{"begin":65,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":68,"end":68,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":68,"end":68,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":72,"end":72,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":79,"end":79,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":80,"end":80,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":80,"end":80,"feature_id":"monomer_feature_46","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":80,"original_end":80},{"begin":99,"end":99,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":99,"end":99,"feature_id":"monomer_feature_47","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":99,"original_end":99},{"begin":103,"end":103,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":105,"end":105,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":105,"end":105,"feature_id":"monomer_feature_48","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":105,"original_end":105},{"begin":128,"end":128,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":"monomer_feature_49","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":147,"end":147,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":149,"end":149,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":149,"end":149,"feature_id":"monomer_feature_50","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":149,"original_end":149},{"begin":150,"end":150,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":153,"end":153,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":153,"end":153,"feature_id":"monomer_feature_51","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":153,"original_end":153},{"begin":155,"end":155,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":156,"end":156,"feature_id":"monomer_feature_52","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":156,"original_end":156},{"begin":157,"end":157,"feature_id":"monomer_feature_53","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":157,"original_end":157},{"begin":162,"end":162,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":163,"end":163,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":163,"end":163,"feature_id":"monomer_feature_54","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":163,"original_end":163},{"begin":164,"end":164,"feature_id":"monomer_feature_55","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":164,"original_end":164},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and V-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":171,"end":171,"feature_id":"monomer_feature_56","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":171,"original_end":171},{"begin":175,"end":175,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPA; leads to enhanced fluorescence at 37 degrees Celsius; when associated with A-163. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":176,"end":176,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":193,"end":193,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":193,"end":193,"feature_id":"monomer_feature_57","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":193,"original_end":193},{"begin":198,"end":198,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":198,"end":198,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":198,"end":198,"feature_id":"monomer_feature_58","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":198,"original_end":198},{"begin":202,"end":202,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In H9; increases fluorescence at 395 nm excitation; when associated with I-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":202,"original_end":202},{"begin":203,"end":203,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to yellow-emission variants. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and A-72. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68 and A-72. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and R-79. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and G-175.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":203,"end":203,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Suppresses the 475 nm excitation peak, leaving only the shorter wavelength peak at 399 nm. In H9; increases fluorescence at 395 nm excitation; when associated with I-203. 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In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":65,"end":65,"feature_id":"monomer_feature_45","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":65,"original_end":65},{"begin":65,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. 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In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":68,"end":68,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":68,"end":68,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":72,"end":72,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":79,"end":79,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":80,"end":80,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":80,"end":80,"feature_id":"monomer_feature_46","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":80,"original_end":80},{"begin":99,"end":99,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":99,"end":99,"feature_id":"monomer_feature_47","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":99,"original_end":99},{"begin":103,"end":103,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":105,"end":105,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":105,"end":105,"feature_id":"monomer_feature_48","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":105,"original_end":105},{"begin":128,"end":128,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":"monomer_feature_49","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":147,"end":147,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":149,"end":149,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":149,"end":149,"feature_id":"monomer_feature_50","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":149,"original_end":149},{"begin":150,"end":150,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":153,"end":153,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":153,"end":153,"feature_id":"monomer_feature_51","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":153,"original_end":153},{"begin":155,"end":155,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":156,"end":156,"feature_id":"monomer_feature_52","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":156,"original_end":156},{"begin":157,"end":157,"feature_id":"monomer_feature_53","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":157,"original_end":157},{"begin":162,"end":162,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":163,"end":163,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":163,"end":163,"feature_id":"monomer_feature_54","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":163,"original_end":163},{"begin":164,"end":164,"feature_id":"monomer_feature_55","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":164,"original_end":164},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and V-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":171,"end":171,"feature_id":"monomer_feature_56","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":171,"original_end":171},{"begin":175,"end":175,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPA; leads to enhanced fluorescence at 37 degrees Celsius; when associated with A-163. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":176,"end":176,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":193,"end":193,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":193,"end":193,"feature_id":"monomer_feature_57","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":193,"original_end":193},{"begin":198,"end":198,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":198,"end":198,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":198,"end":198,"feature_id":"monomer_feature_58","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":198,"original_end":198},{"begin":202,"end":202,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In H9; increases fluorescence at 395 nm excitation; when associated with I-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":202,"original_end":202},{"begin":203,"end":203,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to yellow-emission variants. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and A-72. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68 and A-72. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and R-79. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and G-175.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":203,"end":203,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Suppresses the 475 nm excitation peak, leaving only the shorter wavelength peak at 399 nm. In H9; increases fluorescence at 395 nm excitation; when associated with I-203. 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In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":65,"end":65,"feature_id":"monomer_feature_45","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":65,"original_end":65},{"begin":65,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. 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In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":68,"end":68,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":68,"end":68,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":72,"end":72,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":79,"end":79,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":80,"end":80,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":80,"end":80,"feature_id":"monomer_feature_46","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":80,"original_end":80},{"begin":99,"end":99,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":99,"end":99,"feature_id":"monomer_feature_47","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":99,"original_end":99},{"begin":103,"end":103,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":105,"end":105,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":105,"end":105,"feature_id":"monomer_feature_48","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":105,"original_end":105},{"begin":128,"end":128,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":"monomer_feature_49","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":147,"end":147,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":149,"end":149,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":149,"end":149,"feature_id":"monomer_feature_50","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":149,"original_end":149},{"begin":150,"end":150,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":153,"end":153,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":153,"end":153,"feature_id":"monomer_feature_51","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":153,"original_end":153},{"begin":155,"end":155,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":156,"end":156,"feature_id":"monomer_feature_52","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":156,"original_end":156},{"begin":157,"end":157,"feature_id":"monomer_feature_53","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":157,"original_end":157},{"begin":162,"end":162,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":163,"end":163,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":163,"end":163,"feature_id":"monomer_feature_54","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":163,"original_end":163},{"begin":164,"end":164,"feature_id":"monomer_feature_55","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":164,"original_end":164},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and V-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":171,"end":171,"feature_id":"monomer_feature_56","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":171,"original_end":171},{"begin":175,"end":175,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPA; leads to enhanced fluorescence at 37 degrees Celsius; when associated with A-163. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":176,"end":176,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":193,"end":193,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":193,"end":193,"feature_id":"monomer_feature_57","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":193,"original_end":193},{"begin":198,"end":198,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":198,"end":198,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":198,"end":198,"feature_id":"monomer_feature_58","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":198,"original_end":198},{"begin":202,"end":202,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In H9; increases fluorescence at 395 nm excitation; when associated with I-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":202,"original_end":202},{"begin":203,"end":203,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to yellow-emission variants. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and A-72. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68 and A-72. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and R-79. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and G-175.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":203,"end":203,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Suppresses the 475 nm excitation peak, leaving only the shorter wavelength peak at 399 nm. In H9; increases fluorescence at 395 nm excitation; when associated with I-203. 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In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":65,"end":65,"feature_id":"monomer_feature_45","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":65,"original_end":65},{"begin":65,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. 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In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":68,"end":68,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":68,"end":68,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":72,"end":72,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":79,"end":79,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":80,"end":80,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":80,"end":80,"feature_id":"monomer_feature_46","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":80,"original_end":80},{"begin":99,"end":99,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":99,"end":99,"feature_id":"monomer_feature_47","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":99,"original_end":99},{"begin":103,"end":103,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":105,"end":105,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":105,"end":105,"feature_id":"monomer_feature_48","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":105,"original_end":105},{"begin":128,"end":128,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":"monomer_feature_49","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":147,"end":147,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":149,"end":149,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":149,"end":149,"feature_id":"monomer_feature_50","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":149,"original_end":149},{"begin":150,"end":150,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":153,"end":153,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":153,"end":153,"feature_id":"monomer_feature_51","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":153,"original_end":153},{"begin":155,"end":155,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":156,"end":156,"feature_id":"monomer_feature_52","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":156,"original_end":156},{"begin":157,"end":157,"feature_id":"monomer_feature_53","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":157,"original_end":157},{"begin":162,"end":162,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":163,"end":163,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":163,"end":163,"feature_id":"monomer_feature_54","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":163,"original_end":163},{"begin":164,"end":164,"feature_id":"monomer_feature_55","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":164,"original_end":164},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and V-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":171,"end":171,"feature_id":"monomer_feature_56","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":171,"original_end":171},{"begin":175,"end":175,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPA; leads to enhanced fluorescence at 37 degrees Celsius; when associated with A-163. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":176,"end":176,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":193,"end":193,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":193,"end":193,"feature_id":"monomer_feature_57","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":193,"original_end":193},{"begin":198,"end":198,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":198,"end":198,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":198,"end":198,"feature_id":"monomer_feature_58","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":198,"original_end":198},{"begin":202,"end":202,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In H9; increases fluorescence at 395 nm excitation; when associated with I-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":202,"original_end":202},{"begin":203,"end":203,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to yellow-emission variants. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and A-72. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68 and A-72. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and R-79. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and G-175.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":203,"end":203,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Suppresses the 475 nm excitation peak, leaving only the shorter wavelength peak at 399 nm. In H9; increases fluorescence at 395 nm excitation; when associated with I-203. 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In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":65,"end":65,"feature_id":"monomer_feature_45","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":65,"original_end":65},{"begin":65,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. 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In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":68,"end":68,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":68,"end":68,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":72,"end":72,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":79,"end":79,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":80,"end":80,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":80,"end":80,"feature_id":"monomer_feature_46","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":80,"original_end":80},{"begin":99,"end":99,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":99,"end":99,"feature_id":"monomer_feature_47","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":99,"original_end":99},{"begin":103,"end":103,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":105,"end":105,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":105,"end":105,"feature_id":"monomer_feature_48","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":105,"original_end":105},{"begin":128,"end":128,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":"monomer_feature_49","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":147,"end":147,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":149,"end":149,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":149,"end":149,"feature_id":"monomer_feature_50","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":149,"original_end":149},{"begin":150,"end":150,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":153,"end":153,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":153,"end":153,"feature_id":"monomer_feature_51","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":153,"original_end":153},{"begin":155,"end":155,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":156,"end":156,"feature_id":"monomer_feature_52","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":156,"original_end":156},{"begin":157,"end":157,"feature_id":"monomer_feature_53","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":157,"original_end":157},{"begin":162,"end":162,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":163,"end":163,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":163,"end":163,"feature_id":"monomer_feature_54","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":163,"original_end":163},{"begin":164,"end":164,"feature_id":"monomer_feature_55","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":164,"original_end":164},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and V-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":171,"end":171,"feature_id":"monomer_feature_56","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":171,"original_end":171},{"begin":175,"end":175,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPA; leads to enhanced fluorescence at 37 degrees Celsius; when associated with A-163. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":176,"end":176,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":193,"end":193,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":193,"end":193,"feature_id":"monomer_feature_57","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":193,"original_end":193},{"begin":198,"end":198,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":198,"end":198,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":198,"end":198,"feature_id":"monomer_feature_58","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":198,"original_end":198},{"begin":202,"end":202,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In H9; increases fluorescence at 395 nm excitation; when associated with I-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":202,"original_end":202},{"begin":203,"end":203,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to yellow-emission variants. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and A-72. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68 and A-72. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and R-79. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and G-175.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":203,"end":203,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Suppresses the 475 nm excitation peak, leaving only the shorter wavelength peak at 399 nm. In H9; increases fluorescence at 395 nm excitation; when associated with I-203. 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In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":65,"end":65,"feature_id":"monomer_feature_45","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":65,"original_end":65},{"begin":65,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":68,"end":68,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":68,"end":68,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":72,"end":72,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":79,"end":79,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":80,"end":80,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":80,"end":80,"feature_id":"monomer_feature_46","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":80,"original_end":80},{"begin":99,"end":99,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":99,"end":99,"feature_id":"monomer_feature_47","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":99,"original_end":99},{"begin":103,"end":103,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":105,"end":105,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":105,"end":105,"feature_id":"monomer_feature_48","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":105,"original_end":105},{"begin":128,"end":128,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":145,"end":145,"feature_id":"monomer_feature_49","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":147,"end":147,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":149,"end":149,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":149,"end":149,"feature_id":"monomer_feature_50","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":149,"original_end":149},{"begin":150,"end":150,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":153,"end":153,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":153,"end":153,"feature_id":"monomer_feature_51","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":153,"original_end":153},{"begin":155,"end":155,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":156,"end":156,"feature_id":"monomer_feature_52","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":156,"original_end":156},{"begin":157,"end":157,"feature_id":"monomer_feature_53","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":157,"original_end":157},{"begin":162,"end":162,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":163,"end":163,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":163,"end":163,"feature_id":"monomer_feature_54","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":163,"original_end":163},{"begin":164,"end":164,"feature_id":"monomer_feature_55","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":164,"original_end":164},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and V-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":171,"end":171,"feature_id":"monomer_feature_56","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":171,"original_end":171},{"begin":175,"end":175,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPA; leads to enhanced fluorescence at 37 degrees Celsius; when associated with A-163. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":176,"end":176,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":193,"end":193,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":193,"end":193,"feature_id":"monomer_feature_57","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":193,"original_end":193},{"begin":198,"end":198,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":198,"end":198,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":198,"end":198,"feature_id":"monomer_feature_58","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_2","provenance_source":"PDB","original_begin":198,"original_end":198},{"begin":202,"end":202,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In H9; increases fluorescence at 395 nm excitation; when associated with I-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":202,"original_end":202},{"begin":203,"end":203,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to yellow-emission variants. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and A-72. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68 and A-72. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and R-79. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and G-175.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":203,"end":203,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Suppresses the 475 nm excitation peak, leaving only the shorter wavelength peak at 399 nm. In H9; increases fluorescence at 395 nm excitation; when associated with I-203. 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In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":65,"end":65,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":65,"end":65,"feature_id":"monomer_feature_9","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":65,"original_end":65},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. 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In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":66,"end":66,"feature_id":"monomer_feature_10","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":66,"original_end":66},{"begin":66,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":"monomer_feature_11","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":67,"original_end":67},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":73,"end":73,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":73,"end":73,"feature_id":"monomer_feature_12","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":73,"original_end":73},{"begin":77,"end":77,"feature_id":"monomer_feature_13","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":77,"original_end":77},{"begin":80,"end":80,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":81,"end":81,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":81,"end":81,"feature_id":"monomer_feature_14","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":81,"original_end":81},{"begin":91,"end":91,"feature_id":"monomer_feature_15","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":91,"original_end":91},{"begin":100,"end":100,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":100,"end":100,"feature_id":"monomer_feature_16","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":100,"original_end":100},{"begin":103,"end":103,"feature_id":"monomer_feature_17","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":103,"original_end":103},{"begin":104,"end":104,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":106,"end":106,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":106,"end":106,"feature_id":"monomer_feature_18","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":106,"original_end":106},{"begin":118,"end":118,"feature_id":"monomer_feature_19","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":118,"original_end":118},{"begin":125,"end":125,"feature_id":"monomer_feature_20","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":125,"original_end":125},{"begin":129,"end":129,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":129,"end":129,"feature_id":"monomer_feature_21","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":129,"original_end":129},{"begin":134,"end":134,"feature_id":"monomer_feature_22","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":134,"original_end":134},{"begin":143,"end":143,"feature_id":"monomer_feature_23","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":143,"original_end":143},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":"monomer_feature_24","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":146,"original_end":146},{"begin":147,"end":147,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":147,"end":147,"feature_id":"monomer_feature_25","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":147,"original_end":147},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":149,"end":149,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":149,"end":149,"feature_id":"monomer_feature_26","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":149,"original_end":149},{"begin":150,"end":150,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":150,"end":150,"feature_id":"monomer_feature_27","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":150,"original_end":150},{"begin":151,"end":151,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":154,"end":154,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":154,"end":154,"feature_id":"monomer_feature_28","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":154,"original_end":154},{"begin":156,"end":156,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":158,"end":158,"feature_id":"monomer_feature_29","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":158,"original_end":158},{"begin":163,"end":163,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":164,"end":164,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":164,"end":164,"feature_id":"monomer_feature_30","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":164,"original_end":164},{"begin":165,"end":165,"feature_id":"monomer_feature_31","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":165,"original_end":165},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":172,"end":172,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; S-198 and V-206. 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In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":31,"end":31,"feature_id":"monomer_feature_5","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":31,"original_end":31},{"begin":33,"end":33,"feature_id":"monomer_feature_6","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":33,"original_end":33},{"begin":35,"end":35,"feature_id":"monomer_feature_7","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":35,"original_end":35},{"begin":40,"end":40,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":39,"original_end":39},{"begin":40,"end":40,"feature_id":"monomer_feature_8","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":40,"original_end":40},{"begin":47,"end":47,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; H-69 and H-145. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-64; N-68; Q-162; A-163; V-167 and L-171. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":65,"end":65,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":65,"end":65,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":65,"end":65,"feature_id":"monomer_feature_9","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":65,"original_end":65},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with A-72. Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":66,"end":66,"feature_id":"monomer_feature_10","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":66,"original_end":66},{"begin":66,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":"monomer_feature_11","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":67,"original_end":67},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":73,"end":73,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":73,"end":73,"feature_id":"monomer_feature_12","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":73,"original_end":73},{"begin":77,"end":77,"feature_id":"monomer_feature_13","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":77,"original_end":77},{"begin":80,"end":80,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":81,"end":81,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":81,"end":81,"feature_id":"monomer_feature_14","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":81,"original_end":81},{"begin":91,"end":91,"feature_id":"monomer_feature_15","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":91,"original_end":91},{"begin":100,"end":100,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":100,"end":100,"feature_id":"monomer_feature_16","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":100,"original_end":100},{"begin":103,"end":103,"feature_id":"monomer_feature_17","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":103,"original_end":103},{"begin":104,"end":104,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":106,"end":106,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":106,"end":106,"feature_id":"monomer_feature_18","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":106,"original_end":106},{"begin":118,"end":118,"feature_id":"monomer_feature_19","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":118,"original_end":118},{"begin":125,"end":125,"feature_id":"monomer_feature_20","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":125,"original_end":125},{"begin":129,"end":129,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":129,"end":129,"feature_id":"monomer_feature_21","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":129,"original_end":129},{"begin":134,"end":134,"feature_id":"monomer_feature_22","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":134,"original_end":134},{"begin":143,"end":143,"feature_id":"monomer_feature_23","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":143,"original_end":143},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":"monomer_feature_24","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":146,"original_end":146},{"begin":147,"end":147,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. 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In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":31,"end":31,"feature_id":"monomer_feature_5","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":31,"original_end":31},{"begin":33,"end":33,"feature_id":"monomer_feature_6","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":33,"original_end":33},{"begin":35,"end":35,"feature_id":"monomer_feature_7","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":35,"original_end":35},{"begin":40,"end":40,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. 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In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":65,"end":65,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":65,"end":65,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":65,"end":65,"feature_id":"monomer_feature_9","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":65,"original_end":65},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. 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In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":66,"end":66,"feature_id":"monomer_feature_10","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":66,"original_end":66},{"begin":66,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. 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In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. 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In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":"monomer_feature_11","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":67,"original_end":67},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":73,"end":73,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. 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In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":73,"end":73,"feature_id":"monomer_feature_12","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":73,"original_end":73},{"begin":77,"end":77,"feature_id":"monomer_feature_13","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":77,"original_end":77},{"begin":80,"end":80,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":81,"end":81,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":81,"end":81,"feature_id":"monomer_feature_14","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":81,"original_end":81},{"begin":91,"end":91,"feature_id":"monomer_feature_15","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":91,"original_end":91},{"begin":100,"end":100,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":100,"end":100,"feature_id":"monomer_feature_16","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":100,"original_end":100},{"begin":103,"end":103,"feature_id":"monomer_feature_17","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":103,"original_end":103},{"begin":104,"end":104,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":106,"end":106,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. 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In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":"monomer_feature_24","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":146,"original_end":146},{"begin":147,"end":147,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. 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In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":154,"end":154,"feature_id":"monomer_feature_28","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":154,"original_end":154},{"begin":156,"end":156,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":158,"end":158,"feature_id":"monomer_feature_29","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":158,"original_end":158},{"begin":163,"end":163,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":164,"end":164,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. 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In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":164,"end":164,"feature_id":"monomer_feature_30","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":164,"original_end":164},{"begin":165,"end":165,"feature_id":"monomer_feature_31","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":165,"original_end":165},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. 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In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":177,"end":177,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":191,"end":191,"feature_id":"monomer_feature_34","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":191,"original_end":191},{"begin":194,"end":194,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":198,"end":198,"feature_id":"monomer_feature_35","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":198,"original_end":198},{"begin":199,"end":199,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":199,"end":199,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":203,"end":203,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In H9; increases fluorescence at 395 nm excitation; when associated with I-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":202,"original_end":202},{"begin":204,"end":204,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to yellow-emission variants. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and A-72. 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In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":65,"end":65,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":65,"end":65,"feature_id":"monomer_feature_9","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":65,"original_end":65},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. 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In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":66,"end":66,"feature_id":"monomer_feature_10","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":66,"original_end":66},{"begin":66,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":"monomer_feature_11","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":67,"original_end":67},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":73,"end":73,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":73,"end":73,"feature_id":"monomer_feature_12","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":73,"original_end":73},{"begin":77,"end":77,"feature_id":"monomer_feature_13","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":77,"original_end":77},{"begin":80,"end":80,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":81,"end":81,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":81,"end":81,"feature_id":"monomer_feature_14","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":81,"original_end":81},{"begin":91,"end":91,"feature_id":"monomer_feature_15","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":91,"original_end":91},{"begin":100,"end":100,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":100,"end":100,"feature_id":"monomer_feature_16","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":100,"original_end":100},{"begin":103,"end":103,"feature_id":"monomer_feature_17","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":103,"original_end":103},{"begin":104,"end":104,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":106,"end":106,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":106,"end":106,"feature_id":"monomer_feature_18","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":106,"original_end":106},{"begin":118,"end":118,"feature_id":"monomer_feature_19","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":118,"original_end":118},{"begin":125,"end":125,"feature_id":"monomer_feature_20","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":125,"original_end":125},{"begin":129,"end":129,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":129,"end":129,"feature_id":"monomer_feature_21","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":129,"original_end":129},{"begin":134,"end":134,"feature_id":"monomer_feature_22","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":134,"original_end":134},{"begin":143,"end":143,"feature_id":"monomer_feature_23","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":143,"original_end":143},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":"monomer_feature_24","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":146,"original_end":146},{"begin":147,"end":147,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":147,"end":147,"feature_id":"monomer_feature_25","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":147,"original_end":147},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":149,"end":149,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":149,"end":149,"feature_id":"monomer_feature_26","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":149,"original_end":149},{"begin":150,"end":150,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":150,"end":150,"feature_id":"monomer_feature_27","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":150,"original_end":150},{"begin":151,"end":151,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":154,"end":154,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":154,"end":154,"feature_id":"monomer_feature_28","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":154,"original_end":154},{"begin":156,"end":156,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":158,"end":158,"feature_id":"monomer_feature_29","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":158,"original_end":158},{"begin":163,"end":163,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":164,"end":164,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":164,"end":164,"feature_id":"monomer_feature_30","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":164,"original_end":164},{"begin":165,"end":165,"feature_id":"monomer_feature_31","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":165,"original_end":165},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":172,"end":172,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; S-198 and V-206. 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In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":31,"end":31,"feature_id":"monomer_feature_5","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":31,"original_end":31},{"begin":33,"end":33,"feature_id":"monomer_feature_6","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":33,"original_end":33},{"begin":35,"end":35,"feature_id":"monomer_feature_7","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":35,"original_end":35},{"begin":40,"end":40,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":39,"original_end":39},{"begin":40,"end":40,"feature_id":"monomer_feature_8","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":40,"original_end":40},{"begin":47,"end":47,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; H-69 and H-145. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-64; N-68; Q-162; A-163; V-167 and L-171. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":65,"end":65,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":65,"end":65,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":65,"end":65,"feature_id":"monomer_feature_9","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":65,"original_end":65},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with A-72. Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":66,"end":66,"feature_id":"monomer_feature_10","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":66,"original_end":66},{"begin":66,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":"monomer_feature_11","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":67,"original_end":67},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":73,"end":73,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":73,"end":73,"feature_id":"monomer_feature_12","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":73,"original_end":73},{"begin":77,"end":77,"feature_id":"monomer_feature_13","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":77,"original_end":77},{"begin":80,"end":80,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":81,"end":81,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":81,"end":81,"feature_id":"monomer_feature_14","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":81,"original_end":81},{"begin":91,"end":91,"feature_id":"monomer_feature_15","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":91,"original_end":91},{"begin":100,"end":100,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":100,"end":100,"feature_id":"monomer_feature_16","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":100,"original_end":100},{"begin":103,"end":103,"feature_id":"monomer_feature_17","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":103,"original_end":103},{"begin":104,"end":104,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":106,"end":106,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":106,"end":106,"feature_id":"monomer_feature_18","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":106,"original_end":106},{"begin":118,"end":118,"feature_id":"monomer_feature_19","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":118,"original_end":118},{"begin":125,"end":125,"feature_id":"monomer_feature_20","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":125,"original_end":125},{"begin":129,"end":129,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":129,"end":129,"feature_id":"monomer_feature_21","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":129,"original_end":129},{"begin":134,"end":134,"feature_id":"monomer_feature_22","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":134,"original_end":134},{"begin":143,"end":143,"feature_id":"monomer_feature_23","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":143,"original_end":143},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":"monomer_feature_24","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":146,"original_end":146},{"begin":147,"end":147,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. 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In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":31,"end":31,"feature_id":"monomer_feature_5","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":31,"original_end":31},{"begin":33,"end":33,"feature_id":"monomer_feature_6","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":33,"original_end":33},{"begin":35,"end":35,"feature_id":"monomer_feature_7","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":35,"original_end":35},{"begin":40,"end":40,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. 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In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":65,"end":65,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":65,"end":65,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":65,"end":65,"feature_id":"monomer_feature_9","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":65,"original_end":65},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. 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In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":66,"end":66,"feature_id":"monomer_feature_10","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":66,"original_end":66},{"begin":66,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. 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In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. 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In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":"monomer_feature_11","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":67,"original_end":67},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":73,"end":73,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. 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In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":73,"end":73,"feature_id":"monomer_feature_12","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":73,"original_end":73},{"begin":77,"end":77,"feature_id":"monomer_feature_13","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":77,"original_end":77},{"begin":80,"end":80,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":81,"end":81,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":81,"end":81,"feature_id":"monomer_feature_14","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":81,"original_end":81},{"begin":91,"end":91,"feature_id":"monomer_feature_15","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":91,"original_end":91},{"begin":100,"end":100,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":100,"end":100,"feature_id":"monomer_feature_16","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":100,"original_end":100},{"begin":103,"end":103,"feature_id":"monomer_feature_17","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":103,"original_end":103},{"begin":104,"end":104,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":106,"end":106,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. 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In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":"monomer_feature_24","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":146,"original_end":146},{"begin":147,"end":147,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. 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In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":154,"end":154,"feature_id":"monomer_feature_28","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":154,"original_end":154},{"begin":156,"end":156,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":158,"end":158,"feature_id":"monomer_feature_29","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":158,"original_end":158},{"begin":163,"end":163,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":164,"end":164,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. 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In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":164,"end":164,"feature_id":"monomer_feature_30","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":164,"original_end":164},{"begin":165,"end":165,"feature_id":"monomer_feature_31","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":165,"original_end":165},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. 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In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":177,"end":177,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":191,"end":191,"feature_id":"monomer_feature_34","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":191,"original_end":191},{"begin":194,"end":194,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":198,"end":198,"feature_id":"monomer_feature_35","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":198,"original_end":198},{"begin":199,"end":199,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":199,"end":199,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":203,"end":203,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In H9; increases fluorescence at 395 nm excitation; when associated with I-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":202,"original_end":202},{"begin":204,"end":204,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to yellow-emission variants. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and A-72. 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In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":65,"end":65,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":65,"end":65,"feature_id":"monomer_feature_9","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":65,"original_end":65},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. 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In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":66,"end":66,"feature_id":"monomer_feature_10","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":66,"original_end":66},{"begin":66,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":"monomer_feature_11","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":67,"original_end":67},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":73,"end":73,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":73,"end":73,"feature_id":"monomer_feature_12","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":73,"original_end":73},{"begin":77,"end":77,"feature_id":"monomer_feature_13","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":77,"original_end":77},{"begin":80,"end":80,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":81,"end":81,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":81,"end":81,"feature_id":"monomer_feature_14","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":81,"original_end":81},{"begin":91,"end":91,"feature_id":"monomer_feature_15","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":91,"original_end":91},{"begin":100,"end":100,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":100,"end":100,"feature_id":"monomer_feature_16","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":100,"original_end":100},{"begin":103,"end":103,"feature_id":"monomer_feature_17","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":103,"original_end":103},{"begin":104,"end":104,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":106,"end":106,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":106,"end":106,"feature_id":"monomer_feature_18","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":106,"original_end":106},{"begin":118,"end":118,"feature_id":"monomer_feature_19","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":118,"original_end":118},{"begin":125,"end":125,"feature_id":"monomer_feature_20","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":125,"original_end":125},{"begin":129,"end":129,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":129,"end":129,"feature_id":"monomer_feature_21","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":129,"original_end":129},{"begin":134,"end":134,"feature_id":"monomer_feature_22","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":134,"original_end":134},{"begin":143,"end":143,"feature_id":"monomer_feature_23","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":143,"original_end":143},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":"monomer_feature_24","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":146,"original_end":146},{"begin":147,"end":147,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":147,"end":147,"feature_id":"monomer_feature_25","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":147,"original_end":147},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":149,"end":149,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":149,"end":149,"feature_id":"monomer_feature_26","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":149,"original_end":149},{"begin":150,"end":150,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":150,"end":150,"feature_id":"monomer_feature_27","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":150,"original_end":150},{"begin":151,"end":151,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":154,"end":154,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":154,"end":154,"feature_id":"monomer_feature_28","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":154,"original_end":154},{"begin":156,"end":156,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":158,"end":158,"feature_id":"monomer_feature_29","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":158,"original_end":158},{"begin":163,"end":163,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":164,"end":164,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":164,"end":164,"feature_id":"monomer_feature_30","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":164,"original_end":164},{"begin":165,"end":165,"feature_id":"monomer_feature_31","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":165,"original_end":165},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":172,"end":172,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; S-198 and V-206. 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In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":31,"end":31,"feature_id":"monomer_feature_5","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":31,"original_end":31},{"begin":33,"end":33,"feature_id":"monomer_feature_6","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":33,"original_end":33},{"begin":35,"end":35,"feature_id":"monomer_feature_7","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":35,"original_end":35},{"begin":40,"end":40,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":39,"original_end":39},{"begin":40,"end":40,"feature_id":"monomer_feature_8","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":40,"original_end":40},{"begin":47,"end":47,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; H-69 and H-145. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-64; N-68; Q-162; A-163; V-167 and L-171. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":65,"end":65,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":65,"end":65,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":65,"end":65,"feature_id":"monomer_feature_9","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":65,"original_end":65},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with A-72. Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":66,"end":66,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":66,"end":66,"feature_id":"monomer_feature_10","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":66,"original_end":66},{"begin":66,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":67,"end":67,"feature_id":"monomer_feature_11","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":67,"original_end":67},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":69,"end":69,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":70,"end":70,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":73,"end":73,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":73,"end":73,"feature_id":"monomer_feature_12","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":73,"original_end":73},{"begin":77,"end":77,"feature_id":"monomer_feature_13","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":77,"original_end":77},{"begin":80,"end":80,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":81,"end":81,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":81,"end":81,"feature_id":"monomer_feature_14","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":81,"original_end":81},{"begin":91,"end":91,"feature_id":"monomer_feature_15","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":91,"original_end":91},{"begin":100,"end":100,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":100,"end":100,"feature_id":"monomer_feature_16","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":100,"original_end":100},{"begin":103,"end":103,"feature_id":"monomer_feature_17","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":103,"original_end":103},{"begin":104,"end":104,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":106,"end":106,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":106,"end":106,"feature_id":"monomer_feature_18","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":106,"original_end":106},{"begin":118,"end":118,"feature_id":"monomer_feature_19","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":118,"original_end":118},{"begin":125,"end":125,"feature_id":"monomer_feature_20","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":125,"original_end":125},{"begin":129,"end":129,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":129,"end":129,"feature_id":"monomer_feature_21","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":129,"original_end":129},{"begin":134,"end":134,"feature_id":"monomer_feature_22","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":134,"original_end":134},{"begin":143,"end":143,"feature_id":"monomer_feature_23","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":143,"original_end":143},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":146,"end":146,"feature_id":"monomer_feature_24","type":"MUTATION","name":"engineered mutation","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MDR_1","provenance_source":"PDB","original_begin":146,"original_end":146},{"begin":147,"end":147,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. 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