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In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":141,"end":141,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":39,"original_end":39},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; H-69 and H-145. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-64; N-68; Q-162; A-163; V-167 and L-171. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with A-72. Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":249,"end":249,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":250,"end":250,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":251,"end":251,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":252,"end":252,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":255,"end":255,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. 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In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":249,"end":249,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":250,"end":250,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":251,"end":251,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":252,"end":252,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":255,"end":255,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and V-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":277,"end":277,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPA; leads to enhanced fluorescence at 37 degrees Celsius; when associated with A-163. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":278,"end":278,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":295,"end":295,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":304,"end":304,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In H9; increases fluorescence at 395 nm excitation; when associated with I-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":202,"original_end":202},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to yellow-emission variants. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and A-72. 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In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":249,"end":249,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":250,"end":250,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":251,"end":251,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":252,"end":252,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":255,"end":255,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and V-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":277,"end":277,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPA; leads to enhanced fluorescence at 37 degrees Celsius; when associated with A-163. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":278,"end":278,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":295,"end":295,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":304,"end":304,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In H9; increases fluorescence at 395 nm excitation; when associated with I-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":202,"original_end":202},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to yellow-emission variants. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and A-72. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68 and A-72. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and R-79. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and G-175.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Suppresses the 475 nm excitation peak, leaving only the shorter wavelength peak at 399 nm. In H9; increases fluorescence at 395 nm excitation; when associated with I-203. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and F-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Results in significantly red-shifted excitation and emission maxima.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":308,"end":308,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and S-198. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-206.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":206,"original_end":206},{"begin":308,"end":308,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Abolishes the tendency to dimerize and leads to monomeric fluorescent proteins.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":206,"original_end":206},{"begin":324,"end":324,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Suppresses the 399 nm excitation peak, leaving only the longer wavelength peak at 475 nm.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":222,"original_end":222},{"begin":326,"end":326,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and I-150. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-206.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":224,"original_end":224}]},{"key":"other_artifact","label":"ARTIFACT","kind":"range","features":[{"begin":1,"end":3,"feature_id":"range_feature_1","type":"ARTIFACT","name":"expression tag","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MKS_1","provenance_source":"PDB","original_begin":1,"original_end":3},{"begin":100,"end":101,"feature_id":"range_feature_2","type":"ARTIFACT","name":"linker","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MKS_1","provenance_source":"PDB","original_begin":100,"original_end":101},{"begin":334,"end":340,"feature_id":"range_feature_3","type":"ARTIFACT","name":"expression 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4","value":null,"source":"UNIPROT","source_id":"Q9NPP4","provenance_source":null,"original_begin":1,"original_end":96},{"begin":103,"end":332,"feature_id":"PRO_0000192576","type":"CHAIN","name":null,"description":"Green fluorescent protein","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":1,"original_end":230}]},{"key":"mutagenesis","label":"MUTAGENESIS","kind":"point","features":[{"begin":132,"end":132,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with H-145. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with H-69 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with L-46; H-69 and H-145. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":141,"end":141,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":39,"original_end":39},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; H-69 and H-145. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-64; N-68; Q-162; A-163; V-167 and L-171. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with A-72. Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. 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In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. 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In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. 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4","value":null,"source":"UNIPROT","source_id":"Q9NPP4","provenance_source":null,"original_begin":1,"original_end":96},{"begin":103,"end":332,"feature_id":"PRO_0000192576","type":"CHAIN","name":null,"description":"Green fluorescent protein","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":1,"original_end":230}]},{"key":"mutagenesis","label":"MUTAGENESIS","kind":"point","features":[{"begin":132,"end":132,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with H-145. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with H-69 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with L-46; H-69 and H-145. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":141,"end":141,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":39,"original_end":39},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; H-69 and H-145. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-64; N-68; Q-162; A-163; V-167 and L-171. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with A-72. Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. 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In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":252,"end":252,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. 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In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":141,"end":141,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":39,"original_end":39},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; H-69 and H-145. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-64; N-68; Q-162; A-163; V-167 and L-171. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with A-72. Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":249,"end":249,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":250,"end":250,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":251,"end":251,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":252,"end":252,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":255,"end":255,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. 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In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":249,"end":249,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":250,"end":250,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":251,"end":251,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":252,"end":252,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":255,"end":255,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and V-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":277,"end":277,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPA; leads to enhanced fluorescence at 37 degrees Celsius; when associated with A-163. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":278,"end":278,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":295,"end":295,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":304,"end":304,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In H9; increases fluorescence at 395 nm excitation; when associated with I-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":202,"original_end":202},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to yellow-emission variants. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and A-72. 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Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. 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In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":249,"end":249,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":250,"end":250,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":251,"end":251,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":252,"end":252,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":255,"end":255,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and V-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":277,"end":277,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPA; leads to enhanced fluorescence at 37 degrees Celsius; when associated with A-163. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":278,"end":278,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":295,"end":295,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":304,"end":304,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In H9; increases fluorescence at 395 nm excitation; when associated with I-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":202,"original_end":202},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to yellow-emission variants. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and A-72. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68 and A-72. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and R-79. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and G-175.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Suppresses the 475 nm excitation peak, leaving only the shorter wavelength peak at 399 nm. In H9; increases fluorescence at 395 nm excitation; when associated with I-203. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and F-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Results in significantly red-shifted excitation and emission maxima.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":308,"end":308,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and S-198. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-206.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":206,"original_end":206},{"begin":308,"end":308,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Abolishes the tendency to dimerize and leads to monomeric fluorescent proteins.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":206,"original_end":206},{"begin":324,"end":324,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Suppresses the 399 nm excitation peak, leaving only the longer wavelength peak at 475 nm.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":222,"original_end":222},{"begin":326,"end":326,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and I-150. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-206.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":224,"original_end":224}]},{"key":"other_artifact","label":"ARTIFACT","kind":"range","features":[{"begin":1,"end":3,"feature_id":"range_feature_1","type":"ARTIFACT","name":"expression tag","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MKS_1","provenance_source":"PDB","original_begin":1,"original_end":3},{"begin":100,"end":101,"feature_id":"range_feature_2","type":"ARTIFACT","name":"linker","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MKS_1","provenance_source":"PDB","original_begin":100,"original_end":101},{"begin":334,"end":340,"feature_id":"range_feature_3","type":"ARTIFACT","name":"expression 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4","value":null,"source":"UNIPROT","source_id":"Q9NPP4","provenance_source":null,"original_begin":1,"original_end":96},{"begin":103,"end":332,"feature_id":"PRO_0000192576","type":"CHAIN","name":null,"description":"Green fluorescent protein","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":1,"original_end":230}]},{"key":"mutagenesis","label":"MUTAGENESIS","kind":"point","features":[{"begin":132,"end":132,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with H-145. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with H-69 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with L-46; H-69 and H-145. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":141,"end":141,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":39,"original_end":39},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; H-69 and H-145. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-64; N-68; Q-162; A-163; V-167 and L-171. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with A-72. Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. 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In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. 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In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. 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In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and F-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Results in significantly red-shifted excitation and emission maxima.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":308,"end":308,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and S-198. 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In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with H-69 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with L-46; H-69 and H-145. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":141,"end":141,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. 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In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with A-72. Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. 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In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with H-69 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with L-46; H-69 and H-145. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":141,"end":141,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":39,"original_end":39},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; H-69 and H-145. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-64; N-68; Q-162; A-163; V-167 and L-171. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with A-72. Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":249,"end":249,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":250,"end":250,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":251,"end":251,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":252,"end":252,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":255,"end":255,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. 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In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. 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In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":249,"end":249,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":250,"end":250,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":251,"end":251,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":252,"end":252,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":255,"end":255,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and V-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":277,"end":277,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPA; leads to enhanced fluorescence at 37 degrees Celsius; when associated with A-163. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":278,"end":278,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":295,"end":295,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":304,"end":304,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In H9; increases fluorescence at 395 nm excitation; when associated with I-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":202,"original_end":202},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to yellow-emission variants. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and A-72. 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Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. 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In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":249,"end":249,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":250,"end":250,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":251,"end":251,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":252,"end":252,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":255,"end":255,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and V-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":277,"end":277,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPA; leads to enhanced fluorescence at 37 degrees Celsius; when associated with A-163. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":278,"end":278,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":295,"end":295,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":304,"end":304,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In H9; increases fluorescence at 395 nm excitation; when associated with I-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":202,"original_end":202},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to yellow-emission variants. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and A-72. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68 and A-72. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and R-79. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and G-175.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Suppresses the 475 nm excitation peak, leaving only the shorter wavelength peak at 399 nm. In H9; increases fluorescence at 395 nm excitation; when associated with I-203. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and F-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Results in significantly red-shifted excitation and emission maxima.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":308,"end":308,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and S-198. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-206.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":206,"original_end":206},{"begin":308,"end":308,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Abolishes the tendency to dimerize and leads to monomeric fluorescent proteins.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":206,"original_end":206},{"begin":324,"end":324,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Suppresses the 399 nm excitation peak, leaving only the longer wavelength peak at 475 nm.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":222,"original_end":222},{"begin":326,"end":326,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and I-150. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-206.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":224,"original_end":224}]},{"key":"other_artifact","label":"ARTIFACT","kind":"range","features":[{"begin":1,"end":3,"feature_id":"range_feature_1","type":"ARTIFACT","name":"expression tag","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MKS_1","provenance_source":"PDB","original_begin":1,"original_end":3},{"begin":100,"end":101,"feature_id":"range_feature_2","type":"ARTIFACT","name":"linker","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MKS_1","provenance_source":"PDB","original_begin":100,"original_end":101},{"begin":334,"end":340,"feature_id":"range_feature_3","type":"ARTIFACT","name":"expression 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4","value":null,"source":"UNIPROT","source_id":"Q9NPP4","provenance_source":null,"original_begin":1,"original_end":96},{"begin":103,"end":332,"feature_id":"PRO_0000192576","type":"CHAIN","name":null,"description":"Green fluorescent protein","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":1,"original_end":230}]},{"key":"mutagenesis","label":"MUTAGENESIS","kind":"point","features":[{"begin":132,"end":132,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with H-145. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with H-69 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with L-46; H-69 and H-145. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":141,"end":141,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":39,"original_end":39},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; H-69 and H-145. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-64; N-68; Q-162; A-163; V-167 and L-171. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with A-72. Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. 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In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. 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In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. 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In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and V-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":277,"end":277,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPA; leads to enhanced fluorescence at 37 degrees Celsius; when associated with A-163. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":278,"end":278,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":295,"end":295,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. 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In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and F-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Results in significantly red-shifted excitation and emission maxima.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":308,"end":308,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and S-198. 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4","value":null,"source":"UNIPROT","source_id":"Q9NPP4","provenance_source":null,"original_begin":1,"original_end":96},{"begin":103,"end":332,"feature_id":"PRO_0000192576","type":"CHAIN","name":null,"description":"Green fluorescent protein","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":1,"original_end":230}]},{"key":"mutagenesis","label":"MUTAGENESIS","kind":"point","features":[{"begin":132,"end":132,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with H-145. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with H-69 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with L-46; H-69 and H-145. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":141,"end":141,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":39,"original_end":39},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; H-69 and H-145. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-64; N-68; Q-162; A-163; V-167 and L-171. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with A-72. Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. 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In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":252,"end":252,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. 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In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":141,"end":141,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":39,"original_end":39},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; H-69 and H-145. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-64; N-68; Q-162; A-163; V-167 and L-171. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with A-72. Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":249,"end":249,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":250,"end":250,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":251,"end":251,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":252,"end":252,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":255,"end":255,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. 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In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":249,"end":249,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":250,"end":250,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":251,"end":251,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":252,"end":252,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":255,"end":255,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and V-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":277,"end":277,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPA; leads to enhanced fluorescence at 37 degrees Celsius; when associated with A-163. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":278,"end":278,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":295,"end":295,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":304,"end":304,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In H9; increases fluorescence at 395 nm excitation; when associated with I-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":202,"original_end":202},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to yellow-emission variants. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and A-72. 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In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":249,"end":249,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":250,"end":250,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":251,"end":251,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":252,"end":252,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":255,"end":255,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and V-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":277,"end":277,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPA; leads to enhanced fluorescence at 37 degrees Celsius; when associated with A-163. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":278,"end":278,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":295,"end":295,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":304,"end":304,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In H9; increases fluorescence at 395 nm excitation; when associated with I-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":202,"original_end":202},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to yellow-emission variants. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and A-72. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68 and A-72. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and R-79. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and G-175.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Suppresses the 475 nm excitation peak, leaving only the shorter wavelength peak at 399 nm. In H9; increases fluorescence at 395 nm excitation; when associated with I-203. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and F-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Results in significantly red-shifted excitation and emission maxima.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":308,"end":308,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and S-198. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-206.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":206,"original_end":206},{"begin":308,"end":308,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Abolishes the tendency to dimerize and leads to monomeric fluorescent proteins.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":206,"original_end":206},{"begin":324,"end":324,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Suppresses the 399 nm excitation peak, leaving only the longer wavelength peak at 475 nm.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":222,"original_end":222},{"begin":326,"end":326,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and I-150. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-206.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":224,"original_end":224}]},{"key":"other_artifact","label":"ARTIFACT","kind":"range","features":[{"begin":1,"end":3,"feature_id":"range_feature_1","type":"ARTIFACT","name":"expression tag","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MKS_1","provenance_source":"PDB","original_begin":1,"original_end":3},{"begin":100,"end":101,"feature_id":"range_feature_2","type":"ARTIFACT","name":"linker","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MKS_1","provenance_source":"PDB","original_begin":100,"original_end":101},{"begin":334,"end":340,"feature_id":"range_feature_3","type":"ARTIFACT","name":"expression 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4","value":null,"source":"UNIPROT","source_id":"Q9NPP4","provenance_source":null,"original_begin":1,"original_end":96},{"begin":103,"end":332,"feature_id":"PRO_0000192576","type":"CHAIN","name":null,"description":"Green fluorescent protein","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":1,"original_end":230}]},{"key":"mutagenesis","label":"MUTAGENESIS","kind":"point","features":[{"begin":132,"end":132,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with H-145. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with H-69 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with L-46; H-69 and H-145. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":141,"end":141,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":39,"original_end":39},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; H-69 and H-145. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-64; N-68; Q-162; A-163; V-167 and L-171. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with A-72. Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. 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In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. 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In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. 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In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and V-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":277,"end":277,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPA; leads to enhanced fluorescence at 37 degrees Celsius; when associated with A-163. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":278,"end":278,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":295,"end":295,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. 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In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and F-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Results in significantly red-shifted excitation and emission maxima.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":308,"end":308,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and S-198. 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In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with H-69 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with L-46; H-69 and H-145. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":141,"end":141,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. 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In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with A-72. Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. 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In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with H-69 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with L-46; H-69 and H-145. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":141,"end":141,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":39,"original_end":39},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; H-69 and H-145. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-64; N-68; Q-162; A-163; V-167 and L-171. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with A-72. Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":249,"end":249,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":250,"end":250,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":251,"end":251,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":252,"end":252,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":255,"end":255,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. 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In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":249,"end":249,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":250,"end":250,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":251,"end":251,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":252,"end":252,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":255,"end":255,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and V-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":277,"end":277,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPA; leads to enhanced fluorescence at 37 degrees Celsius; when associated with A-163. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":278,"end":278,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":295,"end":295,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":304,"end":304,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In H9; increases fluorescence at 395 nm excitation; when associated with I-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":202,"original_end":202},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to yellow-emission variants. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and A-72. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68 and A-72. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and R-79. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and G-175.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Suppresses the 475 nm excitation peak, leaving only the shorter wavelength peak at 399 nm. In H9; increases fluorescence at 395 nm excitation; when associated with I-203. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and F-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Results in significantly red-shifted excitation and emission maxima.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":308,"end":308,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and S-198. 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Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. 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In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":249,"end":249,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":250,"end":250,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":251,"end":251,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":252,"end":252,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":255,"end":255,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and V-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":277,"end":277,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPA; leads to enhanced fluorescence at 37 degrees Celsius; when associated with A-163. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":278,"end":278,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":295,"end":295,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":304,"end":304,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In H9; increases fluorescence at 395 nm excitation; when associated with I-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":202,"original_end":202},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to yellow-emission variants. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and A-72. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68 and A-72. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and R-79. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and G-175.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Suppresses the 475 nm excitation peak, leaving only the shorter wavelength peak at 399 nm. In H9; increases fluorescence at 395 nm excitation; when associated with I-203. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and F-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Results in significantly red-shifted excitation and emission maxima.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":308,"end":308,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and S-198. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-206.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":206,"original_end":206},{"begin":308,"end":308,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Abolishes the tendency to dimerize and leads to monomeric fluorescent proteins.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":206,"original_end":206},{"begin":324,"end":324,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Suppresses the 399 nm excitation peak, leaving only the longer wavelength peak at 475 nm.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":222,"original_end":222},{"begin":326,"end":326,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and I-150. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-206.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":224,"original_end":224}]},{"key":"other_artifact","label":"ARTIFACT","kind":"range","features":[{"begin":1,"end":3,"feature_id":"range_feature_1","type":"ARTIFACT","name":"expression tag","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MKS_1","provenance_source":"PDB","original_begin":1,"original_end":3},{"begin":100,"end":101,"feature_id":"range_feature_2","type":"ARTIFACT","name":"linker","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MKS_1","provenance_source":"PDB","original_begin":100,"original_end":101},{"begin":334,"end":340,"feature_id":"range_feature_3","type":"ARTIFACT","name":"expression 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4","value":null,"source":"UNIPROT","source_id":"Q9NPP4","provenance_source":null,"original_begin":1,"original_end":96},{"begin":103,"end":332,"feature_id":"PRO_0000192576","type":"CHAIN","name":null,"description":"Green fluorescent protein","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":1,"original_end":230}]},{"key":"mutagenesis","label":"MUTAGENESIS","kind":"point","features":[{"begin":132,"end":132,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with H-145. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with H-69 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with L-46; H-69 and H-145. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":141,"end":141,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":39,"original_end":39},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; H-69 and H-145. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-64; N-68; Q-162; A-163; V-167 and L-171. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with A-72. Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. 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In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. 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In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. 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In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and V-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":277,"end":277,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPA; leads to enhanced fluorescence at 37 degrees Celsius; when associated with A-163. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":278,"end":278,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":295,"end":295,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. 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In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and F-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Results in significantly red-shifted excitation and emission maxima.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":308,"end":308,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and S-198. 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4","value":null,"source":"UNIPROT","source_id":"Q9NPP4","provenance_source":null,"original_begin":1,"original_end":96},{"begin":103,"end":332,"feature_id":"PRO_0000192576","type":"CHAIN","name":null,"description":"Green fluorescent protein","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":1,"original_end":230}]},{"key":"mutagenesis","label":"MUTAGENESIS","kind":"point","features":[{"begin":132,"end":132,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with H-145. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with H-69 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with L-46; H-69 and H-145. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":141,"end":141,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":39,"original_end":39},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; H-69 and H-145. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-64; N-68; Q-162; A-163; V-167 and L-171. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with A-72. Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. 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In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":252,"end":252,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. 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In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":39,"original_end":39},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; H-69 and H-145. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-64; N-68; Q-162; A-163; V-167 and L-171. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with A-72. Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":249,"end":249,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":250,"end":250,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":251,"end":251,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":252,"end":252,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":255,"end":255,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. 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In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":249,"end":249,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":250,"end":250,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":251,"end":251,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":252,"end":252,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":255,"end":255,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and V-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":277,"end":277,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPA; leads to enhanced fluorescence at 37 degrees Celsius; when associated with A-163. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":278,"end":278,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":295,"end":295,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":304,"end":304,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In H9; increases fluorescence at 395 nm excitation; when associated with I-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":202,"original_end":202},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to yellow-emission variants. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and A-72. 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Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. 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In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":249,"end":249,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":250,"end":250,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":251,"end":251,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":252,"end":252,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":255,"end":255,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":273,"end":273,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; A-163 and V-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":171,"original_end":171},{"begin":277,"end":277,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPA; leads to enhanced fluorescence at 37 degrees Celsius; when associated with A-163. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":175,"original_end":175},{"begin":278,"end":278,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and I-198.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":176,"original_end":176},{"begin":295,"end":295,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":193,"original_end":193},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with H-145 and A-176.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":300,"end":300,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":198,"original_end":198},{"begin":304,"end":304,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In H9; increases fluorescence at 395 nm excitation; when associated with I-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":202,"original_end":202},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to yellow-emission variants. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and A-72. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68 and A-72. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and R-79. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; A-163 and G-175.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Suppresses the 475 nm excitation peak, leaving only the shorter wavelength peak at 399 nm. In H9; increases fluorescence at 395 nm excitation; when associated with I-203. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and F-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":305,"end":305,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Results in significantly red-shifted excitation and emission maxima.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":203,"original_end":203},{"begin":308,"end":308,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; V-171 and S-198. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-206.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":206,"original_end":206},{"begin":308,"end":308,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Abolishes the tendency to dimerize and leads to monomeric fluorescent proteins.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":206,"original_end":206},{"begin":324,"end":324,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Suppresses the 399 nm excitation peak, leaving only the longer wavelength peak at 475 nm.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":222,"original_end":222},{"begin":326,"end":326,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and I-150. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198 and V-206.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":224,"original_end":224}]},{"key":"other_artifact","label":"ARTIFACT","kind":"range","features":[{"begin":1,"end":3,"feature_id":"range_feature_1","type":"ARTIFACT","name":"expression tag","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MKS_1","provenance_source":"PDB","original_begin":1,"original_end":3},{"begin":100,"end":101,"feature_id":"range_feature_2","type":"ARTIFACT","name":"linker","description":null,"value":null,"source":"PDB_ENTITY","source_id":"6MKS_1","provenance_source":"PDB","original_begin":100,"original_end":101},{"begin":334,"end":340,"feature_id":"range_feature_3","type":"ARTIFACT","name":"expression 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4","value":null,"source":"UNIPROT","source_id":"Q9NPP4","provenance_source":null,"original_begin":1,"original_end":96},{"begin":103,"end":332,"feature_id":"PRO_0000192576","type":"CHAIN","name":null,"description":"Green fluorescent protein","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":1,"original_end":230}]},{"key":"mutagenesis","label":"MUTAGENESIS","kind":"point","features":[{"begin":132,"end":132,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with H-145. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with H-69 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with L-46; H-69 and H-145. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":141,"end":141,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":39,"original_end":39},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; H-69 and H-145. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-64; N-68; Q-162; A-163; V-167 and L-171. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with A-72. Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. 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In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. 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In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. 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4","value":null,"source":"UNIPROT","source_id":"Q9NPP4","provenance_source":null,"original_begin":1,"original_end":96},{"begin":103,"end":332,"feature_id":"PRO_0000192576","type":"CHAIN","name":null,"description":"Green fluorescent protein","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":1,"original_end":230}]},{"key":"mutagenesis","label":"MUTAGENESIS","kind":"point","features":[{"begin":132,"end":132,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with H-145. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with H-69 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with L-46; H-69 and H-145. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":141,"end":141,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":39,"original_end":39},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; H-69 and H-145. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-64; N-68; Q-162; A-163; V-167 and L-171. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with A-72. Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. 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In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":252,"end":252,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. 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In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":30,"original_end":30},{"begin":141,"end":141,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; H-66; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":39,"original_end":39},{"begin":148,"end":148,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; H-69 and H-145. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-64; N-68; Q-162; A-163; V-167 and L-171. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-64; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":46,"original_end":46},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with T-65. In EBFP; gives rise to variants with blue fluorescence; when associated with T-65 and H-66. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with T-65; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighterH than ECFP; when associated with T-65; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; N-68; Q-162; A-163; V-167 and L-171. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In VisGreen; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; G-65; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":166,"end":166,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with G-65 and L-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":64,"original_end":64},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-68; A-72 and Y-203. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with A-72. Highly fluorescent; when associated with Y-203; L-68 and A-72. In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and L-69. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with L-68; A-72 and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with A-72; R-79 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; L-68; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with L-68 and A-72.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":167,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence, photostability and shifts the major exitation peak to 488 nm. In EGFP; increases fluorescence at warmer temperatures such as 37 degrees Celsius; when associated with L-64. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and H-66. In GFPmut 1; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with T-65. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; W-66; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; W-66; A-72; A-145; I-146; D-148; T-153 and A-163. In Esmerald; leads to brighter fluorescence; when associated with A-72; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; A-72; K-149 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":65},{"begin":167,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Gives rise to variants with cerulean fluorsecence.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":65,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In BFP; shifts the excitation and emission spectra to shorter wavelengths. In EBFP; gives rise to variants with blue fluorescence; when associated with L-64 and T-65. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-80; F-145; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; A-72; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Shifts the excitation and emission spectra to shorter wavelengths.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":168,"end":168,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In W; leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; I-146; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; A-72; A-145; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":66,"original_end":66},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; A-72 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and A-72. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with M-69; A-72 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; A-72; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":170,"end":170,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; Q-162; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":68,"original_end":68},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P4; leads to no detectable fluorescence. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-145. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":171,"end":171,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In RSGFP4; increases fluorescence and shifts the major exitation peak to 489-490 nm; when associated with M-64 and G-65.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":69,"original_end":69},{"begin":174,"end":174,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. In EYFP; leads to yellow fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with G-65; L-68 and Y-203. In GFPmut 2; red-shifts by about 100 nm the excitation maxima, permitting efficient excitation at 488 nm and increases fluorescence; when associated with A-65 and L-68. In Citrinine; leads to excitation and emission peaks of 516 and 529 nm, respectively; when associated with L-68; M-69 and Y-203. In YFP 10C; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; L-68; and Y-203. In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; R-79 and Y-203. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-145; I-146; D-148; T-153 and A-163. In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with F-145 and I-203. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; T-105; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224. In Esmerald; leads to brighter fluorescence; when associated with T-65; K-149; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; T-153; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":72,"original_end":72},{"begin":181,"end":181,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Topaz; shifts the major emission and exitation peak up to 20 nm; when associated with G-65; A-72 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":79,"original_end":79},{"begin":182,"end":182,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; F-145; I-150 and R-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":80,"original_end":80},{"begin":201,"end":201,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":99,"original_end":99},{"begin":205,"end":205,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with H-145.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":103,"original_end":103},{"begin":207,"end":207,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; F-145; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; V-128; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":105,"original_end":105},{"begin":230,"end":230,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; F-145; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":128,"original_end":128},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Sapphire/H9-40; exhibits a huge Stoke's shift, with an excitation peak at 399 nm and an emission peak at 511 nm; when associated with A-72 and I-203. In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; I-150 and R-224. In EBFP1.2; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-171; S-198 and V-206. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; I-150; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.3; shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5; shifts fluorescence lifetime from 3.03 to 2.72 ns; when associated with A-193. In mut1.27; shifts fluorescence lifetime from 3.03 to 2.85 ns; when associated with E-103. In mut1.28; shifts fluorescence lifetime from 3.03 to 2.76 ns; when associated with R-30. In mut2.1; shifts fluorescence lifetime from 3.03 to 2.50 ns; when associated with A-176 and I-198. In mut2.2; shifts fluorescence lifetime from 3.03 to 1.94 ns; when associated with R-30 and H-69. In mut3.3; shifts fluorescence lifetime from 3.03 to 1.88 ns; when associated with R-30; L-46 and H-69.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In mut1.9; shifts fluorescence lifetime from 3.03 to 2.74 ns.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":247,"end":247,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; I-146; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":145,"original_end":145},{"begin":248,"end":248,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; T-153 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; D-148; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":146,"original_end":146},{"begin":249,"end":249,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":147,"original_end":147},{"begin":250,"end":250,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; T-153 and A-163.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":148,"original_end":148},{"begin":251,"end":251,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; T-153 and T-167. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and T-167.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":149,"original_end":149},{"begin":252,"end":252,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In Azurite; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with H-66; R-80; F-145 and R-224. In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; V-155; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":150,"original_end":150},{"begin":255,"end":255,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and A-163. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and A-163. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and A-163. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-167. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; A-163; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":153,"original_end":153},{"begin":257,"end":257,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In EBFP2.0; shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP; when associated with R-30; N-39; H-66; A-72; T-105; V-128; F-145; I-150; V-171; S-198; V-206 and V-224.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":155,"original_end":155},{"begin":264,"end":264,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; A-163; V-167 and L-171.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":162,"original_end":162},{"begin":265,"end":265,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In GFPB; leads to enhanced fluorescence at 37 degrees Celsius. In GFPA; leads to even higher fluorescence at 37 degrees Celsius than GFPA; whenassociated with G-175. In alphaGFP/cycle 3 GFP; improves folding at 37 degrees Celsius, reduces aggregation at high concentrations, and increases the diffusibility of the protein inside cells; when associated with S-99 and T-153. In R10-3; matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm, respectively; when associated with L-46; L-64; N-68; Q-162; V-167 and L-171. In ECFP; leads to cyan fluorescence, folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness; when associated with L-64; T-65; W-66; I-146 and T-153. In Cerulean; leads to improved quantum yield, a higher extinction coefficient and is 2.5-fold brighter than ECFP; when associated with L-64; T-65; W-66; A-72; A-145; I-146; D-148 and T-153. In Venus; leads to yellow fluorescence, improved maturation and reduced environmental sensitivity; when associated with L-46; L-64; G-65; L-68; A-72; T-153; G-175 and Y-203.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":163,"original_end":163},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P11; increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald; leads to brighter fluorescence; when associated with T-65; A-72; K-149 and T-153. In VisGreen; leads to brighter fluorescence; when associated with L-64; T-65; A-72 and K-149.","value":null,"source":"UNIPROT","source_id":"P42212","provenance_source":null,"original_begin":167,"original_end":167},{"begin":269,"end":269,"feature_id":null,"type":"MUTAGENESIS_SITE","name":null,"description":"In P9; increases fluorescence at 475 nm excitation. 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